The ultrastructure of erythroblastic islands and reticular cells in mouse bone marrow

Berman, Irwin R.

doi:10.1016/s0022-5320(67)80050-9

Cited by 82 publications

(23 citation statements)

References 18 publications

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“…Micropinocytic veiscles are often found while the occurrence of iron particles in the vesicles is infrequent. These findings agree with the reports of Berman (1967) …”

Section: Ultrastructure Of the Erythroblasts In The Splenic Coloniessupporting

confidence: 94%

Ultrastructure of the erythroblasts in mice splenic colonies.

Miura

Yoshida

Yamaguchi

et al. 1978

Tohoku J. Exp. Med.

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“…Micropinocytic veiscles are often found while the occurrence of iron particles in the vesicles is infrequent. These findings agree with the reports of Berman (1967) …”

Section: Ultrastructure Of the Erythroblasts In The Splenic Coloniessupporting

confidence: 94%

Ultrastructure of the erythroblasts in mice splenic colonies.

Miura

Yoshida

Yamaguchi

et al. 1978

Tohoku J. Exp. Med.

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“…The widespread distribution of macrophages in bone marrow has been noted by a number of investigators in several mammalian species (Bessis, 1958;Ben-Ishay & Yoffey, 1971;Weiss, 1976;Berman, 1976). The resident population is quite distinct from the immature members of the mononuclear phagocyte system present in bone marrow, which comprise monoblasts, promonocytes and monocytes.…”

Section: Resident Macrophages In Haematopoietic Tissuesmentioning

confidence: 90%

Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells

Crocker

Morris

Gordon

1988

Journal of Cell Science

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SummaryImmunocytochemical staining of tissues with the mouse macrophage-specific monoclonal antibody, F4/80, has shown that large numbers of stromal macrophages are present in adult and foetal haematopoietic tissues. Macrophage plasma membrane processes are seen to establish extensive associations with myeloid and erythroid cells in adult bone marrow and with developing erythroblasts in foetal liver, suggestive of local trophic interactions. To explore the nature of these interactions, methods were developed for isolation of resident bone marrow macrophages (RBMM) and foetal liver macrophages (FLM). Following collagenase digestion of bone marrow or foetal liver, clusters were obtained which were composed of one or more central macrophages surrounded by proliferating haematopoietic cells. After attachment of clusters to glass coverslips, adherent macrophages could be stripped free of haematopoietic cells by pipetting in the absence of divalent cations. The purified RBMM, but not FLM, expressed a novel haemagglutinin, which mediated binding, without ingestion, of large numbers of unopsonized sheep erythrocytes by a divalent cation-independent mechanism. In view of the possibility that this sheep erythrocyte receptor (SER) could interact with a homologous ligand on mouse bone marrow cells, its properties were examined. SER was found to be a lectin-like protein which recognized protease-resistant sialylated glycoconjugates on sheep erythrocytes. The expression of SER was restricted to certain stromal tissue macrophages and was low or absent on monocytes and macrophages obtained from serous cavities. High levels of SER could be induced on elicited peritoneal macrophages by cultivation in mouse serum and the induced receptor was found to mediate low-avidity binding of murine bone marrow cells with characteristics indistinguishable from those seen for binding of sheep erythrocytes. However, maximal binding of bone marrow cells to RBMM depended on a distinct, divalent cation-dependent adhesion system. Using erythroblasts as a ligand, FLM were selected to explore the properties and expression of this adhesion receptor, the erythroblast receptor (EbR). Similar to SER, EbR did not mediate ingestion, and was restricted in its expression to foetal and adult stromal tissue macrophages. Unlike SER, EbR activity was not affected by neuraminidase treatment of the ligand and the receptor was not induced on peritoneal macrophages cultured in mouse serum. EbR appears to be a novel cell adhesion receptor because it was unaffected by inhibitors of several previously described cell adhesion molecules, including the fibronectin receptor. Future studies will attempt to explore the functional significance of these two receptors in macrophage-haematopoietic cell interactions.

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“…In this relation, electron microscope studies have demonstrated that macrophages in mouse bone marrow often possess cytoplasmic inclusions, which are characterized by the presence of crystalloids that are needle-like in appearance (BERMAN, 1967;HUDSON, 1968). The crystalloid-containing inclusions have been reported to increase in number with age, although their functional significance is not fully understood (HUDSON, 1969;HUDSON and SHORTLAND, 1974;YANG et al, 1979).…”

Section: Discussionmentioning

confidence: 99%

“…They are considered to be concerned functionally and intimately with hemopoiesis (BESSIS and BRETON-GORIUS, 1962;SEKI and SHIRASAWA, 1965;MARTON, 1975). In mice and rabbits, macrophages in the hemopoietic organ are known to possess not only spherical phagolysosomes but also peculiar long, straight and slender inclusions which, containing needle-like crystalloid structures, are referred to as crystalloid or paracrystalline inclusions (MOORE et al, 1964;BERMAN, 1967;HUDSON, 1968;SIMON and BURKE, 1970;CRICHTON et al, 1980). In mice, as seen in the results, the crystalloid inclusions are found in macrophages of the bone marrow and the splenic red pulp which is hemopoietic-largely erythropoietic in function (BozzINI et al, 1970;SASAKI et al, 1982).…”

Section: Discussionmentioning

confidence: 99%

Crystalloid inclusion-containing macrophages in the bone marrow and red pulp of the mouse, with particular relation to age, sex and hydrocortisone administration: Qualitative and quantitative electron microscopy.

Sasaki¹,

Matsumura²,

Ito³

1983

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary.Cytoplasmic inclusions, particularly crystalloid inclusions of macrophages in mouse bone marrow and splenic red pulp were qualitatively and quantitatively examined by electron microscopy.In mice younger than 40 days of age, macrophages in the bone marrow contain a variety of inclusions, the majority of which are spherical phagolysosomes.In mice older than 60 days, needle-like crystalloid structures occur within the phagolysosomes. The crystalloids first appear at 60 days of age, and increase in amount with age. They exhibit a remarkable increase between 90 and 120 days in females and between 60 and 90 days in males.The crystalloids are significantly more abundant in adult males than in adult females. Macrophages in the splenic red pulp have much more spherical inclusions than marrow macrophages.The crystalloid inclusions are also contained in the red pulp, but they are quite small in amount as compared with those in the marrow. After administration of hydrocortisone, the amount of crystalloids increase significantly in marrow macrophages.The crystalloids in macrophages are discussed with particular interest to their relation to erythropoietic activity in the hemopoietic tissue.

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The ultrastructure of erythroblastic islands and reticular cells in mouse bone marrow

Cited by 82 publications

References 18 publications

Ultrastructure of the erythroblasts in mice splenic colonies.

Ultrastructure of the erythroblasts in mice splenic colonies.

Novel cell surface adhesion receptors involved in interactions between stromal macrophages and haematopoietic cells

Crystalloid inclusion-containing macrophages in the bone marrow and red pulp of the mouse, with particular relation to age, sex and hydrocortisone administration: Qualitative and quantitative electron microscopy.

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