The underlying mechanisms of anti-hepatitis B effects of formula Le-Cao-Shi and its single herbs by network pharmacology and gut microbiota analysis

Zhao, Qing; Song, Shu-Yue; Zhang, Yuqi; Ren, Xia; Zhang, Peng; Li, Xin; Fu, Xiaojian; Wang, Chang‐Yun

doi:10.1016/j.biopha.2022.112692

Cited by 6 publications

(5 citation statements)

References 59 publications

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“…Compounds in the three single herbs of LCS were collected from the Chemical Database of the Academy of Sciences ( http://www.organchem.csdb.cn/scdb/default.asp ), and manually supplemented through text mining ( Yue et al, 2017 ; Zhao et al, 2022 ). Their chemical structures were obtained from the Chemical Book database ( http://www.chemicalbook.com ), NCBI PubChem database ( http://www.ncbi.nlm.nih.gov/pccompound ), and the literature, and drawn by using Chemdraw software ( https://www.perkinelmer.com.cn/category/chemdraw , version 3.7.2).…”

Section: Methodsmentioning

confidence: 99%

“…The screening thresholds were set as OB ≥ 30%, Caco-2 ≥ −0.14, and DL ≥ 0.1 ( Li et al, 2016 ; Yue et al, 2017 ). Compounds containing glycosyl groups were further deglycosylated and expressed as compound_qt ( Zhao et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Targets of candidate compounds were searched in the Drug Bank database, Similarity Ensemble Approach (SEA) database, STITCH database, and a reverse pharmacophore-based screening platform, Pharm Mapper database ( Knox et al, 2011 ; Kuhn et al, 2014 ; Liu et al, 2015 ; Wang et al, 2017 ; Xu et al, 2018 ; Zhao et al, 2022 ). These predicted targets were chosen by the rank of matching score and then input into the Therapeutic Target Database (TTD), Online Mendelian Inheritance in Man (OMIM) database, Drug Bank database, and PharmGKB database ( Altman, 2007 ; Wishart et al, 2008 ; Jiang et al, 2015 ; Liu et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

“…Its radix has been used as a remedy to treat hepatitis and rheumatism ( State Administration of Traditional Chinese Medicine, 1999b ; Xin et al, 2017 ). C. tricuspidata , the alternative herb of C. cochinchinensis , is difficult to obtain due to its relatively low availability ( Zhao et al, 2022 ) . Although LCS has long been used as a prescription to treat liver injuries, its underlying mechanisms remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation

et al. 2022

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Le-Cao-Shi (LCS) has long been used as a folk traditional Chinese medicine formula against liver injuries, whereas its pharmacological mechanisms remain elusive. Our study aims to investigate the underlying mechanism of LCS in treating liver injuries via integrated network pharmacology, metabonomics, and experimental validation. By network pharmacology, 57 compounds were screened as candidate compounds based on ADME parameters from the LCS compound bank (213 compounds collected from the literature of three single herbs). According to online compound–target databases, the aforementioned candidate compounds were predicted to target 87 potential targets related to liver injuries. More than 15 pathways connected with these potential targets were considered vital pathways in collectively modulating liver injuries, which were found to be relevant to cancer, xenobiotic metabolism by cytochrome P450 enzymes, bile secretion, inflammation, and antioxidation. Metabonomics analysis by using the supernatant of the rat liver homogenate with UPLC-Q-TOF/MS demonstrated that 18 potential biomarkers could be regulated by LCS, which was closely related to linoleic acid metabolism, glutathione metabolism, cysteine and methionine metabolism, and glycerophospholipid metabolism pathways. Linoleic acid metabolism and glutathione metabolism pathways were two key common pathways in both network pharmacology and metabonomics analysis. In ELISA experiments with the CCl4-induced rat liver injury model, LCS was found to significantly reduce the levels of inflammatory parameters, decrease liver malondialdehyde (MDA) levels, and enhance the activities of hepatic antioxidant enzymes, which validated that LCS could inhibit liver injuries through anti-inflammatory property and by suppressing lipid peroxidation and improving the antioxidant defense system. Our work could provide new insights into the underlying pharmacological mechanisms of LCS against liver injuries, which is beneficial for its further investigation and modernization.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation

et al. 2022

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“…Third-generation microbial diversity was based on the PacBio sequencing platform, and the marker gene was sequenced by single molecule real-time sequencing (SMRT Cell). The species composition of each sample was revealed by filtering, clustering or denoising the circular consensus sequence, and species annotation and abundance analysis as previously described [34]. The following analyses were carried out: annotation and taxonomy analysis of species, significant difference analysis, and diversity analysis (alpha and beta diversity).…”

Section: S Rdna Sequencing and Microbiota Analysismentioning

confidence: 99%

Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis

et al. 2022

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Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects. However, the mechanism by which it inhibits colitis is uncertain. This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC). Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days. Pharmacological mechanisms were predicted by network pharmacology. High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition. Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon. Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon. Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria. Network pharmacology revealed that quercetin target genes were enriched in inflammatory and neoplastic processes. Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K). Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.

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Metabolite features of serum and intestinal microbiota response of largemouth bass (Micropterus salmoides) after Aeromonas hydrophila challenge

Jin

Jiang

et al. 2023

Comparative Biochemistry and Physiology Part C: Toxicology &

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The underlying mechanisms of anti-hepatitis B effects of formula Le-Cao-Shi and its single herbs by network pharmacology and gut microbiota analysis

Cited by 6 publications

References 59 publications

Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation

Deciphering the Underlying Mechanisms of Formula Le-Cao-Shi Against Liver Injuries by Integrating Network Pharmacology, Metabonomics, and Experimental Validation

Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis

Metabolite features of serum and intestinal microbiota response of largemouth bass (Micropterus salmoides) after Aeromonas hydrophila challenge

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