The usage of mutant genotypes in tomato selection for specific traits at the institute for vegetable crops

Zdravković, Jasmina; Marković, Z.; Zdravković, Milan; Mijatovic, M.; Pavlović, Nenad

doi:10.2298/gensr1203701z

Cited by 6 publications

(3 citation statements)

References 8 publications

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“…Because of their reduced genome, phytoplasmas lack most essential metabolic pathways [28–30]. Although phytoplasma has been tried to cultivate in vitro [31, 32], their culture in complex artificial media is still difficult. Phytoplasma is transmitted by primarily phloem-sucking insects such as leafhoppers (Cicadellidae, e.g.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of three histone marks and gene expression in Paulownia fortunei with phytoplasma infection

Yan

Fan

2019

BMC Genomics

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BackgroundPaulownia withes’-broom (PaWB) disease caused by phytoplasma is a serious infectious disease for Paulownia. However, the underlying molecular pathogenesis is not fully understood. Recent studies have demonstrated that histone modifications could play a role in plant defense responses to pathogens. But there is still no available genome-wide histone modification data in non-model ligneous species infected with phytoplasma.ResultsHere, we provided the first genome-wide profiles of three histone marks (H3K4me3, H3K36me3 and H3K9ac) in Paulownia fortunei under phytoplasma stress by using chromatin immunoprecipitation sequencing (ChIP-Seq). We found that H3K4me3, H3K36me3 and H3K9ac were mainly enriched in the genic regions in P. fortunei with (PFI) and without (PF) phytoplasma infection. ChIP-Seq analysis revealed 1738, 986, and 2577 genes were differentially modified by H3K4me3, H3K36me3 and H3K9ac marks in PFI under phytoplasma infection, respectively. The functional analysis of these genes suggested that most of them were mainly involved in metabolic pathways, biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, plant-pathogen interaction and plant hormone signal transduction. In addition, the combinational analysis of ChIP-Seq and RNA-Seq showed that differential histone methylation and acetylation only affected a small subset of phytoplasma-responsive genes.ConclusionsTaken together, this is the first report of integrated analysis of histone modifications and gene expression involved in Paulownia-phytoplasma interaction. Our results will provide the valuable resources for the mechanism studies of gene regulation in non-model plants upon pathogens attack.Electronic supplementary materialThe online version of this article (10.1186/s12864-019-5609-1) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of three histone marks and gene expression in Paulownia fortunei with phytoplasma infection

Yan

Fan

2019

BMC Genomics

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“…At the difference of j-2 , plants containing j-2 ^in do not show elongated peduncle and slow fruit ripening, and hence may be also suitable for breeding programs. The gene causing the j-2 ^in mutation remains unknown but together with J-2 could be the key for tomato resistance to drought because it has been shown that the absence of AZ in flower peduncle increases water transport and thus allows to reduce watering during production 31 .…”

Section: Discussionmentioning

confidence: 99%

Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato

Roldán

Périlleux

Morin

et al. 2017

Sci Rep

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Abscission is the mechanism by which plants disconnect unfertilized flowers, ripe fruits, senescent or diseased organs from the plant. In tomato, pedicel abscission is an important agronomic factor that controls yield and post-harvest fruit quality. Two non-allelic mutations, jointless (j) and jointless-2 (j-2), controlling pedicel abscission zone formation have been documented but only j-2 has been extensively used in breeding. J was shown to encode a MADS-box protein. Using a combination of physical mapping and gene expression analysis we identified a positional candidate, Solyc12g038510, associated with j-2 phenotype. Targeted knockout of Solyc12g038510, using CRISPR/Cas9 system, validated our hypothesis. Solyc12g038510 encodes the MADS-box protein SlMBP21. Molecular analysis of j-2 natural variation revealed two independent loss-of-function mutants. The first results of an insertion of a Rider retrotransposable element. The second results of a stop codon mutation that leads to a truncated protein form. To bring new insights into the role of J and J-2 in abscission zone formation, we phenotyped the single and the double mutants and the engineered alleles. We showed that J is epistatic to J-2 and that the branched inflorescences and the leafy sepals observed in accessions harboring j-2 alleles are likely the consequences of linkage drags.

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“…Phytophthora infestans (Mont.) de Bary, an oomycete causing late blight, is the primary agent of tomato yield losses, [1][2][3] and it can cause severe crop damage. Additionally, in locations where sexual reproduction occurs, oospores of the genus Phytophthora can survive for months or years in the absence of living hosts.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative response of tomato genotypes to late blight infection

Medić-Pap

Danojević

Prvulović

et al. 2020

JSCS

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Wild species are widely used as potential sources of resistance of tomato to late blight (LB) (causal agent Phytophthora infestans). The biochemical response of wild and cultivated tomato genotypes with different levels of resistance to P. infestans was assessed through the total phenolic and flavonoid content and antioxidative capacity. In total, six genotypes were included in the research -three cultivated tomato varieties and three wild species. The wild genotypes Solanum pimpinellifolium S 220 and Solanum habrochaites had a significantly lower infection rate compared to the other tested genotypes. After disease assessment on the leaves, biochemical analyses were performed. Grouping of the wild accessions according to principal component analysis (PCA) analysis indicated similar reaction to LB infection. Furthermore, late blight trait is closer to cultivated genotypes. Although the phenolics and flavonoids have high importance in the reaction of tomato plants to late blight infection, these traits are not closely related to wild species and the disease. According to this study, the antioxidative tests that indicate a response of wild species to late blight infection are total antioxidant activity (TAA), ferric-reducing antioxidant power (FRAP) and radical cation scavenging activity (ABTS).

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The usage of mutant genotypes in tomato selection for specific traits at the institute for vegetable crops

Cited by 6 publications

References 8 publications

Genome-wide analysis of three histone marks and gene expression in Paulownia fortunei with phytoplasma infection

Genome-wide analysis of three histone marks and gene expression in Paulownia fortunei with phytoplasma infection

Natural and induced loss of function mutations in SlMBP21 MADS-box gene led to jointless-2 phenotype in tomato

Antioxidative response of tomato genotypes to late blight infection

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