The Use of Adipose Tissue-Derived Progenitors in Bone Tissue Engineering - a Review

Bhattacharya, Indranil; Ghayor, Chafik; Weber, Franz E.

doi:10.1159/000447494

Cited by 21 publications

(17 citation statements)

References 62 publications

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“…A bone tissue engineering scaffold must be osteoconductive, porous, and biodegradable to support the attachment and proliferation of bone cells and encourage bone formation [93][94][95] . Furthermore, the blood supply must be compatible with artificial scaffolds to promote new tissue growth and healing 96 . Recently, a direct-write assembly (robocasting) technique was introduced to create porous scaffolds for bone regeneration.…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Breathing life into engineered tissues using oxygen-releasing biomaterials

et al. 2019

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Engineering three-dimensional (3D) tissues in clinically relevant sizes have demonstrated to be an effective solution to bridge the gap between organ demand and the dearth of compatible organ donors. A major challenge to the clinical translation of tissue-engineered constructs is the lack of vasculature to support an adequate supply of oxygen and nutrients post-implantation. Previous efforts to improve the vascularization of engineered tissues have not been commensurate to meeting the oxygen demands of implanted constructs during the process of homogeneous integration with the host. Maintaining cell viability and metabolic activity during this period is imperative to the survival and functionality of the engineered tissues. As a corollary, there has been a shift in the scientific impetus beyond improving vascularization. Strategies to engineer biomaterials that encapsulate cells and provide the sustained release of oxygen over time are now being explored. This review summarizes different types of oxygen-releasing biomaterials, strategies for their fabrication, and approaches to meet the oxygen requirements in various tissue engineering applications, including cardiac, skin, bone, cartilage, pancreas, and muscle regeneration.

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Section: Bone Tissue Engineeringmentioning

confidence: 99%

Breathing life into engineered tissues using oxygen-releasing biomaterials

et al. 2019

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“…Diseases of the locomotive system, such as fractures, osteoporosis, rheumatoid arthritis, and others, are the second most frequent diseases after the cardiovascular diseases (Schliemann et al, 2015). Many growth factors and hormones are known for their supportive effect on bone growth (e.g., bone morphogenetic protein 2, BMP-2); nevertheless, their isolation and production can be complicated and expensive (Bhattacharya et al, 2016). Moreover, there are indications that the application of BMP-2 may lead to negative side effects (Shields et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…The human ASC were chosen as being representative of mesenchymal stem cells. Recently, it was proven that ASC have comparable morphology, phenotype, and potential differentiation ability to bone marrow mesenchymal stem cells (Levi and Longaker, 2011;Bhattacharya et al, 2016). Additionally, due to their subcutaneous localization, these cells are easily accessible by liposuction in relatively high amounts.…”

Section: Introductionmentioning

confidence: 99%

Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

Douglas

Vandrovcová

Kročilová

et al. 2018

Journal of Dairy Science

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Recently, milk-derived proteins have attracted attention for applications in the biomedical field such as tissue regeneration. Whey protein isolate (WPI), especially its main component β-lactoglobulin, can modulate immunity and acts as an antioxidant, antitumor, antiviral, and antibacterial agent. There are very few reports of the application of WPI in tissue engineering, especially in bone tissue engineering. In this study, we tested the influence of different concentrations of WPI on behavior of human osteoblast-like Saos-2 cells, human adipose tissue-derived stem cells (ASC), and human neonatal dermal fibroblasts (FIB). The positive effect on growth was apparent for Saos-2 cells and FIB but not for ASC. However, the expression of markers characteristic for early osteogenic cell differentiation [type-I collagen (COL1) and alkaline phosphatase (ALP)] as well as ALP activity, increased dose-dependently in ASC. Importantly, Saos-2 cells were able to deposit calcium in the presence of WPI, even in a proliferation medium without other supplements that support osteogenic cell differentiation. The results indicate that, depending on the cell type, WPI can act as an enhancer of cell proliferation and osteogenic differentiation. Therefore, enrichment of biomaterials for bone regeneration with WPI seems a promising approach, especially due to the low cost of WPI.

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“…Biomaterials that chemically interact with the host tissue are bioactive. Materials that are resorbed over time are biodegradable [54,55]. AL sponges can be considered suitable as scaffolds because they are biodegradable [56].…”

Section: Discussionmentioning

confidence: 99%

Hard Tissue-Forming Ability and Ultra-Micro Structure of Newly Developed Sponges as Scaffolds Made with Sodium Alginate Gel and Chondroitin Sulfate

Miyamoto¹,

Yoshikawa²,

Maeda³

2018

JBiSE

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To regenerate teeth and bones, a scaffold is essential. Hydroxyapatite has been used in many studies, but scaffolds made of hydrogel or sponge are also effective. The hardness of hydroxyapatite is a disadvantageous property for shaping. A sponge is suitable as a scaffold because the shape of the scaffold can be matched to the defect. Sodium alginate (AL) has excellent biocompatibility and a sponge can be made from this gel by lyophilization. The purpose of this study was to promote hard tissue formation with a sponge made of AL gel or AL gel and chondroitin sulfate (Chs). Sponges were made from AL gel, which were then used as a scaffold to investigate their effectiveness for the formation of hard tissue or bone. Hard tissue formation in the pores of these AL sponges was estimated in vitro and in vivo. In the sponge made from AL gel, the concentration of AL and the addition of Chs affected bone formation. Concentration of AL would affect the shape and size of the pores. ALP activity in the sponges was also enhanced by Chs. The amount of osteocalcin (OC) produced in the sponge by rat bone marrow cells increased depending on the AL and Chs concentrations in the gel. The level of OC amount in the sponges made from AL gel containing Chs was notable in vivo. Bone formation in the sponge in vivo was affected by the addition of Chs in AL gel. The quantity of OC and the bone formation in AL sponges in subcutaneous tissue in vivo suggested that AL sponges can be useful as a scaffold. 2.2. Preparation of AL Sponge Scaffolds and Microstructural Observation by SEM Each gel was poured into a ring made of stainless steel with a 6-mm inner diameter, 8-mm outer

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The Use of Adipose Tissue-Derived Progenitors in Bone Tissue Engineering - a Review

Cited by 21 publications

References 62 publications

Breathing life into engineered tissues using oxygen-releasing biomaterials

Breathing life into engineered tissues using oxygen-releasing biomaterials

Application of whey protein isolate in bone regeneration: Effects on growth and osteogenic differentiation of bone-forming cells

Hard Tissue-Forming Ability and Ultra-Micro Structure of Newly Developed Sponges as Scaffolds Made with Sodium Alginate Gel and Chondroitin Sulfate

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