The uptake, metabolism, and biological half-life of benzo[a]pyrene administered by force-feeding in sea bass (Dicentrarchus labrax)

Lemaire, Philippe; Lemaire-Gony, S.; Berhaut, J.; Lafaurie, M.

doi:10.1016/0147-6513(92)90062-8

Cited by 25 publications

(13 citation statements)

References 19 publications

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“…This is in accordance with previous studies of fish exposed intragastrically to BaP (James et al 1991;McElroy et al 1991;Lemaire et al 1992) and Phen (Solbakken et al 1979;Solbakken and Palmork 1980;Solbakken et al 1982Solbakken et al , 1983. Furthermore, our results suggest that a larger fraction of Phen was subjected to systemic distribution than BaP.…”

Section: Discussionsupporting

confidence: 82%

“…The low levels of radioactivity detected in the livers of specimens exposed to BaP compared to Phen could indicate a more efficient hepatic metabolism of BaP than Phen. Low levels of radioactivity in the liver have also been reported in sea bass (Dicentrarchus labras) force fed BaP (Lemaire et al 1992). A more efficient metabolism of BaP compared to Phen is also supported by Niimi and Palazzo (1986) who showed that the half-life (t 1/2 ) of BaP was significantly shorter (\2 days) in rainbow trout (Oncorhynchus mykiss) intragastrically fed BaP in comparison to Phen (t 1/2 = 9 days).…”

Section: Discussionsupporting

confidence: 52%

“…Biliary excretion of PAHs is well documented in fish (e.g. Solbakken and Palmork 1981;James et al 1991;Lemaire et al 1992;Van Veld et al 1997), and PAH metabolites in fish bile are widely used as biomarker of PAH exposure (Beyer et al 2010). PAHs taken up via the diet will primarily be transported to the liver via the hepatic portal vein and undergo first path metabolism, where their metabolites will be secreted to the bile (Varanasi et al 1989;Kleinow et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Although of special importance for lipophilic contaminants, the dietary route of exposure has not been studied to the same extent as the waterborne exposure. Furthermore, studies have focused on a few model compounds, particularly BaP (James et al 1991(James et al , 1996Lemaire et al 1992;Ingebrigtsen et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Comparative absorption and tissue distribution of 14C-benzo(a)pyrene and 14C-phenanthrene in the polar cod (Boreogadus saida) following oral administration

Bakke¹,

Nahrgang

Ingebrigtsen³

2015

Polar Biol

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The Arctic is an important sink for organic pollutants such as polycyclic aromatic hydrocarbons (PAHs) long-range transported from industrial regions. With the retreat of sea ice and increasing anthropogenic activities such as the oil and gas industries, local sources of PAHs are expected to increase both through operational and accidental discharges. There is a need to increase our knowledge concerning the uptake and distribution of organic pollutants, in particular PAHs, to evaluate the risk these toxic compounds may represent for Arctic species. The absorption and tissue distribution of 14 C-benzo(a)-pyrene (BaP) and 14 C-phenanthrene (Phen) were studied in the polar cod (Boreogadus saida), a key Arctic species. After a single oral dose of BaP (1.15 ± 0.36 mg/kg fish) or Phen (0.40 ± 0.12 mg/kg fish), corresponding to 0.12 ± 0.03 mCi/kg fish, the tissue distribution was followed through 30 days by means of whole-body autoradiography and liquid scintillation counting of liver and bile. For both compounds, radiolabeling was mainly present in the bile and the intestines throughout the study period. Phen-derived radioactivity, however, appeared to be more systemically distributed compared to BaP. Furthermore, a far higher amount of irreversibly bound BaPderived radioactivity was present in the intestinal mucosa compared to Phen, indicating a more extensive formation of reactive intermediates from the former compared with the latter. Liquid scintillation counting confirmed that radioactivity was present in the liver at all time points for both groups although the levels were low in the BaP group. These results strongly indicated that both compounds and/ or their metabolites undergo enterohepatic circulation.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative absorption and tissue distribution of 14C-benzo(a)pyrene and 14C-phenanthrene in the polar cod (Boreogadus saida) following oral administration

Bakke¹,

Nahrgang

Ingebrigtsen³

2015

Polar Biol

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“…Two potential reasons for this may be a poor extraction efficiency for BaP from food due to the poor quality of the food or a low assimilation efficiency across the gut membrane specifically for BaP. Significant uptake of BaP after an oral exposure has been documented for fish in a number of studies, [5,7,43]. The second explanation also merits consideration since it has been suggested that the assimilation efficiency for BaP is very low in rainbow trout [40,42].…”

Section: Uptake Of Benzo[a]pyrene In a Detritivorous Fishmentioning

confidence: 99%

THE ROLE OF WATER VENTILATION AND SEDIMENT INGESTION IN THE UPTAKE OF BENZO[a]PYRENE IN GIZZARD SHAD (DOROSOMA CEPEDIANUM)

Kolok¹,

Huckins²,

Petty³

et al. 1996

Environ Toxicol Chem

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The objective of this study was to determine whether sediment ingestion or water ventilation was the primary route of uptake for benzo[a]pyrene (BaP) in the gizzard shad (Dorosoma cepedianum), a detritivorous fish. Two experiments were conducted in which fish were exposed to sediments spiked with 1 μg/g BaP. In the first experiment, fish were prevented from feeding by esophagus ligation. In the second experiment, 20 nonligated fish and 30 ligated fish were added to the aquarium. The nonligated fish roiled the water as they fed. Fish were collected 4, 8, 15, and 22 d after the experiments began. Gizzard shad metabolize BaP; therefore, the concentrations of BaP equivalents (parent BaP plus metabolite) were determined. Concentrations of BaP equivalents were significantly greater in the ligated fish in experiment 2 relative to those in experiment 1. In contrast, the concentration of BaP equivalents in the ligated fish in experiment 2 was not significantly different than that in the nonligated fish. Our results suggest that ventilation of turbid water may be a significant source of BaP for gizzard shad. Sediment ingestion, however, does not appear to significantly influence the total body concentration of BaP equivalents in gizzard shad.

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Uptake and depuration of polycyclic aromatic hydrocarbons from sediment by the zebrafish (Brachydanio Rerio)

Djomo

Garrigues

Narbonne

1996

Enviro Toxic and Chemistry

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Zebrafish (Brachydanio rerio) were exposed for 30 d to individual 14C‐labeled polycyclic aromatic hydrocarbons (PAHs) (anthracene, phenanthrene, pyrene, or benzo[a]pyrene) adsorbed on sediment. The uptake and depuration rate constants were measured. Rate of radioactivity release was measured under both contaminated (experiment 1) and clean (experiment 2) conditions. The results confirm that uptake and depuration rate constants were strongly related to physicochemical properties. However, the uptake from the water compartment was rapidly decreased in our microcosm system (–83% for anthracene and phenanthrene, –71% for pyrene, and –59% for benzo[a]pyrene within the first 2 d). For depuration in clean water, the half‐lives of radioactivity loss from whole body were 63, 97, 115, and 138 h for anthracene, phenanthrene, pyrene, and benzo[a]pyrene, respectively, and were positively correlated with log Kow. In experiment 1 after 10 d, the remaining PAH concentrations were only about 16% of the maximum concentrations measured at time 24 h for anthracene, 12% for phenanthrene, 30% for pyrene, and 46% for benzo[a]pyrene. Kow appears to be a determining factor for partitioning constants for sorption to sediment, uptake, and release in aquatic organisms.

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The uptake, metabolism, and biological half-life of benzo[a]pyrene administered by force-feeding in sea bass (Dicentrarchus labrax)

Cited by 25 publications

References 19 publications

Comparative absorption and tissue distribution of 14C-benzo(a)pyrene and 14C-phenanthrene in the polar cod (Boreogadus saida) following oral administration

Comparative absorption and tissue distribution of 14C-benzo(a)pyrene and 14C-phenanthrene in the polar cod (Boreogadus saida) following oral administration

THE ROLE OF WATER VENTILATION AND SEDIMENT INGESTION IN THE UPTAKE OF BENZO[a]PYRENE IN GIZZARD SHAD (DOROSOMA CEPEDIANUM)

Uptake and depuration of polycyclic aromatic hydrocarbons from sediment by the zebrafish (Brachydanio Rerio)

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