THE UPTAKE OF COPPER BY PLANTS OF <i>MIMULUS GUTTATUS</i> DIFFERING IN GENOTYPE PRIMARILY AT A SINGLE MAJOR COPPER TOLERANCE LOCUS

Macnair, Mark R.

doi:10.1111/j.1469-8137.1981.tb01749.x

Cited by 37 publications

(21 citation statements)

References 15 publications

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“…The altered phosphate (and arsenate) uptake system may be an adaptation of the plants to the low nutrient status of the mine soils from which the plants were growing. It is only by making comparisons between genotypes that differ solely in their tolerance to metal excesses, such as tolerant mutants screened from otherwise non-tolerant populations or by breeding, can conclusions be drawn about the physiological nature of tolerance (Macnair, 1981 ;Macnair & Watkins, 1983 ;Baker & Walker, 1990).…”

Section: Arsenate Uptake and Translocation In The Presence Of Phosphatementioning

confidence: 99%

Uptake, accumulation and translocation of arsenate in arsenate‐tolerant and non‐tolerant Holcus lanatus L.

1991

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SUMMARYUptake, translocation and accumulation of arsenate was determined in arsenate-tolerant and non-tolerant genotypes of Holcus lanatus L. Over a 6 h period of growth in 0-05 mol m"^ arsenate, non-tolerant genotypes accumulated arsenate to a much greater extent than tolerant plants. Tolerant plants transported a much greater proportion of As to their shoots compared with non-tolerant plants. Phosphate at a concentration of either 0-OS or 0-5 mol m"" decreased arsenate uptake in both tolerant and non-tolerant genotypes. When arsenate uptake was determined over 3 d at the same arsenate concentration, non-tolerant plants grown in the presence of 0 and 0-05 mol m"' phosphate died, while those growing in 0-5 mol m"' phosphate survived and continued to take up arsenate. At all three phosphate levels tolerant plants survived. Over the 3 d period transport of arsenate to the shoots decreased. With increasing phosphate levels in solution transport of arsenate to the shoots increased in tolerant plants. The results from these experiments are discussed in terms of strategies for metal tolerance in this species.

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Section: Arsenate Uptake and Translocation In The Presence Of Phosphatementioning

confidence: 99%

Uptake, accumulation and translocation of arsenate in arsenate‐tolerant and non‐tolerant Holcus lanatus L.

1991

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“…1 can be large, up to 50 cm in height and producing large numbers of seeds, whereas plants growing on the tailings off the stream are mostly small, producing many fewer seeds (see Macnair et aL, 1989 Macnair, 1981a;Strange & Macnair, 1991 for details). These lines consist of homozygous tolerant and non-tolerant plants which differ essentially only at the major gene for tolerance identified by Macnair (1983).…”

Section: Introductionmentioning

confidence: 99%

“…Macnair (1983) showed that there was a single major gene giving copper tolerance, and has by repeated backcrossing inserted this gene into a non-tolerance genetic background to produce more or less isogenic lines (Macnair, 1981a;Macnair & Watkins, 1983;Strange & Macnair, 1991). The gene is expressed in the male gametophyte and some selection may act at this stage (Searcy & Mulcahy, 1985;Searcy & Macnair, 1990).…”

Section: Introductionmentioning

confidence: 99%

Heritability and distribution of variation in degree of copper tolerance in Mimulus guttatus at Copperopolis, California

1993

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The variation in degree of copper tolerance, its heritability and relationship with copper contamination of the soil and geographical location of sample, was studied on samples of seed collected from a large abandoned copper mine at Copperopolis, California. The realized heritability of variation in degree of tolerance at a high level of copper, 2 1ug cm , is shown to be about 0.40. The F1s of crosses between an upward and downward selection line and a non-tolerant line give intermediate values for tolerance. All seed samples taken from contaminated soils, and a number from populations downstream of the mine, have 100 per cent tolerant plants. Samples taken from upstream of the mine, and many populations in the vicinity, have a proportion of tolerant plants. The degree of tolerance of the samples is more variable, with rather steep dines for this character within the mine. The variation in degree of tolerance is related more to the location of the sample than to the absolute amount of copper contamination of the soil. Populations along the stream running through the centre of the mine wastes are more tolerant than populations taken from the edge of the wastes, which are more tolerant than populations collected from uncontaminated soils near the mine. The difference in steepness of the dines for the two characters, tolerance and degree of tolerance, suggests that selection against tolerance (the 'cost' of tolerance) acts more on degree of tolerance than on tolerance per se. These results are discussed in relation to the concept of geno stasis.

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“…The generation used is indicated in parentheses in the text. Macnair, 1981 andStrange &Macnair, 1991 for details). These plants do not possess the major copper tolerance gene, which is present in all the selection lines.…”

Section: Provenance Of Plant Materialsmentioning

confidence: 99%

Can an increased copper requirement in copper‐tolerant Mimulus guttatus explain the cost of tolerance? II. Reproductive phase

1998

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Circumstantial evidence suggests that plants that have evolved metal tolerance are at a disadvantage on normal soil, i.e. there is a cost of tolerance. One hypothesis for the cause of this cost is that individuals have a greater requirement for copper, and so suffer micronutrient deficiency on normal soils, as a result of a reduced uptake, distribution and\or utilization of copper. We provided highly and less copper-tolerant plants of Mimulus guttatus Fischer ex DC. (the common monkey flower) with sub-optimal copper, and demonstrated the importance of copper as an essential micronutrient during the reproductive phase, both in the production of viable pollen and in seed set. We also looked at the effect of sub-optimal copper supply on the growth of the microgametophyte, and the efficiency with which seed was set. No evidence was found that highly tolerant plants have an increased copper requirement during the reproductive phase. This is in agreement with earlier work on Mimulus guttatus, which investigated the copper requirement of highly tolerant plants during vegetative growth and found that any differences in copper requirement were small. The ' metal requirement hypothesis ' is, therefore, not the sole explanation for the cost of copper tolerance in M. guttatus.

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THE UPTAKE OF COPPER BY PLANTS OF MIMULUS GUTTATUS DIFFERING IN GENOTYPE PRIMARILY AT A SINGLE MAJOR COPPER TOLERANCE LOCUS

Cited by 37 publications

References 15 publications

Uptake, accumulation and translocation of arsenate in arsenate‐tolerant and non‐tolerant Holcus lanatus L.

Uptake, accumulation and translocation of arsenate in arsenate‐tolerant and non‐tolerant Holcus lanatus L.

Heritability and distribution of variation in degree of copper tolerance in Mimulus guttatus at Copperopolis, California

Can an increased copper requirement in copper‐tolerant Mimulus guttatus explain the cost of tolerance? II. Reproductive phase

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