The unique structure of the Paramecium caudatum hemoglobin gene: The presence of one intron in the middle of the coding region

Yamauchi, Kiyoshi; Ochiai, Takehiko; Usuki, Itaru

doi:10.1016/0167-4781(92)90142-m

Cited by 38 publications

(23 citation statements)

References 38 publications

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“…Sequence analysis of the heterogeneous macronuclear DNA fragments of the intergenic region between the hb and nap-1 genes The hb gene is located in a 15 kbp BamHI fragment or 3-5 kbp EcoRI fragments obtained from stock YC genomic DNA (Yamauchi et al, 1992b). Partial genomic libraries in λEMBL3 and λgt11 were prepared and screened with a 0.4 kbp cDNA encoding the major hb gene (Yamauchi et al, 1992a).…”

Section: Resultsmentioning

confidence: 99%

“…EcoRI sub-fragments of this DNA fragment were recloned into pUC19. A 3.2 kbp EcoRI fragment containing the hb gene was selected by Southern blotting (Yamauchi et al, 1992b). The hb cDNA did not hybridize to the other EcoRI sub-fragments.…”

Section: Resultsmentioning

confidence: 99%

“…Cell lines and cultivation The P. caudatum stock (syngen 3, stock YC) previously analyzed (Yamauchi et al, 1992b) was used here. P. caudatum cells were cultured at 20-25°C in fresh lettuce juice diluted with Dryl's physiological salt solution (Dryl, 1959), which was modified by the substitution of KH 2 PO 4 for NaH 2 PO 4 , pH 7.0, and inoculated with Klebsiella pneumoniae the day before use (Mikami, 1987).…”

Section: Methodsmentioning

confidence: 99%

“…Total genomic DNA was extracted as described previously (Yamauchi et al, 1992b). Partial genomic libraries of P. caudatum total genomic DNA were constructed using λEMBL3 and λgt11.…”

Section: Isolation and Characterization Of Genomic Clonesmentioning

confidence: 99%

“…Southern blotting of the P. caudatum hemoglobin gene (hb) revealed at least three major different sized fragments in the macronuclear genome (Yamauchi et al, 1992b). This result raised several possibilities: (1) a hb gene family exists whose members are structurally and functionally related (Usuki et al, 1989), and are distributed on distinct chromosomes, like the mammalian α and β hb genes (Hardison, 1998); (2) several copies of the same or highly related hb gene, produced recently by gene duplication generating a tandem repeated gene cluster similar to mammalian and invertebrate hb gene clusters (Efstratiadis et al, 1980;Antoine and Niessing, 1984), exist; (3) IESs at the hb gene or its flanking regions are excised alternatively or differentially to generate heterogeneity during macronuclear development (Austerberry et al, 1984;Herrick et al, 1987); (4) IIDSs are imprecisely excised, leading either to chromosome fragmentation followed by the addition of new telomeres or to the rejoining of flanking sequences (Caron, 1992;Le Mouël et al, 2003); (5) homologous or non-homologous recombinations occur among the hundreds of copies of macronuclear DNA during or after macronuclear development (Deak and Doerder, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes

et al. 2010

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The intergenic region between the hemoglobin (hb) and nucleosome assembly protein-1 (nap-1) genes in the Paramecium caudatum macronuclear genome was previously found to be heterogeneously composed. Cloning of this intergenic region from the macronuclear genomic DNA identified four unique DNA fragments of different sizes. Sequencing of the cloned fragments revealed extreme heterogeneity and characteristics of both internal eliminated sequence (IES) and imprecise internal deletion sequences (IIDSs) in the intergenic region. Missing sequences were an AT-rich and direct repeats existed in their boundaries. Southern blotting of the total genomic DNA and polymerase chain reaction (PCR) of the total genomic DNAs indicated that there exist a dozen DNA fragments of different sizes in this intergenic region. It is likely that the heterogeneity found in the P. caudatum macronuclear genome results from the variable removal of an intergenic region.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Total genomic DNA was extracted as described previously (Yamauchi et al, 1992b). Partial genomic libraries of P. caudatum total genomic DNA were constructed using λEMBL3 and λgt11.…”

Section: Isolation and Characterization Of Genomic Clonesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes

et al. 2010

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The enigmatic oxygen‐avid hemoglobin of Ascaris

Goldberg

1995

BioEssays

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The parasitic nematode Ascaris lives in the low-oxygen intestinal folds of over one billion people world-wide. The worm has an octameric hemoglobin that binds oxygen four orders of magnitude more tightly than does human hemoglobin. Our studies have focused on elucidating the molecular mechanism of oxygen avidity, the basis of multimerization and the function of this remarkable molecule. We now believe that we understand a fair amount about the molecular interactions that result in enhanced avidity, have some preliminary ideas on octamer formation, and have some hypotheses about possible function. Along the way we have stumbled into the disciplines of intron evolution, sterol biosynthesis and oxygen-regulated gene expression.

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Sequence and evolution of the gene for the monomeric globin I and its linkage to genes coding for dimeric globins in the insect Chironomus thummi

et al. 1995

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We isolated genomic clones containing sequences encoding globins I and IA from a Chironomus thummi thummi genomic library. Three clones contain globin IA (ctt-1A) genes, while one contains a globin I (ctt-1) gene. The coding regions of the four genes are identical except for the single base substitution accounting for the globin I/IA polymorphism. The noncoding DNA flanking the coding region is more than 98% similar, confirming a previous hypothesis that the globin ctt-1 and ctt-1A genes are alleles. Hemoglobins I and IA are monomeric in the insect hemolymph. Earlier in situ hybridization studies suggested that monomeric and dimeric globin genes are clustered at different chromosomal loci. In situ hybridization of ctt-1 DNA to polytene salivary gland chromosomes places the ctt-1 gene on the same band as genes for the dimeric globins II beta and VIIB, forcing revision of the earlier hypothesis that genes for monomeric and dimeric globin genes are at different loci. The evolution of the ctt-1 and ctt-1A alleles and of the two globin gene loci are discussed.

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The unique structure of the Paramecium caudatum hemoglobin gene: The presence of one intron in the middle of the coding region

Cited by 38 publications

References 38 publications

Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes

Extreme heterogeneous composition of the Paramecium caudatum macronuclear genomic DNA between hemoglobin and nucleosome assembly protein-1 genes

The enigmatic oxygen‐avid hemoglobin of Ascaris

Sequence and evolution of the gene for the monomeric globin I and its linkage to genes coding for dimeric globins in the insect Chironomus thummi

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