The uptake and retrograde transport of horseradish peroxidase-polylysine conjugate by ligated postganglionic sympathetic nervesin vitro

Anderson, Patrick N.; Medlen, A. R.; Mitchell, John M.; Mayor, D.

doi:10.1007/bf01181742

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…This assumption of different ways/ kinetics of axonal transport is supported by data showing that, for example, modifications of the horseradish peroxidase lead to different trafficking kinetics. 27 We therefore propose that early after ONC a window is open for retrograde (nonclassical) axonal transport, but this window is not only temporally limited but also restricted to selected chemical/particular entities. Another possible explanation of retrograde RGCs labeling in a 2-day period is that the posttraumatic "transport window" is exclusively open for the fast transport mechanism.…”

Section: Discussionmentioning

confidence: 99%

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Prilloff

Henrich‐Noack

Sabel

2012

Invest. Ophthalmol. Vis. Sci.

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After damage many surviving RGCs lose their axon transport, but after approximately 3 weeks, this transport recovers again, a sign of intrinsic axon repair. Contrary to the prediction, axon transport recovery is not associated with better cell survival but rather with a second wave of cell death. Thus, the accelerated cell death associated with recovery of axon transport suggests the existence of a late retrograde cell death signal.

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Section: Discussionmentioning

confidence: 99%

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Prilloff

Henrich‐Noack

Sabel

2012

Invest. Ophthalmol. Vis. Sci.

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“…Two different lengths of PDL were used: a low molar mass PDL (15000−30000 g/mol for the HBr salt) and a high molar mass PDL (150000−300000 g/mol for the HBr salt). α-Polylysine bioconjugates reported in literature include conjugates with nucleic acids, as well as with enzymes, − for achieving enhanced cellular uptake.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Catalytically Active, Covalent α-Polylysine−Enzyme Conjugates via UV/Vis-Quantifiable Bis-aryl Hydrazone Bond Formation

et al. 2010

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Covalent UV/vis-quantifiable bis-aryl hydrazone bond formation was investigated for the preparation of conjugates between α-poly-d-lysine (PDL) and either α-chymotrypsin (α-CT) or horseradish peroxidase (HRP). PDL and the enzymes were first modified via free amino groups with the linking reagents succinimidyl 6-hydrazinonicotinate acetone hydrazone (S-HyNic, at pH 7.6) and succinimidyl 4-formylbenzoate (S-4FB, at pH 7.2), respectively. The modified PDL and enzymes were then conjugated at pH 4.7, whereby polymer chains carrying several enzymes were obtained. Kinetics of the bis-aryl hydrazone bond formation was investigated spectrophotometrically at 354 nm. Retention of the enzymatic activity after conjugate formation was confirmed by using the substrates N-succinimidyl-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide (for α-CT) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS, for HRP). Thus, not only a mild and efficient preparation and convenient quantification of a conjugate between the polycationic α-polylysine and enzymes could be shown, but also the complete preservation of the enzymatic activity.

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“…Thus the organelles in which HRP undergoes orthograde transport appear identical to those in which the enzyme is transported retrogradely (Anderson et al, 1981). However, it is not surprising that the same protein should be transported by similar organelles regardless of direction.…”

Section: Discussionmentioning

confidence: 95%

A morphological study of the uptake and orthograde axoplasmic transport of horseradish peroxidase by damaged autonomic neuronsin vitro

et al. 1981

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A preparation comprising the guinea-pig inferior mesenteric ganglion (IMG) and ligated hypogastric nerves was maintained in vitro in a twin-chamber apparatus. Horseradish peroxidase (HRP), added to the ganglion compartment, was taken up via coated pinocytotic vesicles into the neuronal perikarya, and subsequently accumulated in many polymorphic cytoplasmic organelles, but it did not enter the Golgi complex. After 24 h incubation, HRP was also localized in membrane-bounded organelles in the nonmyelinated axons of the hypogastric nerves in the ligated nerve compartment. HRP-labelled organelles accumulated in swollen nonmyelinated axons immediately proximal to the ligation along with other organelles known to undergo fast axoplasmic transport. It is suggested that HRP taken up by the neuronal perikarya subsequently underwent fast axoplasmic transport without passing through the Golgi complex. The organelles in which HRP was transported in an orthograde direction were very similar to those in which the enzyme is known to be transported retrogradely in the same neuronal system. The advantages of this in vitro system for studying the orthograde transport of exogenous protein are discussed.

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The uptake and retrograde transport of horseradish peroxidase-polylysine conjugate by ligated postganglionic sympathetic nervesin vitro

Cited by 4 publications

References 12 publications

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Recovery of Axonal Transport after Partial Optic Nerve Damage Is Associated with Secondary Retinal Ganglion Cell Death In Vivo

Preparation of Catalytically Active, Covalent α-Polylysine−Enzyme Conjugates via UV/Vis-Quantifiable Bis-aryl Hydrazone Bond Formation

A morphological study of the uptake and orthograde axoplasmic transport of horseradish peroxidase by damaged autonomic neuronsin vitro

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