The use of acid phosphatase test papers for DNA profiling

Reshef, Ayeleth; Barash, Mark; Gallili, Nira; Michael, A; Brauner, Paul

doi:10.1016/s1355-0306(05)71635-x

Cited by 6 publications

(1 citation statement)

References 5 publications

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“…In those cases where it may be necessary to carry out a presumptive test (Phadebas, for instance), we first tape an area, to avoid the loss of cellular material and only subsequently carry out the presumptive test. This loss of cellular material has been observed in the acid phosphatase presumptive test in which sperm cells have been shown to diffuse from casework items to filter papers (13,14).…”

Section: Discussionmentioning

confidence: 79%

The Use of Adhesive Tape for Recovery of DNA from Crime Scene Items

Barash

Reshef

Brauner

2010

Journal of Forensic Sciences

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The selection of the appropriate method of collection of biological material from crime scene items can be crucial to obtaining a DNA profile. The three techniques commonly used for sampling items are: cutting, swabbing, and taping. The tape sampling technique offers an advantage, in that it enables the collection of a potentially highly informative source of DNA, shed epithelial cells, from selected areas on crime scene items (the inside fingers of a glove, for instance). Furthermore, surface collection of biological material by taping reduces co-sampling of known PCR inhibitors such as clothing dyes. The correct choice of tape for crime scene item sampling is important. Not all tapes are suitable for biological trace evidence collection as well as DNA extraction. We report on one tape that met both these criteria. Three different cases are presented which demonstrate the usefulness of adhesive tape sampling of crime items. Finally, the advantages of the tape collection technique are discussed and guidelines for preferred areas of tape sampling on various casework items are presented.

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Section: Discussionmentioning

confidence: 79%

The Use of Adhesive Tape for Recovery of DNA from Crime Scene Items

Barash

Reshef

Brauner

2010

Journal of Forensic Sciences

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DNA: Sources of

Harbison

2009

Wiley Encyclopedia of Forensic Science

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The sources of DNA evidence are many and varied. It is now well recognized that DNA profiles can be obtained from samples that are too small to be seen and sometimes difficult or impossible to attribute to a particular cell type. Thus, the ability to obtain a DNA profile is limited more by the ability to locate and identify potential evidence than it is by the evidence itself. DNA can be obtained from traces of sweat left on touched items, from saliva deposited on the backs of stamps, and from pollen transferred from one location to another, as well as from blood splashed and smeared at a crime scene. In this article, by reference to case reports and articles published over the last 20 years and by drawing on this author's personal experiences and those of others as practicing forensic scientists, attempts have been made to convey the huge variety of sources of DNA evidence that can be encountered, recognizing the infinite variety of samples, substrates, and case circumstances that exist.

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