The Use of Agmatine Provides the New Insight in an Experimental Model of Multiple Sclerosis

Ninković, Milica; Stevanović, Ivana; Stojanović, Ivana; Ljubisavljević, Srdjan; Bašić, Jelena; Peric, Predrag

doi:10.1007/s11064-015-1655-5

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…Polyamines contribute to cell proliferation, hypertrophy and tissue development. In addition to its functions in other tissues, polyamines also have a variety of tissue-specific roles that affect neuronal excitability by regulating ion channels and receptors in brain tissue (9)(10)(11)(12)(13)(14). However, despite all this information, the functions of the endogenous polyamines in CNS are still unknown.…”

Section: Introductionmentioning

confidence: 99%

The Possible Effects of Polyamines in Multiple Sclerosis Patients on New Lesion Development and Disability

Bolayir

Çelık

Bolayır

et al. 2018

Int. J. Res. Granthaalayah

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Background: Multiple sclerosis (MS) is a demyelinating autoimmune disease characterized by the infiltration of T cells into the central nervous system. Polyamines, which contribute to cell proliferation, hypertrophy and tissue development, have some tissue-specific roles in brain tissue. So, we aimed in this study to present the possible effects of polyamines on MS. Method: Thirty- five patients with MS and 35 sex and age-matched control were included in this study. Arginine decarboxylase (ADC), ornithine decarboxylase (ODC) and agmatinase levels were measured by ELISA kits. Results: The patient group had higher ODC and agmatinase levels than controls. The correlation analyses between ODC, ADC, and agmatinase levels and disease duration were revealed that there was a negligible positive relationship between disease duration and agmatinase, with negligible negative relationship between disease duration and ODC and ADC. Additionally, there were showed respectively moderate and weak positive correlations between EDSS (Expanded Disability Status Scale) scores and newly developed lesions and ODC and agmatinase levels. Conclusions: Elevated polyamine synthesis in MS patients was presented by detecting increased ODC, ADC and agmatinase levels compared to controls. Besides this increased polyamine synthesis in MS patients was also related with disease duration, number of newly developed lesions and disability.

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Section: Introductionmentioning

confidence: 99%

The Possible Effects of Polyamines in Multiple Sclerosis Patients on New Lesion Development and Disability

Bolayir

Çelık

Bolayır

et al. 2018

Int. J. Res. Granthaalayah

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“…2 Its exceptional modulatory effects on a number of molecular targets have been demonstrated, 2 including those involved in neurotransmitter systems, 2,3 nitric oxide synthesis, 2,4 and polyamine metabolism, 2,4 highlighting the potential therapeutic applications of agmatine. 2,5,6 As a result, the importance of agmatine production is increasingly being recognised in the pharmaceutical industry. Currently, agmatine can be industrially produced by chemical synthesis from 1,4-butanediamine and 1,4-butylenedibromide.…”

Section: Introductionmentioning

confidence: 99%

Efficient agmatine production using an arginine decarboxylase with substrate‐specific activity

Sun

Song

Qiao

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND: Agmatine is a valuable pharmaceutical intermediate biosynthesised from L-arginine by arginine decarboxylase (EC 4.1.1.19). It has various potential therapeutic functions in neurotransmitter systems, nitric oxide synthesis, and polyamine metabolism. To establish a green and efficient process for agmatine production, a novel arginine decarboxylase (SpA9) from Shewanella putrefaciens was identified, overexpressed, and functionally characterised in this study. RESULTS:The SpA9 gene from S. putrefaciens was overexpressed in Escherichia coli BL21(DE3), and the recombinant SpA9 was purified 7.7-fold. The recombinant SpA9 exhibited a higher K cat /K m value (4.8 s −1 mM −1 ). Furthermore, SpA9 showed the highest activity at pH 8.5 and 37 ∘ C towards L-arginine. The highest enzyme activity of 1281 U mL −1 was achieved by optimising the nutrient, culture, and induction conditions. Under optimum transformation conditions, the maximum conversion yield of agmatine was 92.6%, with a space-time yield of 276.88 g L −1 ·day −1 at a 1 L scale. CONCLUSIONS: To our best knowledge, this bioprocess produced the highest yield and conversion rate of any biocatalyst method reported yet. It is also a greener and more cost-effective method for agmatine production compared with other available methods in the pharmaceutical industry.

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“…Due to the widespread presence of agmatine in the peripheral and central nervous system, it is conjectured to be a "magical shotgun" -a non-selective drug with multiple targets, potentially leading to more effective treatments 11,12 . AGM function in the central nervous system includes antidepressant-like effects 13,14 , protection against schizophrenia 15 , improvement of cognitive dysfunction in Alzheimer's disease 16 , and improvement of multiple sclerosis 17 .…”

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confidence: 99%

A fluorescent biosensor for the visualization of agmatine

Kröger,

Stiel,

Stüven

et al. 2024

Preprint

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Agmatine is known to regulate various neurotransmitter systems, yet the underlying molecular mechanisms remain elusive. To visualize agmatine dynamics in cellular networks and thereby unravel its physiological functions, we developed a genetically encoded fluorescent agmatine biosensor (AGMsen) based on the periplasmic putrescine binding protein PotF. We first analyzed the agmatine binding properties of the PotF receptor module based on its crystal structure and introduced mutations into the binding pocket to favor agmatine binding over other biogenic amines. Validation of AGMsen functionality across different cell types, including primary neuronal cultures, demonstrates its ability of real-time agmatine visualization. Thus, the sensor can serve as a valuable tool to advance our understanding of agmatine distribution and dynamics, and shed light on their effect on neuronal functions in vivo.

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The Use of Agmatine Provides the New Insight in an Experimental Model of Multiple Sclerosis

Cited by 7 publications

References 44 publications

The Possible Effects of Polyamines in Multiple Sclerosis Patients on New Lesion Development and Disability

The Possible Effects of Polyamines in Multiple Sclerosis Patients on New Lesion Development and Disability

Efficient agmatine production using an arginine decarboxylase with substrate‐specific activity

A fluorescent biosensor for the visualization of agmatine

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