The use of anisotropic cell sheets to control orientation during the self-organization of 3D muscle tissue

Takahashi, Hironobu; Shimizu, Tatsuya; Nakayama, Masamichi; Yamato, Masayuki; Okano, Teruo

doi:10.1016/j.biomaterials.2013.06.033

Cited by 122 publications

(107 citation statements)

References 32 publications

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“…19 Additional studies used a combination of microfabrication tools (imprinting or electron-beam lithography) and chemical processes. [14][15][16]20 However, these strategies involve a shifter membrane to move the cell sheet lifted from the thermoresponsive culture substrate to the target, which may cause deformation of the aligned structure of the cell sheet. In addition, microfabrication directly onto the culture substrate is a time-consuming process with high cost.…”

Section: Discussionmentioning

confidence: 99%

“…[14][15][16] In addition, thermoresponsive polymer brush and graphene nanoribbon have been used to produce topographical and chemical patterns. [17][18][19][20][21] However, these methods require the use of shifter membrane, which complicates translocation of the harvested cell sheet. As an alternative, we have previously developed a thermoresponsive hydrogel with cell adhesion molecules to transfer cell sheets to the target in a single step and without additional transfer tools by simply decreasing the temperature from 37 to 4 o C within 10 min.…”

Section: Introductionmentioning

confidence: 99%

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Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

et al. 2016

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Although many efforts have been made to engineer cell sheets with anisotropic patterns, current techniques still exhibit several shortcomings including uncontrolled harvest time and deformation of pattern by contraction. In this study, we report a simple method to harvest a cell sheet with a striated structure of extracellular matrix (ECM) and myoblasts using a thermosensitive hydrogel micropatterned with different sizes (25 and 80 μm). The hydrogel supported the formation of a confluent monolayer of myoblasts with aligned morphology. When the temperature was reduced from 37 to 4 o C, the size of the hydrogel increased by approximately 1.2-fold within 10 min. In response to this change, a cell sheet was harvested and could be transferred to the desirable substrate through conformal contact between the hydrogel and target. We further examined the effect of pattern size on alignment of ECM/ cell assembly by fast Fourier transform (FFT) analysis of fluorescently stained stress fibers and ECM proteins within the harvested cell sheet. The cell sheet maintained alignment of confluent myoblasts after being harvested to the substrate. In addition, the topologic patterns also promoted formation of aligned myotube on the harvested cell sheet, which was important for muscle tissue regeneration. However, the pattern size appeared to have no influence on alignment of cells on the cell sheet. Finally, a bi-layered structure was fabricated in which each layer was stacked with aligned direction on the cell sheet being perpendicularly assembled. Collectively, our platform could be used for rapid harvest of cell sheets with aligned architecture of ECM/cell, which can be applied to fabrication of welldefined 3D tissue for tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

et al. 2016

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“…Engineering of cell sheets could also be a potential tool for constructing scaffold-free, three-dimensional tissues using the more responsive polymers. 26,27 …”

Section: Applications Of Nanoengineered Scaffolds In Tissue Growth Anmentioning

confidence: 99%

Emerging nanotechnology approaches in tissue engineering and regenerative medicine

Kim¹,

Ahn²,

Dvir³

et al. 2014

IJN

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“…Since the mechanical function of the myocardial tissue is the sum of the contraction forces of every cardiomyocyte, if the orientation is not aligned, efficient contraction force can not be expected. While we previously reported that we succeeded in aligning the orientation of myoblasts in myoblast sheets to some extent [36], how we align the orientation of myocardial cells efficiently is currently under investigation.…”

Section: Current Limitations and Future Perspectivesmentioning

confidence: 99%

Cell Sheet-Based Vascularized Myocardial Tissue Fabrication

2018

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Background: The development of regenerative medicine in recent years has been remarkable as tissue engineering technology and stem cell research have advanced. The ultimate goal of regenerative medicine is to fabricate human organs artificially. If fabricated organs can be transplanted medically, it will be the innovative treatment of diseases for which only donor organ transplantation is the definitive therapeutic method at present. Summary: Our group has reported successful fabrication of thick functional myocardial tissue in vivo and in vitro by using cell sheet engineering technology which requires no scaffolds. Thick myocardial tissue can be fabricated by stacking cardiomyocyte sheets on the vascular bed every 24 h, so that a vascular network can be formed within the myocardial graft. We call this procedure a multi-step transplantation procedure. After human-induced pluripotent stem cells were discovered and human cardiomyocytes became available, a thick, macroscopically pulsate human myocardial tissue was successfully constructed by using a multi-step transplantation procedure. Furthermore, our group succeeded in fabricating functional human myocardial tissue which can generate pressure. Here, we present our way of fabricating human myocardial tissue by means of cell sheet engineering technology. Key Messages: Our group succeeded in fabricating thick, functional human myocardium which can generate pulse pressure. However, there are still a few problems to be solved until clinically functional human cardiac tissue or a whole heart can be fabricated. Research on myocardial regeneration progresses at such a pace that we believe the products of this research will save many lives in the near future.

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The use of anisotropic cell sheets to control orientation during the self-organization of 3D muscle tissue

Cited by 122 publications

References 32 publications

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

Fabrication of cell sheets with anisotropically aligned myotubes using thermally expandable micropatterned hydrogels

Emerging nanotechnology approaches in tissue engineering and regenerative medicine

Cell Sheet-Based Vascularized Myocardial Tissue Fabrication

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