The Use of Adjuvant and Sarcoma 180 Cells in the Production of Mouse Hyperimmune Ascitic Fluids to Arboviruses *

“…An advantage of this system is that there is no need for enzyme-conjugated antibodies for all the secondary antibodies used, and that for secondary antibodies one can use hyperimmune mouse ascitic fluids (Tikasingh et al, 1966), available in almost all arbovirus diagnostic laboratories and from the Centers for Disease Control (Atlanta, Georgia, or Ft. Collins, Colorado). The principal disadvantage of the double-sandwich ELISA is the need for an additional step and the possible increase in attendant nonspecific reactivity.…”

Section: Direct Antigen Detectionmentioning

confidence: 99%

Togaviridae and Flaviviridae: The Alphavirases and Flaviviruses

Calisher¹,

Monath²

1988

Laboratory Diagnosis of Infectious Diseases Principles and Practice

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“…On day 26 these mice were injected with Sarcoma 180 cells (approx. 0.1 ml of a 10% suspension) (Tikasingh et al, 1966). Antibody-rich ascitic fluid was collected after approximately 7-14 d accumulation.…”

Section: Methodsmentioning

confidence: 99%

Is cyanophycin involved in the integration of nitrogen and carbon metabolism in the cyanobacteria Anabaena cylindrica and Gleothece grown on light/dark cycles?

Mackerras

Youens

Weir

et al. 1990

Journal of General Microbiology

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In Anabaena cylindrica, protein synthesis continued during dark periods at 80% of the rate observed in the light. Since nitrogen fixation ceases in the dark, this implies that fixed nitrogen accumulates during the light periods to supply amino acids for protein synthesis in the dark. Measurements of cyanophycin and the distribution of nitrogen in subcellular fractions indicated that cyanophycin does not represent a significant proportion of total cell nitrogen, nor does its concentration vary across the light/dark cycle. Similar results were obtained with Gloeothece; there was no evidence that cyanophycin accumulated during the dark to support protein synthesis in the light. Cyanophycin does not, therefore, appear to serve as a dynamic temporary storage form of newly fixed nitrogen in the integration of light and dark metabolism. We also investigated an immunological approach to the measurement of cyanophycin and its turnover. Cyanophycin is immunogenic, and in the pure state could be assayed by radioimmunoassay, which had greater sensitivity than traditional assay procedures. The insolubility of cyanophycin at neutral pH, however, prevented the successful development of methods for quantifying cyanophycin turnover in cell extracts.

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The Use of Adjuvant and Sarcoma 180 Cells in the Production of Mouse Hyperimmune Ascitic Fluids to Arboviruses *

Cited by 100 publications

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Antigenic relationship of West Nile strains by titre ratios calculated from cross-neutralization test results

Antigenic relationship of West Nile strains by titre ratios calculated from cross-neutralization test results

Togaviridae and Flaviviridae: The Alphavirases and Flaviviruses

Is cyanophycin involved in the integration of nitrogen and carbon metabolism in the cyanobacteria Anabaena cylindrica and Gleothece grown on light/dark cycles?

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