ABSTRACT:Unintended consequences of Electronic Health Records (EHRs) represent undesired effects on individuals or systems which may contradict initial goals and impact patient care. The purpose of this study was to determine the extent to which a new quantitative measure called the Carrington-Gephart Unintended Consequences of Electronic Health Record Questionnaire (CG-UCE-Q) was valid and reliable. Then it was used to describe acute care nurses' experience with unintended consequences of EHRs and relate them to the professional practice environment. Acceptable content validity was achieved over 2 rounds of surveys with nursing informatics experts (N=5). Then acute care nurses (N=144) were recruited locally and nationally to complete the survey and describe the frequency with which they encounter unintended consequences in daily work. Principal components analysis with oblique rotation was applied to evaluate construct validity. Correlational analysis with measures of the professional practice environment and workarounds were used to evaluate convergent validity. Testretest reliability was measured in the local sample (N=68). Explanation for 63% of the variance across 6 subscales (patient safety, system design, workload issues, workarounds, technology barriers and sociotechnical impact), supporting construct validity. Relationships were significant between subscales for EHR related threats to patient safety and low autonomy/leadership (p<0.01), poor communication about patients (p<0.01) and low control over practice (p< 0.01). Most frequent sources of unintended consequences were increased workload, interruptions that shifted tasks from the computer, altered workflow, and the need to duplicate data entry. Convergent validity of the CG-UCE-Q was moderately supported with both the context and processes of workarounds with strong relationships identified for when nurses perceived a block and altered processes to work around it to subscales in the CG-UCE-Q for EHR system design (p< 0.01) and technological barriers (p<0.01).