The University

deBoer, Fredrik

doi:10.1017/9781316691663.014

Cited by 3 publications

(3 citation statements)

References 14 publications

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“…Since total activity depends on the catalyst concentration as well as specific activity, reducing the available catalyst concentration can further increase selection pressure. In practice, intracellular protein concentrations can be lowered in a variety of ways, for example, by the use of low copy plasmids,101 weak promoters102, and inefficient ribosome binding sites 103…”

Section: Genetic Selection Of Novel Chorismate Mutasesmentioning

confidence: 99%

Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines

Gurovic

Lanza

Adánez

et al. 2010

Phytotherapy Research

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Natural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC(50) values of 1.98 ± 0.12 µm and 3.52 ± 0.14 µm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (ΔΨm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of ΔΨm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment.

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Section: Genetic Selection Of Novel Chorismate Mutasesmentioning

confidence: 99%

Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines

Gurovic

Lanza

Adánez

et al. 2010

Phytotherapy Research

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“…[5,100] Techniques such as error-prone PCR can be used to mutagenize a parent gene. [103] Treatment of this pool with DNaseI gives rise to a set of random fragments.…”

Section: Augmenting Weak Enzyme Activitymentioning

confidence: 99%

Investigating and Engineering Enzymes by Genetic Selection

Taylor

Kast

Hilvert

2001

Angew. Chem. Int. Ed.

186

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“…In der Praxis kann die intrazelluläre Proteinkonzentration auf vielfältige Art und Weise, z. B. durch Verwendung von Plasmiden mit geringer Kopienzahl,101 schwachen Promotoren102 und ineffizienten Ribosomenbindungsstellen,103 verringert werden.…”

Section: Genetische Selektion Neuartiger Chorismat‐mutasenunclassified

Genetische Selektion - eine Strategie zur Untersuchung und Herstellung von Enzymen

2001

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Natürliche Enzyme sind über Jahrmillionen durch den graduellen Prozess der darwinistischen Evolution entstanden. Die Grundelemente der Evolution – Mutation, Selektion und Vervielfältigung – können auch im Labor eingesetzt werden, um Proteinkatalysatoren in entsprechend kürzeren Zeiträumen herzustellen und zu charakterisieren. Genetische In‐vivo‐Selektionsstrategien ermöglichen eine erschöpfende Analyse von Proteinbibliotheken mit 1010 unterschiedlichen Mitgliedern; und mit In‐vitro‐Methoden lassen sich sogar noch größere Ensembles studieren. Folglich können evolutionäre Ansätze statistisch wertvolle Einsichten in komplexe und oftmals subtile Wechselwirkungen geben, die die Proteinfaltung, die Struktur und den katalytischen Mechanismus beeinflussen. Solche Methoden werden zunehmend auch als Hilfsmittel beim Proteindesign eingesetzt, da sie den Zugang zu neuen Proteinen mit maßgeschneiderten katalytischen Aktivitäten und Selektivitäten eröffnen.

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The University

Cited by 3 publications

References 14 publications

Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines

Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines

Investigating and Engineering Enzymes by Genetic Selection

Genetische Selektion - eine Strategie zur Untersuchung und Herstellung von Enzymen

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