The Use of a Rotating Drum for the Study of Aerosols Over Extended Periods of Time1

Goldberg, L. J.; Watkins, H. M. S.; Boerke, E. E.; Chatigny, M. A.

doi:10.1093/oxfordjournals.aje.a119954

Cited by 112 publications

(93 citation statements)

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“…The drum and contained cloud were rotated at 3 r.p.m. to reduce physical loss by sedimentation (Goldberg et al 1958).…”

Section: Methodsmentioning

confidence: 99%

“…The recent development of more accurate viral assay techniques; the introduction of the rotating drum for long-term cloud holding (Goldberg, Watkins, Boerke & Chatigny, 1958); and the use of tracer methods for measuring physical decay in aerosols (Harper, Hood & Morton, 1958) make it possible to carry out studies which should be readily repeatable in other laboratories. This paper reports the influence of R.Hl.…”

Section: Introductionmentioning

confidence: 99%

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Airborne micro-organisms: survival tests with four viruses

1961

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Airborne virus particles of vaccinia, influenza, Venezuelan equine encephalomyelitis, and poliomyelitis have been tested for viable survival in the dark at controlled temperatures and relative humidity (R.H.) for up to 23 hr. after spraying.Viable survival at each R.H. level was better at lower temperature than at higher temperature. Poliomyelitis virus showed best survival at high R.H.; the other three viruses survived best at low R.H.

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“…The drum and contained cloud were rotated at 3 r.p.m. to reduce physical loss by sedimentation (Goldberg et al 1958).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Airborne micro-organisms: survival tests with four viruses

1961

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“…A suspension of PRRSV was aerosolized into a dynamic aerosol toroid (DAT) [16] rotating at 5 revolutions per minute and maintained at a pre-determined temperature and relative humidity. The cloud of PRRSV contained within the DAT was sampled repeatedly over time, the concentration of infectious PRRSV (TCID 50 ) in the samples was determined, and the T 1/2 for the specific combination of relative humidity and temperature was estimated based on the inactivation of infectious virus observed over time.…”

Section: Experimental Designmentioning

confidence: 99%

“…1) measured 24 inches (60.9 cm) in diameter by 18 inches (45.7 cm) deep, thus providing a total volume of 133 L. A variable speed motor (BHLW15L-120T-D2, Brother Gearmotors, Bridgewater, NJ, USA) rotated the DAT at 5 revolutions per minute (RPMs). The rotation of the DAT maintained the aerosolized particles in suspension and reduced the rate of physical loss through sedimentation [16,37,39]. Three ports, equally spaced on the circumference of the DAT and equipped with externally mounted hepa-filters (Fisher Scientific, 18-999-2574, Hampton, NH, USA), allowed for pressure equilibration during nebulization (introduction of air) and impingement (extraction of air).…”

Section: Dynamic Aerosol Toroid (Dat)mentioning

confidence: 99%

Effect of temperature and relative humidity on the stability of infectious porcine reproductive and respiratory syndrome virus in aerosols

et al. 2007

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-The objective of this experiment was to describe the stability of airborne infectious porcine reproductive and respiratory syndrome virus (PRRSV) as a function of temperature and relative humidity. A cloud of infectious PRRSV was aerosolized using 24-jet Collison nebulizer into a dynamic aerosol toroid (DAT) maintained at a specific temperature and relative humidity. The PRRSV cloud within the DAT was sampled repeatedly over time using SKC BioSampler impingers and the total viral RNA (RT-PCR) and concentration of infectious PRRSV (TCID 50 ) in the air samples was determined. As measured by quantitative RT-PCR, PRRSV RNA was stable under the conditions evaluated in this study. Thus, a comparison of viral RNA and Rhodamine B dye, a physical tracer, found no significant difference in the slopes of the lines. Titers of infectious virus were plotted by time and the half-life (T 1/2) of infectious PRRSV was calculated using linear regression analysis. An analysis of the results showed that aerosolized PRRSV was more stable at lower temperatures and/or lower relative humidity, but temperature had a greater effect on the T 1/2 of PRRSV than relative humidity. Based on these results, an equation was derived to predict the T 1/2 of infectious airborne PRRSV for any combination of environmental temperature and relative humidity.aerosol / virus stability / PRRSV / relative humidity / temperature

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“…Aerosols were generated with a 3-jet Collison nebulizer into a Henderson apparatus, modified to operate over a range of relative humidity values (Druett, 1969), and stored in a 55 1 rotating drum (Goldberg et al 1958). Samples were recovered from aerosols for 1 min, using raised impingers with a flow rate of 10-5 1/min (May & Harper, 1957).…”

Section: Organismsmentioning

confidence: 99%

Survival of virulentLegionella pneumophilain aerosols

Hambleton

Broster²,

Dennis

et al. 1983

J. Hyg.

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SUMMARYAqueous suspensions of virulent Legionella pneumophila grown on solid medium retained virulence and aerosol survival characteristics for several months. Significant numbers of viable organisms were recovered from aerosols held at various relative humidities (r.h.) for up to 2 h. The organisms survived best at 65% r.h. and were least stable at 55 % r.h.Exponential phase broth-grown organisms survived poorly in aerosols in comparison with stationary phase broth cultures or organisms grown on solid medium, suggesting that the metabolic status of Legionella pneumophila organisms may be an important factor affecting their ability to survive in aerosols and cause respiratory disease.

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The Use of a Rotating Drum for the Study of Aerosols Over Extended Periods of Time1

Cited by 112 publications

References 0 publications

Airborne micro-organisms: survival tests with four viruses

Airborne micro-organisms: survival tests with four viruses

Effect of temperature and relative humidity on the stability of infectious porcine reproductive and respiratory syndrome virus in aerosols

Survival of virulentLegionella pneumophilain aerosols

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