The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus
            <i>Phlebovirus</i>

Matsuno, Keita; Kajihara, Masahiro; Nao, Naganori; Mori-Kajihara, Akina; Muramatsu, Mieko; Qiu, Yongjin; Torii, Shiho; Igarashi, Manabu; Kasajima, Nodoka; Mizuma, Keita; Yoshii, Kentaro; Sawa, Hirofumi; Sugimoto, Chihiro; Takada, Ayato; Ebihara, Hideki

doi:10.1128/msphere.00239-18

Cited by 44 publications

(43 citation statements)

References 43 publications

(43 reference statements)

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“…It is noteworthy that our phylogenies recover the paraphyly of tick-borne phleboviruses, with the tick-borne Mukawa virus being more closely related to sandfly-borne phleboviruses than to other tick-borne phleboviruses in all three trees (Figure 4-6). This is all in agreement with the conclusion of (Matsuno et al 2018) according to which the ancestor of sandfly-borne phleboviruses was tick-borne. Furthermore, in our RNA-dependent RNA-polymerase tree, this conclusion is further supported by the position of the Bhanjavirus group of tick-borne phleboviruses, which falls sister to the sandflyborne + Mukawa phlebovirus clade (bootstrap = 96).…”

Section: Phylogenetic Position Of the Bunya-like Brown Spot Virussupporting

confidence: 91%

“…contigs with the corresponding proteins from a diversity of viruses belonging to the Bunyavirales order, including tick and sandfly-borne phleboviruses, phasiviruses, tenuiviruses, goukoviruses and a number of closely related unclassified viruses. Our sampling of bunya-like viruses to construct these alignments was inspired from (Xin et al 2017;Matsuno et al 2018;Harvey et al 2019). Alignments at the amino-acid level and removal of ambiguous regions were performed by hand.…”

Section: Phylogenetic Analysesmentioning

confidence: 99%

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A new bunya-like virus associated with mass mortality of white-clawed crayfish in the wild

et al. 2019

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We report the discovery of a new enveloped, spherical virus belonging to the Phenuiviridae family of negative ssRNA viruses associated with a massive outbreak in a French population of the endangered white-clawed crayfish Austropotamobius pallipes. We call this virus Bunya-like Brown Spot Virus (BBSV) and characterize it using transmission electronic microscopy, genome sequencing and clinical signs. Infected specimens show discolored brown spots on the cuticle. Using RNA-seq data we assembled a partial sequence for the L, M and S genome segments of BBSV. Phylogenetic analyses using all three segments show this virus is closely related to the Wenling crustacean virus 7 (China) and to a bunya-like virus found in feces of the European otter. Our survey of the mass mortality event indicates the virus is less virulent than the crayfish plague caused by Aphanomyces astaci. Overall, the discovery of BBSV provides a new important asset to monitor A. pallipes populations.

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Section: Phylogenetic Position Of the Bunya-like Brown Spot Virussupporting

confidence: 91%

Section: Phylogenetic Analysesmentioning

confidence: 99%

A new bunya-like virus associated with mass mortality of white-clawed crayfish in the wild

et al. 2019

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“…However, viral diversity in ticks from South America is largely unknown (24). In recent years the emergence of new TBPV such as SFTSV and HRTV in Asia and North America, respectively, present a significant public health threat (4). In the present study, TBPV sequences detected in Colombian ticks collected form domestic animals were similar to Lihan tick virus detected in China in R. microplus ticks (23) A limitation of the present study is the short fragment (less than 200 bp) of the L segment used for phylogenetic analysis.…”

Section: Discussionmentioning

confidence: 99%

First report of Lihan Tick virus (Phlebovirus, Phenuiviridae) in ticks, Colombia

López¹,

Miranda²,

Mattar³

et al. 2020

Preprint

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Background: Tick-borne phenuivirus (TBPVs) comprise human and animal viruses that can cause a variety of clinical syndromes ranging from self-limiting febrile illness to fatal haemorrhagic fevers. Objective: Detect Phlebovirus (Family Phenuiviridae) in ticks collected from domestic animals in Córdoba, Colombia. Methods: We collected 2,365 ticks from domestic animals in three municipalities of the Department of Cordoba, Colombia in 2016. Ticks were identified and pooled by species for RNA extraction. A nested real-time PCR with specific primers for Phlebovirus and a specific probe for Heartland virus (HRTV) formerly a Phlebovirus, now a Banyangvirus were performed. Also, a conventional nested PCR, with the same specific primers was used to detect other Phleboviruses , with positive reactions indicated by an amplified cDNA fragment of approximately 244 bp determined by gel electrophoresis. These bands were gel-purified and sequenced by the Sanger method. Results: Using real-time RT-PCR, no positive results for HRTV were found. However, using conventional nested PCR 2.2% (5/229 pools) yielded a product of 244 bp. One positive sample was detected in a pool of Dermacentor nitens ticks collected from a horse, and the four remaining positive pools were from Rhipicephalus microplus collected from cattle. The five positive nucleotide sequences had identities of 93 to 96% compared to a section of the L-segment of Lihan Tick virus , a Phlebovirus originally detected in R. microplus ticks in China. The strongest identity (96 -99%) was with Lihan Tick virus detected in R. microplus ticks from Brazil. Conclusions: This is the first report of viral detection in ticks in Colombia. We detected a Colombian strain of Lihan Tick virus . We recommend expanding the sampling area and carrying out more eco-epidemiological studies related to epidemiological surveillance of viruses on ticks in Colombia.

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“…They can cause a variety of clinical syndromes ranging from a brief, self-limiting febrile illness, to retinitis, encephalitis, meningoencephalitis and fatal haemorrhagic fever [3]. The genus Phlebovirus belongs to the family Phenuiviridae, order Bunyavirales, [4]. The genus includes 10 species with around 58 viruses and several that have not yet been categorized [5].…”

Section: Introductionmentioning

confidence: 99%

First report of Lihan Tick virus (Phlebovirus, Phenuiviridae) in ticks, Colombia

López

Miranda

Máttar

et al. 2020

Virol J

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Background: Tick-borne phenuivirus (TBPVs) comprise human and animal viruses that can cause a variety of clinical syndromes ranging from self-limiting febrile illness to fatal haemorrhagic fevers. Objective: Detect Phlebovirus (Family Phenuiviridae) in ticks collected from domestic animals in Córdoba, Colombia. Methods: We collected 2365 ticks from domestic animals in three municipalities of the Department of Cordoba, Colombia in 2016. Ticks were identified and pooled by species for RNA extraction. A nested real-time PCR with specific primers for Phlebovirus and a specific probe for Heartland virus (HRTV) formerly a Phlebovirus, now a Banyangvirus were performed. Also, a conventional nested PCR, with the same specific primers was used to detect other Phleboviruses, with positive reactions indicated by an amplified cDNA fragment of approximately 244 bp determined by gel electrophoresis. These bands were gel-purified and sequenced by the Sanger method. Results: Using real-time RT-PCR, no positive results for HRTV were found. However, using conventional nested PCR 2.2% (5/229 pools) yielded a product of 244 bp. One positive sample was detected in a pool of Dermacentor nitens ticks collected from a horse, and the four remaining positive pools were from Rhipicephalus microplus collected from cattle. The five positive nucleotide sequences had identities of 93 to 96% compared to a section of the Lsegment of Lihan Tick virus, a Phlebovirus originally detected in R. microplus ticks in China. The strongest identity (96-99%) was with Lihan Tick virus detected in R. microplus ticks from Brazil. Conclusions: This is the first report of viral detection in ticks in Colombia. We detected a Colombian strain of Lihan Tick virus. We recommend expanding the sampling area and carrying out more eco-epidemiological studies related to epidemiological surveillance of viruses on ticks in Colombia.

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The Unique Phylogenetic Position of a Novel Tick-Borne Phlebovirus Ensures an Ixodid Origin of the Genus Phlebovirus

Cited by 44 publications

References 43 publications

A new bunya-like virus associated with mass mortality of white-clawed crayfish in the wild

A new bunya-like virus associated with mass mortality of white-clawed crayfish in the wild

First report of Lihan Tick virus (Phlebovirus, Phenuiviridae) in ticks, Colombia

First report of Lihan Tick virus (Phlebovirus, Phenuiviridae) in ticks, Colombia

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