The Use of a Dichromatic Stain Method (Spermac®) for Determining Changes in the Acrosomal Integrity of Boar Semen during Cryopreservation

Paulenz, H; Grevle, IS; Berg, K. Andersen; Thomassen, Ragnar

doi:10.1111/j.1439-0531.1995.tb00615.x

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…Simultaneously with the motility assessment, semen smears from each sample were prepared for determination of acrosomal status using the dichromatic Spermac ® stain [11,14]. After staining, each sample was examined under oil immersion and 1000 × magnification using a bright field microscope.…”

Section: Methodsmentioning

confidence: 99%

Influence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Boar Semen Stored for Five Days

Kommisrud¹,

Paulenz

Sehested³

et al. 2002

Acta Vet Scand

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Kommisrud E, Paulenz H, Sehested E, Grevle IS: Influence of boar and semen parameters on motility and acrosome integrity in liquid boar semen stored for five days. Acta vet. scand. 2002, 43, 49-55. -Ninety ejaculates from a total of 76 AI boars were extended in Beltsville Thawing Solution (BTS). Boar identity, breed, weight of the ejaculate and sperm concentration were registered. Motility and acrosome integrity were assessed after storage at 16-18°C for 6, 30, 54, 78, and 102 h. Storage time had a significant influence on both motility (p<0.01) and acrosome integrity (p<0.001). The Least Square Means for percentage of motility showed a small decline from 79.8% after 6 h of storage to 78.4% at 102 h. Motility at 78 and 102 h was significantly different from motility at 6 h (p<0.05). The percentage of sperm cells with normal acrosomes declined throughout the experiment. The Least Square Means for 6, 30, 54, 78, and 102 h of storage were 93.9%, 90.6%, 88.0%, 84.8%, and 78.2%, respectively. The decrease in acrosome integrity from one storage time to the next was highly significant throughout the trial (p<0.001). There was a significant influence of boar (p<0.001) and sperm concentration (p<0.01) on motility, while acrosome integrity was affected only by boar (p<0.001). Breed of the boars and weight of the ejaculate did not influence the dependent variables. longtime storage; sperm concentration.

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Section: Methodsmentioning

confidence: 99%

Influence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Boar Semen Stored for Five Days

Kommisrud¹,

Paulenz

Sehested³

et al. 2002

Acta Vet Scand

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“…Simultaneously, smears from each sample were prepared for evaluation of acrosome integrity using a dichromatic stain (Spermac®) as described by Oettlé (1986) and Paulenz et al (1995b). The smears were examined under oil immersion (1000 ×) using a bright field microscope.…”

Section: Methodsmentioning

confidence: 99%

Effect of Long‐Term Storage at Different Temperatures on the Quality of Liquid Boar Semen

Paulenz

Kommisrud²,

Hofmo³

2000

Reprod Domestic Animals

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ContentsIn this study the e}ect of long!term storage of liquid boar semen at di}erent temperatures on motility\ acrosome integrity and pH was investigated[ Additionally\ individual variation in sperm tolerance to storage at 09>C were examined[ Beltsville Thawing Solution!diluted AI doses from 05 randomly chosen Norwegian Landrace AI boars with proven fertility were split into subsamples and stored at 14\ 19\ 04 and 09>C\ respectively[ After 9\ 13\ 37\ 61 and 85 h of storage\ sperm motility\ acrosome integrity and pH were determined[ After 85 h\ the initial per! centage of motile sperm "66[7)# was signi_cantly reduced to 41[1\ 47[7\ 49[8 and 31[7) by storage at 14\ 19\ 04 and 09>C\ respectively[ After an identical period of time\ the percentage of acrosome intact sperm "84[7)# at time 9 became signi_cantly reduced to 80[2\ 80[2\ 70[4 and 57[2) by storage at 14\ 19\ 04 and 09>C\ respectively[ The initial pH "6[10# decreased sig! ni_cantly to 5[85 and 6[95 after 85 h storage at 14 and 19>C\ and increased not signi_cantly to 6[14 for storage at 04>C and signi_cantly to 6[18 at 09>C[ In conclusion\ the results from this study show that\ according to the variables studied\ 19>C is the least harmful of the four temperatures tested for the long! term liquid storage of boar semen[ Furthermore\ remarkable di}erences in the individual resistance of boar semen to long! term storage at 09>C were observed[

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“…Obtained data showed that the highest losses in spermatozoa survival happens during freezing and thawing, with consequent decrease of progressive motility rate and cell membrane damages, according several authors (Paulenz et al, 1995;Huang et al, 1999;Ericsson, 2000;Holt, 2000).…”

Section: Resultsmentioning

confidence: 84%

Resistance to controlled thermal stress and tolerance to sperm cryopreservation of two groups of boars

Stankovic¹,

Hristov²,

Petrujkić

et al. 2012

BIOTEC ANIM HUSB

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The aim of this study was to find out is it possible to presume success of boars sperm cryopreservation, based on controlled exposure to heat stress and to investigate in vitro quality parameters of frozen/thawed sperm of previously selected 6 boars with good results (group 1) and 6 boars (group 2) with bad results on thermo resistance test (TT). In this investigation, ejaculates of 12 chosen of 75 AI boars (Landrace, Large White, Pietrain and Durock breeds) from SVC Velika Plana (Serbia) were used. Tolerance to heat stress was performed by Schaetz (1963) method. Ejaculates were extended with Androhep plus (1:1) and kept during 60 minutes on 41 o C. In cryopreservation of boars semen Westendorf et al. (1975) method, modified by Bwanga et al. (1990). Very significant changes of progressive motility rate were observed after spermatozoa exposure to controlled thermal stress. High correlation coefficient of progressive motility rate of both groups of boars, but higher in group 2 for progressive motility rate after thawing and after heat stress treatment was established. Average progressive motility rate of all 12 boars did not differ before and after thawing. Presence of boars of all breeds represented in the SVC (Large White, Landrace, Duroc and Pietrain) in both quality groups confirms the assumption that suitability for cryopreservation of sperm is individual trait. According data analysis, sperm deep freezing success requires previous selection for potential donors, which have to be consider standard quality parameters testing and controlled stress exposure tests.

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The Use of a Dichromatic Stain Method (Spermac®) for Determining Changes in the Acrosomal Integrity of Boar Semen during Cryopreservation

Cited by 9 publications

References 13 publications

Influence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Boar Semen Stored for Five Days

Influence of Boar and Semen Parameters on Motility and Acrosome Integrity in Liquid Boar Semen Stored for Five Days

Effect of Long‐Term Storage at Different Temperatures on the Quality of Liquid Boar Semen

Resistance to controlled thermal stress and tolerance to sperm cryopreservation of two groups of boars

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