The Unique Photophysical Properties of the Peridinin-Chlorophyll-a-Protein

Carbonera, Donatella; Valentin, Marilena Di; Spezia, Riccardo; Mezzetti, Alberto

doi:10.2174/1389203715666140327111139

Cited by 37 publications

(38 citation statements)

References 136 publications

(230 reference statements)

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“…These pigments seemed to be pure enough to use the apex as a reference (Retention time and λ max ) in Table 2 . These kinds of spectra are not very common but provide a consistent signal [ 51 ]. Carotenoids are involved in chlorophyll photoprotection.…”

Section: Discussionmentioning

confidence: 99%

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

et al. 2017

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Phytoplankton, with an estimated 30 000 to 1 000 000 species clustered in 12 phyla, presents a high taxonomic and ecophysiological diversity, reflected by the complex distribution of pigments among the different algal classes. High performance liquid chromatography is the gold standard method for qualitative and quantitative analysis of phytoplankton pigments in seawater and culture samples, but only a few pigments can be used as robust chemotaxonomic markers. A major challenge is thus to identify new ones, characteristic of a strain, species, class or taxon that cannot be currently identified on the basis of its pigment signature. Using an optimized extraction process coupled to a HPLC de-replication strategy, we examined the pigment composition of 37 microalgae strains, representative of the broad taxonomic diversity of marine and freshwater species (excluding cyanobacteria). For each species, the major pigments already described were unambiguously identified. We also observed the presence of several minor unidentified pigments in each chromatogram. The global analysis of pigment compositions revealed a total of 124 pigments, including 98 pigments or derivatives unidentified using the standards. Absorption spectra indicated that 35 corresponded to chlorophyll/porphyrin derivatives, 57 to carotenoids and six to derivatives having both spectral signatures. Sixty-one of these unidentified or new carotenoids and porphyrin derivatives were characteristic of particular strains or species, indicating their possible use as highly specific chemotaxonomic markers capable of identifying one strain out of the 37 selected. We developed a graphical analysis using Gephi software to give a clear representation of pigment communities among the various phytoplankton strains, and to reveal strain-characteristic and shared pigments. This made it possible to reconstruct the taxonomic evolution of microalgae classes, on the basis of the conservation, loss, and/or appearance of pigments.

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Section: Discussionmentioning

confidence: 99%

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

et al. 2017

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“…In fact, the early publication of PCP high-resolution X-ray structure 1 revealed that this water-soluble protein has a pseudo-two-fold symmetry and binds eight peridinins (Pers) clustered around two Chl molecules. The detailed structural information, together with the remarkable efficiency for excitation energy transfer (EET) from Car donors to Chl acceptors (~90%) 2 – 5 , have inspired intense research among the biophysical community 6 – 12 . While the properties of Cars as leading actors in photoprotection 13 and non-photochemical quenching 14 have been the object of several in-depth investigations in the last decades 15 – 17 , the mechanistic and dynamic details of the excitation energy flow from Cars to Chls are still not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Coherence in carotenoid-to-chlorophyll energy transfer

et al. 2018

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The subtle details of the mechanism of energy flow from carotenoids to chlorophylls in biological light-harvesting complexes are still not fully understood, especially in the ultrafast regime. Here we focus on the antenna complex peridinin–chlorophyll a–protein (PCP), known for its remarkable efficiency of excitation energy transfer from carotenoids—peridinins—to chlorophylls. PCP solutions are studied by means of 2D electronic spectroscopy in different experimental conditions. Together with a global kinetic analysis and multiscale quantum chemical calculations, these data allow us to comprehensively address the contribution of the potential pathways of energy flow in PCP. These data support dominant energy transfer from peridinin S2 to chlorophyll Qy state via an ultrafast coherent mechanism. The coherent superposition of the two states is functional to drive population to the final acceptor state, adding an important piece of information in the quest for connections between coherent phenomena and biological functions.

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“…The light-harvesting peridinin-chlorophyll-protein (PCP) from the dinoflagellate Amphidinium carterae [20] in its native form is a trimer, where each of the monomers comprises eight peridinin molecules and two Chls a embedded in a protein scaffold [20,[32][33][34]. As shown in Figure 2b, the absorption spectrum of PCP (black) features a band in the range from~400 to 550 nm due to peridinins, while Chl a molecules absorb light in the Soret band (with a maximum around 440 nm) and in the Q y region from 600 to 670 nm.…”

Section: Photosynthetic Complexesmentioning

confidence: 99%

Silver Island Film for Enhancing Light Harvesting in Natural Photosynthetic Proteins

Kowalska

Szalkowski

Sulowska

et al. 2020

IJMS

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The effects of combining naturally evolved photosynthetic pigment–protein complexes with inorganic functional materials, especially plasmonically active metallic nanostructures, have been a widely studied topic in the last few decades. Besides other applications, it seems to be reasonable using such hybrid systems for designing future biomimetic solar cells. In this paper, we describe selected results that point out to various aspects of the interactions between photosynthetic complexes and plasmonic excitations in Silver Island Films (SIFs). In addition to simple light-harvesting complexes, like peridinin-chlorophyll-protein (PCP) or the Fenna–Matthews–Olson (FMO) complex, we also discuss the properties of large, photosynthetic reaction centers (RCs) and Photosystem I (PSI)—both prokaryotic PSI core complexes and eukaryotic PSI supercomplexes with attached antenna clusters (PSI-LHCI)—deposited on SIF substrates.

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The Unique Photophysical Properties of the Peridinin-Chlorophyll-a-Protein

Cited by 37 publications

References 136 publications

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

Community analysis of pigment patterns from 37 microalgae strains reveals new carotenoids and porphyrins characteristic of distinct strains and taxonomic groups

Coherence in carotenoid-to-chlorophyll energy transfer

Silver Island Film for Enhancing Light Harvesting in Natural Photosynthetic Proteins

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