The ultrastructure of the hepatic cells in millipedes (Myriapoda, Diplopoda)

Rost‐Roszkowska, Magdalena; Vilímová, Jitka; Tajovský, Karel; Šustr, Vladimír; Sosinka, A.; Kszuk-Jendrysik, M.; Ostróżka, Anna; Kaszuba, F.; Kamińska, Karolina; Marchewka, A.

doi:10.1016/j.jcz.2018.01.006

Cited by 3 publications

(11 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We can state that the autophagy that was observed in the millipedes is a common process that participates in the proper functioning of the midgut epithelium. It has also been considered to be commonly present in the hepatic cells of millipedes (De Godoy & Fontanetti, 2010; Nogarol & Fontanetti, 2011; Rost-Roszkowska et al, 2018 a ) where it participates in glycogen digestion. Autophagy has also been suggested as the survival factor that supplies the energy derived from the digestion of polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

“…In the digestive epithelia of many invertebrates, apoptosis is a natural process which, due to the neutralization of epithelial cells, prevents the tissue against inflammation (Vaidyanathan & Scott, 2006; Maghsoudi et al, 2012; Lipovšek et al, 2018). However, the autophagy does not activate apoptosis and/or necrosis in the hepatic cells of millipedes (Rost-Roszkowska et al, 2018 a ). It would probably be the mechanism that enables the digestion of the reserve material: the cytoplasm of hepatic cells is rich in glycogen granules digested due to autophagy (De Godoy & Fontanetti, 2010; Nogarol & Fontanetti, 2011; Rost-Roszkowska et al, 2018 a ).…”

Section: Discussionmentioning

confidence: 99%

“…However, the autophagy does not activate apoptosis and/or necrosis in the hepatic cells of millipedes (Rost-Roszkowska et al, 2018 a ). It would probably be the mechanism that enables the digestion of the reserve material: the cytoplasm of hepatic cells is rich in glycogen granules digested due to autophagy (De Godoy & Fontanetti, 2010; Nogarol & Fontanetti, 2011; Rost-Roszkowska et al, 2018 a ). In some short-living arthropods, in which regenerative cells were not detected, the autophagy enables to fulfill the digestive cells their functions as long as possible (Rost-Roszkowska et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Apoptosis was observed in the digestive cells of centipedes, but they never occurred in the midgut secretory or regenerative cells (Rost-Roszkowska et al, 2015). As has been mentioned above, the secretory and regenerative cells are closed cell types and their cell membranes do not contact the midgut lumen and food masses (Chajec et al, 2012, 2014; Sosinka et al, 2014; Rost-Roszkowska et al, 2018 a , 2018 b , 2018 c ). The digestive cells are the only cells which contact the nourishments so they are exposed to numerous stressors which can enter the animals' body from the midgut lumen (Vaidyanathan & Scott, 2006; Baton & Ranford-Cartwright, 2007; Franzetti et al, 2012; Wilczek et al, 2014; Lipovšek et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Autophagy and apoptosis have been described as commonly occurring in the midgut epithelium of centipedes (Myriapoda, Chilopoda) (Rost-Roszkowska et al, 2015, 2016), where they have been described in digestive, secretory, and regenerative cells. Among millipedes (Myriapoda, Diplopoda), the autophagy has been described in the cytoplasm of hepatic cells (De Godoy & Fontanetti, 2010; Nogarol & Fontanetti, 2011; Rost-Roszkowska et al, 2018 a ) or fat body cells (Fontanetti et al, 2006). The midgut epithelium of millipedes is lined with a simple epithelium that is composed of three types of cells—digestive, secretory, and regenerative (Hopkin & Read, 1992; Fontanetti & Camargo-Mathias, 1997; Fantazzini et al, 2002; Camargo-Mathias et al, 2004; De Godoy & Fontanetti, 2010; Souza & Fontanetti, 2011; Sosinka et al, 2014; Fontanetti et al, 2015; Rost-Roszkowska et al, 2018 b ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Autophagy and Apoptosis in the Midgut Epithelium of Millipedes

et al. 2019

Self Cite

View full text Add to dashboard Cite

The process of autophagy has been detected in the midgut epithelium of four millipede species: Julus scandinavius, Polyxenus lagurus, Archispirostreptus gigas, and Telodeinopus aoutii. It has been examined using transmission electron microscopy (TEM), which enabled differentiation of cells in the midgut epithelium, and some histochemical methods (light microscope and fluorescence microscope). While autophagy appeared in the cytoplasm of digestive, secretory, and regenerative cells in J. scandinavius and A. gigas, in the two other species, T. aoutii and P. lagurus, it was only detected in the digestive cells. Both types of macroautophagy, the selective and nonselective processes, are described using TEM. Phagophore formation appeared as the first step of autophagy. After its blind ends fusion, the autophagosomes were formed. The autophagosomes fused with lysosomes and were transformed into autolysosomes. As the final step of autophagy, the residual bodies were detected. Autophagic structures can be removed from the midgut epithelium via, e.g., atypical exocytosis. Additionally, in P. lagurus and J. scandinavius, it was observed as the neutralization of pathogens such as Rickettsia-like microorganisms. Autophagy and apoptosis ca be analyzed using TEM, while specific histochemical methods may confirm it.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Autophagy and Apoptosis in the Midgut Epithelium of Millipedes

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

Effect of gluten on the digestive tract and fat body of Telodeinopus aoutii (Diplopoda)

Błaszczyk

Sosinka

Wilczek

et al. 2022

Journal of Morphology

View full text Add to dashboard Cite

Adult specimens or larvae of invertebrates used as food for vertebrates are often maintained close to gluten so they might become vectors for cereal proteins.However, the tissues and internal organs can respond differently in animals with different feeding habits. The midgut epithelium might be a first and sufficient barrier preventing uptake and effects of gluten on the whole body, while the fat body is the main organ that accumulates different xenobiotics. Good models for such research are animals that do not feed on gluten-rich products in their natural environment. The project's goal was to investigate alterations in the midgut epithelium and fat body of the herbivorous millipede Telodeinopus aoutii (Diplopoda) and analyze cell death processes activated by gluten. It enabled us to determine whether changes were intensified or reversed by adaptive mechanisms. Adult specimens were divided into control and experimental animals fed with mushrooms supplemented with gluten and analyzed using transmission electron microscopy, flow cytometry, and confocal microscopy. Two organs were isolated for the qualitative and quantitative analysis: the midgut and the fat body. Our study of the herbivorous T. aoutii which does not naturally feed on gluten containing diet showed that continuous and prolonged gluten feeding activates repair processes that inhibit the processes of cell death (apoptosis and necrosis) and induce an increase in cell viability.

show abstract

Structure of the midgut epithelium in four diplopod species: histology, histochemistry and ultrastructure

Rost‐Roszkowska¹,

Vilímová²,

Tajovský³

et al. 2021

ASP

View full text Add to dashboard Cite

The middle region of the digestive system of millipedes, the midgut, is responsible for all processes connected with digestion, but also takes part in homeostasis maintenance thanks to the ability to activate many mechanisms which neutralize changes occurring at different levels of the animal’s body. Numerous millipede species are treated as bioindicators of the natural environment and they are exposed to different stressors which originate from external environment. To obtain all data on the functioning of midgut of millipedes as the barrier against stressors, it is necessary to have a precise and general description of the midgut epithelium. Members from four millipede orders were selected for the studies: Polydesmus angustus (Polydesmida), Epibolus pulchripes (Spirobolida), Unciger transsilvanicus (Julida) and Glomeris tetrasticha (Glomerida). The structure and ultrastructure of their midgut epithelial cells (the digestive, secretory and regenerative cells) were documented using transmission electron microscopy and histochemical methods. The obtained results have been compared and discussed to previous ones, to present the general and structural organization of the midgut in Diplopoda. Our studies revealed that the ultrastructure of all cells which form the midgut epithelium in millipedes is general for all the species studied up to now and it resembles the cell ultrastructure observed in Chilopoda and Hexapoda, including the digestive, secretory and stem cells.

show abstract

The ultrastructure of the hepatic cells in millipedes (Myriapoda, Diplopoda)

Cited by 3 publications

References 13 publications

Autophagy and Apoptosis in the Midgut Epithelium of Millipedes

Autophagy and Apoptosis in the Midgut Epithelium of Millipedes

Effect of gluten on the digestive tract and fat body of Telodeinopus aoutii (Diplopoda)

Structure of the midgut epithelium in four diplopod species: histology, histochemistry and ultrastructure

Contact Info

Product

Resources

About