The use of a pharmabiotic based on the Lactobacillus fermentum U-21 strain to modulate the neurodegenerative process in an experimental model of Parkinson disease

Даниленко, В. Н.; Valery, N Danilenko; Stavrovskaya, A. V.; Alla, V Stavrovskaya; Воронков, Д. Н.; Dmitriy, N Voronkov; Gushchina, A. S.; Anastasiya, S Gushchina; Марсова, М. В.; Maria, Veit; Yamshchikova, N. G.; Nina, G.; Olshansky, A. S.; Аrtyem, S Ol’shansky; Ivanov, M. V.; Иллариошкин, С.Н.; Sergey, N Illarioshkin

doi:10.25692/acen.2020.1.7

Cited by 7 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The L. fermentum U-21 strain, isolated in the Laboratory of Microbial Genetics, Vavilov Institute of General Genetics RAS, showed high antioxidant activity in studies on in vitro models using the oxidative stress inducer paraquat [19]. In vivo experiments on a model of paraquat-induced Parkinsonism in mice showed that administration of L. fermentum U-21 improved movement coordination and prevented degradation of dopaminergic brain neurons [20], increased survival, preservation of tyrosine hydroxylase-positive nerve fibres, and the number of goblet cells in enteric nerve plexuses in rats [21]. High biological activity was also observed in the neurotoxic model of MPTP-induced Parkinsonism in mice [22].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21

Al Ebrahim,

Alekseeva,

Bazhenov

et al. 2024

Preprint

View full text Add to dashboard Cite

Strain L. fermentum U-21 secretes chaperones into the medium and it is considered as the candidate for the development of drugs, so-called disaggregases, for Parkinson’s disease therapy. This study is devoted to characterization of secreted protein encoded by C0965_000195 locus. Analysis of sequence and predicted structure of the protein encoded by C0965_000195 allows us to attribute it to the ClpL, homologs of which are known to be chaperones. The chaperone activity of ClpL L. fermentum U-21 was evaluated in vivo by refolding of differing in thermostability luciferases from Aliivibrio fischeri and Photorhabdus luminescens in Escherichia coli cells. It was observed that clpL from L. fermentum U-21 can compensate for the deficiency in the clpB gene and enhance the ability to refold thermodenatured proteins in clpB- cells. In vitro experiments have demonstrated the ability of a spent culture medium containing proteins secreted by L. fermentum U-21 cells, including ClpL, to prevent thermodenaturation of luciferases partially.We suggest that the ClpL protein from L. fermentum U-21 strain, which exhibits disaggregase properties against aggregating proteins, may be one of the clue components that play a role in the pharmabiotic properties of this strain.

show abstract

Section: Introductionmentioning

confidence: 99%

“…21 (collection number VKPM V-12075, NCBI Genome assembly ASM286982v2);• E. coli SG20250 (ΔlacU169 araD flbB relA clpB + ), and its insertion derivative SG22100 clpB::kan -…”

mentioning

confidence: 99%

ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21

Al Ebrahim,

Alekseeva,

Bazhenov

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Thus, dysbiosis of the gut microbiome often causes brain diseases. Recent experimental results described that gut microbiome dysbiosis was observed in patients with autism, Alzheimer’s disease, and Parkinson’s disease ( 7 – 10 ). At the same time, studies on mechanisms to understand the gut-brain axis were conducted.…”

Section: Introductionmentioning

confidence: 99%

Positive Effect of Lactobacillus acidophilus EG004 on Cognitive Ability of Healthy Mice by Fecal Microbiome Analysis Using Full-Length 16S-23S rRNA Metagenome Sequencing

Jeon

Kim

et al. 2022

Microbiol Spectr

View full text Add to dashboard Cite

show abstract

“…Lactobacilli are the important component of the human microbiota and, due to the active synthesis of biologically active compounds and bidirectional communication with the host organism, they can affect the state and antioxidant (AO) status of the macroorganism. The strain of Limosilactobacillus fermentum U-21 showed high AO activity on in vitro and in vivo models [1,2]. In this work, we used a combination of genomic, transcriptomic, and proteomic technologies to identify genes and proteins that potentially determine the unique AO properties of the strain.…”

mentioning

confidence: 99%

Omix technologies and bioinformatics: an example of use in creating a pharmabiotic based on the Limosilactobacillus fermentum U21 strain

2022

The Thirteenth International Multiconference

View full text Add to dashboard Cite

Motivation and Aim: Today's world is undergoing revolutionary changes in the development and use of pharmacological preparations based on bacteria and their biologically active ingredients. Such preparations are increasingly referred to as pharmabiotics, as opposed to probiotics, used primarily as dietary supplements and consumed by healthy people. Pharmabiotics are live biotherapeutic drugs and/or their metabolites and components with established pharmacological ingredients, mechanism of action and intended for treating specific diseases. In order to develop pharmabiotics, aside from traditional microbiological and biotechnological approaches, a complex of omics technologies can substantially facilitate the process. Lactobacilli are the important component of the human microbiota and, due to the active synthesis of biologically active compounds and bidirectional communication with the host organism, they can affect the state and antioxidant (AO) status of the macroorganism. The strain of Limosilactobacillus fermentum U-21 showed high AO activity on in vitro and in vivo models [1,2]. In this work, we used a combination of genomic, transcriptomic, and proteomic technologies to identify genes and proteins that potentially determine the unique AO properties of the strain. Methods and Algorithms: The DNA of the L. fermentum U21 strain and the comparison strains L. fermentum 103 and L. fermentum 279 was sequenced and deposited in the NCBI GenBank (WGS PNBB01, PGGI01, PGGE01). The search for genes exhibiting AO properties in the genome of L. fermentum U-21 was carried out using the reference catalog of genes of antioxidant proteins found in various species and strains of lactobacilli [3; https://github.com/Alexey-Kovtun/Catalog] and the developed algorithm for their search [4]. RNA was isolated on a King Fisher automated station with the MagMAX™ mirVana™ Total RNA Isolation Kit (Thermo Fisher Scientific). Ready libraries were sequenced on an Illumina HiSeq 2500. Kallisto v0.46.0 software was used to map reads and evaluate transcript abundance. Differential expression analysis was performed using the edgeR v3.26.8 package integrated into the Degust v4.1.1 web tool. The proteins of the cell-free culture supernatants of L. fermentum strains were separated in SDS-PAGE. For mass spectrometric analysis, the colored protein bands were excised and the peptides were separated using the Ultimate 3000 Nano LC system connected to a Q Exactive HF mass spectrometer through a nanoelectrospray source (Thermo Fischer Scientific) based on the mass spectrometry group of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry. RAS. LC-MS/MS data analysis was performed using PEAKS Studio 8.0 build 2016-0908 software. The primary structures of the generated peptides were analyzed based on the UNIPROT KB protein sequence database (07.2016).

show abstract

The use of a pharmabiotic based on the Lactobacillus fermentum U-21 strain to modulate the neurodegenerative process in an experimental model of Parkinson disease

Cited by 7 publications

References 0 publications

ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21

ClpL Chaperone as a Possible Component of the Disaggregase Activity of Limosilactobacillus fermentum U-21

Positive Effect of Lactobacillus acidophilus EG004 on Cognitive Ability of Healthy Mice by Fecal Microbiome Analysis Using Full-Length 16S-23S rRNA Metagenome Sequencing

Omix technologies and bioinformatics: an example of use in creating a pharmabiotic based on the Limosilactobacillus fermentum U21 strain

Contact Info

Product

Resources

About