SYNOPSIS Westergren sedimentation rates were done on 603 healthy blood donors. The results showed an increase in the normal values with age. The normal values found in this series were higher than those generally accepted as normal.The commonly accepted normal values for the Westergren method of determining the sedimentation rate (E.S.R.) are 3-5 mm. in the first hour for males and 7-12 mm. for females (Whitby and Britton, 1963). During routine blood testing we found a number of apparently normal persons whose E.S.R. exceeded these ranges, and in order to assess the significance of this finding, we undertook a survey of the E.S.R. in healthy blood donors. The results showed that the normal E.S.R. is dependent on age as well as sex.
METHODSWe used Dawson's (1960) been withdrawn by a vacuum method. Through the intravenous set which had been used for the donation a further 5 ml. of blood was aspirated into an evacuated 5 ml. bijou bottle containing 5 mg. of sequestrene (E.D.T.A.). The bijou bottles were left standing at room temperature for one to three hours and then mixed on a rotary mixer for a minimum of two minutes. Exactly 2 ml. of blood from each sample was then mixed in another bottle with exactly 0 5 ml. of 3 8 % sodium citrate solution, the same rotary mixer being again used for at least two minutes. One standard Westergren pipette was filled from each sample by drawing blood above the zero mark and allowing it to run back to avoid the 'reflux' effect (Alexander, 1953). The tubes were suspended for one hour and read, the reading being corrected for temperature (Manley, 1957). Room temperatures varied from 20 to 24°C.
RESULTSA total of 603 unselected blood donors was tested in two series. The first consisted of 182 males and 114 females. These were divided into five age groups: 18-30, 31-40, 41-50, 51-60, and over 60 years. The mean value and standard deviation were calculated for each age group and showed a steady increase with age. In order to check this result a second series of 213 males and 94 females was tested, and similar figures were obtained for the means and standard