The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants

Wawrzyk, Anna; Łobacz, Michał; Adamczuk, Agnieszka; Sofińska-Chmiel, Weronika; Wilczyński, Sławomir; Rahnama, Mansur

doi:10.3390/microorganisms9112359

Cited by 7 publications

(7 citation statements)

References 40 publications

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“…One of the methods that can reduce the number of microorganisms on the composites’ surface is the use of a diode laser, the irradiation of which can supplement the implantological treatment procedure. The effectiveness of the diode laser against microorganisms inhabiting porcelain, zircon, and titanium was confirmed by Wawrzyk et al [ 20 , 21 , 22 ]. The method of decontamination with the diode laser used by Wawrzyk et.al.…”

Section: Introductionmentioning

confidence: 83%

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies

Wawrzyk

Rahnama-Hezavah

Sofińska-Chmiel

et al. 2022

IJERPH

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Patients undergoing implant treatment are at risk of peri-implant bone loss, which is most often caused by the adverse effects of microorganisms, but there are few proven procedures for their reduction. The aim of the research was to identify the microorganisms inhabiting the composites used to close the screw access hole (SAH), compare them numerically with those present on the surface of crowns and teeth, and optimize the doses of the diode laser, which will reduce microorganisms and will not deteriorate the roughness of polished composites. Patients were swabbed from the surface of SAH composites, from porcelain and zirconium restorations, and from teeth, and then the number of microorganisms was determined by using a culture technique. Microorganisms were identified by MALDI–TOF MS and NGS sequencing. The effectiveness of diode laser irradiation was achieved by using four variants of exposure. After polishing and laser irradiation, the surface roughness of the composites was studied by using optical profilometry. On the surface of SAH, 106 to 108 microorganisms were identified at 0.4 cm2, including many pathogenic species. Among the materials used for the reconstruction of dental implants, the greatest microbiological contamination was found on the composites used to close the SAH. The diode laser with a wavelength of 810 nm with an average power of 3.84 W, during 60 s and 2 × 30 s, has a biocidal effect and does not significantly change the surface roughness of composites. The best reduction of microorganisms was achieved on a composite polished with a polishing rubber and then with a Sof-Lex™ Pre-Polishing Spiral beige (3M ESPE, Ave. St. Paul., Minnesota, USA). Studies have shown that using the optimal laser dose can help treat periimplantitis. These studies provide important information on the possibility of the effective elimination of microorganisms by using a diode laser in the treatment of peri-implant bone loss.

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Section: Introductionmentioning

confidence: 83%

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies

Wawrzyk

Rahnama-Hezavah

Sofińska-Chmiel

et al. 2022

IJERPH

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“…A-BSM successfully achieves disinfection with a diode laser, where in the medical field, a reduction of bacteria and fungi from 60% to 100% was achieved on various materials [34,35].…”

Section: Discussionmentioning

confidence: 99%

Analysis of the Surface of Historic Fabric from the Auschwitz-Birkenau State Museum after Treatment with Ethanol Mist Used to Eliminate Microorganisms Harmful to Human Health

Wawrzyk,

Poskrobko,

Guzińska

et al. 2024

Materials

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Aim: the aim of the work was to present the changes occurring on the model and historical cotton surface of cotton resulting from disinfection with 90% ethanol mist. Materials and methods: Samples of historical materials consisted of fabric elements from suitcases stored in A-BSM. A mist of 90% ethanol was applied for 15 s at a distance of 16 cm from the surface. The spectra of cotton samples before and after ethanol application were recorded using Fourier transform infrared spectroscopy (FTIR-ATR). Analyses of the surface layers were performed using X-ray photoelectron spectroscopy (XPS). Results: the decontamination performed did not show any significant differences in the chemical composition and surface structure of cotton before and after the use of 90% ethanol mist. Conclusions: Ethanol mist, which eliminates microorganisms from the historical surface, does not cause significant changes to the surface of historical objects.

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“…The A-BSM successfully performs disinfection with a diode laser, for which in the medical area, a reduction of bacteria and fungi from 60% to 100% was achieved on various materials [ 18 , 19 , 20 , 21 , 22 ]; on historical collagen material in the A-BSM, a reduction of 78–92% was achieved, and on cellulose, 90–100% was achieved [ 8 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Optimization of the Process of Eliminating Microorganisms Harmful to Human Health and Threatening Objects Isolated from Historical Materials from the Auschwitz-Birkenau State Museum in Poland (A-BSM) Collection with the Use of Ethanol in the Form of Mist

et al. 2023

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The aim of the study was to assess the biocidal effectiveness and the effect of 80% and 90% ethanol applied in the form of mist on the surface of textile materials from historical A-BSM objects. The microorganisms used for the tests, namely, Cladosporium cladosporioides, Aspergillus niger and Penicillium chrysogenum, were isolated from the surface of textile objects in the A-BSM. Bacillus subtilis, Staphylococcus aureus, Aspergillus flavus and Aspergillus niger were also used from the American Type Culture Collection (ATCC). Fabric samples were inoculated with microorganisms at a concentration of 105–106 CFU/ml. Ethanol in the form of mist was applied in concentrations of 80% and 90%. Airbrushes VL 0819 and VE 0707 were used for this purpose, where the pressure was 0.2 MPa and the PA HEAD VLH-5 nozzle with a tip of 1.05 mm in diameter was used. In order to achieve more effective disinfection after applying the ethanol mist, samples were stored in PE foil in the conditions of 21 °C ± 1 °C for 22 ± 1 h. After applying the ethanol mist, changes in the properties of the materials were assessed using scanning electron microscopy (SEM). The reduction in the number of microorganisms on modern cotton fabric after the use of ethanol in the form of mist at concentrations of 80% and 90% ranged from 93.27% to 99.91% for fungi and from 94.96% to 100% for bacteria, except for 74.24% for B. subtillis. On the historical fabric, after the time of application of 90% ethanol was shortened to 4 s, the microorganisms were reduced by over 99.93% and S. aureus was completely eliminated. After applying the tested disinfection technique, no changes in fiber morphology were observed on the surface of the model and historical cotton.

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The Use of a Diode Laser for Removal of Microorganisms from the Surfaces of Zirconia and Porcelain Applied to Superstructure Dental Implants

Cited by 7 publications

References 40 publications

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies

The Use of the Diode Laser against the Microbiome on Composites Closing the Screw Access Hall (Sah) in the Reconstruction of Dental Implants: Ex Vivo Studies

Analysis of the Surface of Historic Fabric from the Auschwitz-Birkenau State Museum after Treatment with Ethanol Mist Used to Eliminate Microorganisms Harmful to Human Health

Optimization of the Process of Eliminating Microorganisms Harmful to Human Health and Threatening Objects Isolated from Historical Materials from the Auschwitz-Birkenau State Museum in Poland (A-BSM) Collection with the Use of Ethanol in the Form of Mist

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