The Unique Evolutionary Distribution of Eukaryotic Elongation Factor 3

Mateyak, Maria K.; Sasikumar, Arjun N.; Dunaway, Stephen; Kinzy, Terri Goss

doi:10.1007/978-3-319-39468-8_14

Cited by 2 publications

(4 citation statements)

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“…While originally designated as a fungal-specific elongation factor, recent bioinformatic analysis identified eEF3-like proteins in several lower eukaryotic species [ 14 , 15 ]. The most significant regions of conservation between these proteins and S .…”

Section: Resultsmentioning

confidence: 99%

“…Bioinformatic analysis of existing genome databases has identified eEF3 orthologues in at least 18 different non-fungal species, including multiple green algae [ 14 ]. Since molecular genetic techniques have not been developed for many of these organisms, complementation of the loss of S .…”

Section: Discussionmentioning

confidence: 99%

“…Recent bioinformatic analysis identified potential eEF3 orthologues in multiple non-fungal, lower eukaryotic species [ 14 , 15 ]. These eEF3-like protein sequences are as similar to S .…”

Section: Introductionmentioning

confidence: 99%

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Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans

et al. 2018

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In most eukaryotic organisms, translation elongation requires two highly conserved elongation factors eEF1A and eEF2. Fungal systems are unique in requiring a third factor, the eukaryotic Elongation Factor 3 (eEF3). For decades, eEF3, a ribosome-dependent ATPase, was considered “fungal-specific”, however, recent bioinformatics analysis indicates it may be more widely distributed among other unicellular eukaryotes. In order to determine whether divergent eEF3-like proteins from other eukaryotic organisms can provide the essential functions of eEF3 in budding yeast, the eEF3-like proteins from Schizosaccharomyes pombe and an oomycete, Phytophthora infestans, were cloned and expressed in Saccharomyces cerevisiae. Plasmid shuffling experiments showed that both S. pombe and P. infestans eEF3 can support the growth of S. cerevisiae in the absence of endogenous budding yeast eEF3. Consistent with its ability to provide the essential functions of eEF3, P. infestans eEF3 possessed ribosome-dependent ATPase activity. Yeast cells expressing P. infestans eEF3 displayed reduced protein synthesis due to defects in translation elongation/termination. Identification of eEF3 in divergent species will advance understanding of its function and the ribosome specific determinants that lead to its requirement as well as contribute to the identification of functional domains of eEF3 for potential drug discovery.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans

et al. 2018

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“…An example of genes showing interesting nsSNPs is eRF1, which binds the ribosome A site and recognizes all three stop codons in eukaryotes [76]. When the elongating ribosome encounters a termination codon, this release factor terminates translation and releases the polypeptide from the ribosome, and ribosomal subunits are recycled to participate in a new round of translation [77]. This gene is related to several protein formation and it was recently found to be essential (together with ESP1) in protein-accumulation mechanisms in rice, providing a genetic target for improving relevant agronomic traits [78].…”

Section: Discussionmentioning

confidence: 99%

EST–SNP Study of Olea europaea L. Uncovers Functional Polymorphisms between Cultivated and Wild Olives

Mariotti

Belaj

Rosa

et al. 2020

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Background: The species Olea europaea includes cultivated varieties (subsp. europaea var. europaea), wild plants (subsp. europaea var. sylvestris), and five other subspecies spread over almost all continents. Single nucleotide polymorphisms in the expressed sequence tag able to underline intra-species differentiation are not yet identified, beyond a few plastidial markers. Methods: In the present work, more than 1000 transcript-specific SNP markers obtained by the genotyping of 260 individuals were studied. These genotypes included cultivated, oleasters, and samples of subspecies guanchica, and were analyzed in silico, in order to identify polymorphisms on key genes distinguishing different Olea europaea forms. Results: Phylogeny inference and principal coordinate analysis allowed to detect two distinct clusters, clearly separating wilds and guanchica samples from cultivated olives, meanwhile the structure analysis made possible to differentiate these three groups. Sequences carrying the polymorphisms that distinguished wild and cultivated olives were analyzed and annotated, allowing to identify 124 candidate genes that have a functional role in flower development, stress response, or involvement in important metabolic pathways. Signatures of selection that occurred during olive domestication, were detected and reported. Conclusion: This deep EST–SNP analysis provided important information on the genetic and genomic diversity of the olive complex, opening new opportunities to detect gene polymorphisms with potential functional and evolutionary roles, and to apply them in genomics-assisted breeding, highlighting the importance of olive germplasm conservation.

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The Unique Evolutionary Distribution of Eukaryotic Elongation Factor 3

Cited by 2 publications

References 40 publications

Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans

Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans

EST–SNP Study of Olea europaea L. Uncovers Functional Polymorphisms between Cultivated and Wild Olives

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