The use of a non-cultured autologous cell suspension and Integra® dermal regeneration template to repair full-thickness skin wounds in a porcine model: A one-step process

Wood, Fiona; Stoner, Marie L.; Fowler, Barry; Fear, Mark W.

doi:10.1016/j.burns.2006.10.388

Cited by 99 publications

(64 citation statements)

References 19 publications

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“…There have been attempts to combine commercially available dermal substitutes with either cultured or non-cultured autologous cells in pre-clinical studies Boyce et al 1999;Jones et al 2003;Wood et al 2007) with promising results, but no follow-up clinical trial results are available yet. There is only one three-dimensional reconstructed skin substitute which has achieved clinical use and has been found to be very promisingthe so-called Cincinnati Shriners Skin Substitute or PermaDerm-which was designed by Boyce and colleagues (Supp & Boyce 2005;Boyce et al 2006).…”

Section: Tissuetech Autograft Systemmentioning

confidence: 99%

“…Other clinical trials also outline the importance of dermal pregraftment for the successful take of cultured autologous keratinocytes (Travia et al 2003). In vivo studies have also shown the importance of a dermal bed for successful full-thickness wound epithelialization by sprayed keratinocytes (Wood et al 2007). …”

Section: Dermal Substitutesmentioning

confidence: 99%

“…It cannot be used on infected wounds, it requires a relatively long time of 10-14 days for vascularization and also requires a second operative procedure to achieve permanent wound closure with an SSG. In attempts to achieve a single-stage surgical procedure, the product has been seeded with disaggregated cultured ( Jones et al 2003) or non-cultured (Wood et al 2007) autologous keratinocytes, using in vivo experimental models. Results were promising, but further clinical follow-up is required.…”

Section: Ez Dermmentioning

confidence: 99%

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A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

616

469

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Situations where normal autografts cannot be used to replace damaged skin often lead to a greater risk of mortality, prolonged hospital stay and increased expenditure for the National Health Service. There is a substantial need for tissue-engineered skin bioconstructs and research is active in this field. Significant progress has been made over the years in the development and clinical use of bioengineered components of the various skin layers. Off-the-shelf availability of such constructs, or production of sufficient quantities of biological materials to aid rapid wound closure, are often the only means to help patients with major skin loss. The aim of this review is to describe those materials already commercially available for clinical use as well as to give a short insight to those under development. It seeks to provide skin scientists/tissue engineers with the information required to not only develop in vitro models of skin, but to move closer to achieving the ultimate goal of an off-the-shelf, complete full-thickness skin replacement.

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Section: Tissuetech Autograft Systemmentioning

confidence: 99%

Section: Dermal Substitutesmentioning

confidence: 99%

Section: Ez Dermmentioning

confidence: 99%

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A review of tissue-engineered skin bioconstructs available for skin reconstruction

Shevchenko

James

2009

J. R. Soc. Interface.

616

469

View full text Add to dashboard Cite

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“…For most engineering materials, the ratio of Young's modulus (E) to failure stress (r f ) is at least 10:1; for metals and many plastics and polymers, the ratio is approaches or exceeds 100:1 [6]. In the case of these materials, the approximation presented will serve as a good estimate of the material's response to an induced deflection.…”

Section: Assumptionsmentioning

confidence: 95%

“…Successful production of viable, correctly functioning organs requires not only the presence of the appropriate cell types but also the ability to correctly arrange cells in relation to one another in 3D space [2][3][4][5]. Existing approaches remain limited in two ways: parallel, high-throughput methods lack the capability to achieve complex 3D microstructures [6][7][8][9]; and serial, highaccuracy, fine-resolution methods (such as 3D printing) remain expensive and rate-limited [10,11]. Origami-inspired assembly of tissue scaffolds by folding of cell-seeded sheets is one approach with the potential to achieve high-throughput and high spatial complexity at low-cost.…”

Section: Introductionmentioning

confidence: 99%

Soft Origami: Classification, Constraint, and Actuation of Highly Compliant Origami Structures

Wheeler

Culpepper

2016

Journal of Mechanisms and Robotics

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Herein, we discuss the folding of highly compliant origami structures-"Soft Origami." There are benefits to be had in folding compliant sheets (which cannot self-guide their motion) rather than conventional rigid origami. Example applications include scaffolds for artificial tissue generation and foldable substrates for flexible electronic assemblies. Highly compliant origami has not been contemplated by existing theory, which treats origami structures largely as rigid or semirigid mechanisms with compliant hinges-"mechanism-reliant origami." We present a quantitative metric-the origami compliance metric (OCM)-that aids in identifying proper modeling of a homogeneous origami structure based upon the compliance regime it falls into (soft, hybrid, or mechanism-reliant). We discuss the unique properties, applications, and design drivers for practical implementation of Soft Origami. We detail a theory of proper constraint by which an ideal soft structure's number of degrees-of-freedom may be approximated as 3n, where n is the number of vertices of the fold pattern. Buckling and sagging behaviors in very compliant structures can be counteracted with the application of tension; we present a method for calculating the tension force required to reduce sagging error below a user-prescribed value. Finally, we introduce a concept for a scalable process in which a few actuators and stretching membranes may be used to simultaneously fold many origami substructures that share common degrees-of-freedom.

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Reconstruction of Full-Thickness Scalp Defects Using a Dermal Regeneration Template

Richardson

Lange

Jordan

2016

JAMA Facial Plast Surg

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IMPORTANCE Large full-thickness scalp defects pose a reconstructive problem and commonly require microvascular free flap reconstruction. OBJECTIVE To describe a novel and effective reconstructive technique for full-thickness scalp defects that can be performed quickly without general anesthesia or free flap reconstruction. DESIGN, SETTING, AND PARTICIPANTS A retrospective review of 10 patients at a single medical center who underwent surgical resection of a cutaneous lesion. Reconstruction of the large scalp defects included application of Integra bilayer wound matrix followed by delayed split-thickness skin grafting from January 1, 2008, to December 31, 2014. Patients ranged in age from 50 to 87 (mean, 71.5) years; 8 (80%) were men. Mean duration of follow-up was 481.1 days (range, 41-1199 days). MAIN OUTCOMES AND MEASURES Skin graft viability and adherence to underlying tissue (take) and postoperative complications. RESULTS The 10 patients in this study had excellent skin graft and wound closure outcomes. Nine patients showed a 100% initial take of the skin graft to the defect site. Only 1 patient showed a 95% to 100% initial take. Adequate coverage of the wound bed was achieved with acceptable cosmetic results. Two patients underwent postoperative intensity-modulated radiotherapy. One of these patients experienced radiotherapy-induced wound breakdown 3½ months after completion of therapy, which resolved completely after more than 6 months. CONCLUSIONS AND RELEVANCE This novel technique for reconstruction of large full-thickness scalp defects has low morbidity and can be performed on an outpatient basis with minimal wound care. The technique provides the surgeon with an alternative to other reconstructive options, including microvascular free tissue transfer, for repair of large full-thickness scalp defects. The procedure has excellent results and can be performed under sedation and local anesthesia, which avoids the risks associated with general anesthesia. LEVEL OF EVIDENCE 4.

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The use of a non-cultured autologous cell suspension and Integra® dermal regeneration template to repair full-thickness skin wounds in a porcine model: A one-step process

Cited by 99 publications

References 19 publications

A review of tissue-engineered skin bioconstructs available for skin reconstruction

A review of tissue-engineered skin bioconstructs available for skin reconstruction

Soft Origami: Classification, Constraint, and Actuation of Highly Compliant Origami Structures

Reconstruction of Full-Thickness Scalp Defects Using a Dermal Regeneration Template

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