The ultrastructure of fiber cells in primate lenses: A model for studying membrane senescence

Kuszak, J.R.; Ennesser, C.A.; Umlas, Joel; Macsai-Kaplan, M.S.; Weinstein, Ronald S.

doi:10.1016/0889-1605(88)90059-6

Cited by 49 publications

(28 citation statements)

References 48 publications

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“…The protuberances found on secondary fibres of older lenses are likely a result of enlarged and irregular lateral interdigitations. These age-related changes in the surface morphology of chicken lens fibres are consistent with those found in other avian species (Kuszak et al, 1980 ;Willekens and Vrensen, 1985), non-primate mammals (Rafferty and Esson, 1974 ;Harding et al, 1976 ;Hollenberg et al, 1976 ;Willekens and Vrensen, 1981 ;Hoyer, 1982 ; and primates (Kuwabara, 1975 ;Willekens and Vrensen, 1982 ;Kuszak et al, 1988 ;Costello et al, 1992 ;Vrensen et al, 1992).…”

Section: Morphologysupporting

confidence: 79%

Effect of Age on the Morphology and Optical Quality of the Avian Crystalline Lens

Priolo

Sivak

Kuszak

1999

Experimental Eye Research

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Section: Morphologysupporting

confidence: 79%

Effect of Age on the Morphology and Optical Quality of the Avian Crystalline Lens

Priolo

Sivak

Kuszak

1999

Experimental Eye Research

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“…After overnight washing in 0 . 2 M sodium cacodylate buffer, the equatorial and A±P axial dimensions were measured under a dissecting microscope (Zeiss, New York, NY, U.S.A.), then dissected as previously described (Kuszak et al, 1988). Brie¯y, a cylindrical core encompassing the lens diameter at birth, was removed using a 6 mm trephine, then the anterior and posterior disks were detached using ®ne forceps.…”

Section: Specimen Preparationmentioning

confidence: 99%

Structural Evidence of Human Nuclear Fiber Compaction as a Function of Ageing and Cataractogenesis

Al-Ghoul

Nordgren

Kuszak

et al. 2001

Experimental Eye Research

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“…The curved crystalline arrays then pair with the protein-poor lipid bilayers to create a new junctional association that is thin and asymmetric, pairing 7 nm thick curved AQP0 arrays with 4-5 nm thick bilayers, to produce junctions about 11 nm thick that have been termed square array junctions (SAJs) (Costello et al, 1985(Costello et al, , 1989Lo and Harding, 1984). In young lenses SAJs dominate the surface topology of nuclear fiber cells producing the undulating polygonal arrays of furrowed membranes (Dickson and Crock, 1972;Kuszak et al, 1988;Vrensen et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural analysis of damage to nuclear fiber cell membranes in advanced age-related cataracts from India

Costello

Johnsen

Metlapally

et al. 2008

Experimental Eye Research

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The primary goal was to characterize the structural alterations that occur at the fiber cell interfaces in nuclei of fully opaque cataracts removed by extracapsular cataract surgery in India. The dark yellow to brunescent nuclei, ages 38-78 years, were probably representative of advanced age-related nuclear cataracts. Thick tissue slices were fixed, en bloc stained and embedded for transmission electron microscopy. Stained thin sections contained well-preserved membranes and junctions, although the complex cellular topology often made it necessary to tilt the grid extensively to visualize the membranes. Damage to the fiber cell membranes was noted in all regions of the nucleus. The most important damage occurred within undulating membrane junctions where the loss of membrane segments was common. These membrane breaks were not sites of fusion as membrane edges were detected and cytoplasm appeared to be in contact with extracellular space, which was enlarged in many regions. Dense deposits of protein-like material were frequently observed within the extracellular space and appeared to be similar to protein in the adjacent cytoplasm. The deposits were often 20-50 nm thick, variable in length and located on specific sites on plasma membranes and between clusters of cells or cell processes. In addition, low density regions were seen within the extracellular space, especially within highly undulating membranes where spaces about 100 nm in diameter were observed. The membrane damage was more extensive and extracellular spaces were larger than in aged transparent donor lenses. Because high and low density regions contribute equally to the fluctuations in refractive index, the changes in density due to the observed damage near membranes are likely to produce significant light scattering based on theoretical analysis. The dimensions of the fluctuations in the range 20-100 nm imply that the scattering is probably similar to that of small particles that would increase high-angle scattering visible in the slit lamp. Such damage to membranes would be expected to contribute to the total opacification of the nucleus as the cataract matures. The main sources of the fluctuations appear to be the degradation of membranes and adjacent cytoplasmic proteins, as well as the redistribution of proteins and fragments.

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The ultrastructure of fiber cells in primate lenses: A model for studying membrane senescence

Cited by 49 publications

References 48 publications

Effect of Age on the Morphology and Optical Quality of the Avian Crystalline Lens

Effect of Age on the Morphology and Optical Quality of the Avian Crystalline Lens

Structural Evidence of Human Nuclear Fiber Compaction as a Function of Ageing and Cataractogenesis

Ultrastructural analysis of damage to nuclear fiber cell membranes in advanced age-related cataracts from India

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