The ultrastructure and reproduction of Amphiamblys capitellides (Microspora, Metchnikovellidae), a parasite of the gregarine Ancora sagittata (Apicomplexa, Lecudinidae), with redescription of the species and comments on the taxonomy

Larsson, J.I. Ronny; Køie, Marianne

doi:10.1016/j.ejop.2006.07.001

Cited by 21 publications

(44 citation statements)

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“…2014). Unusual for microsporidians, the life cycle of metchnikovellids lacks merogonial stages—spores are produced by sporogony, often endogenously inside cysts or spore sacs (Larsson 2000; Larsson and Køie 2006; Sokolova et al. 2013), the size and shape of which serve as a diagnostic trait for classification.…”

Section: Introductionmentioning

confidence: 99%

Genomic survey of a hyperparasitic microsporidianAmphiamblyssp. (Metchnikovellidae)

2016

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Metchnikovellidae are a group of unusual microsporidians that lack some of the defining ultrastructural features characteristic of derived Microsporidia and are thought to be one of their earliest-branching lineages. The basal position of metchnikovellids was never confirmed by molecular phylogeny in published research, and thus far no genomic data for this group were available. In this work, we obtain a partial genome of metchnikovellid Amphiamblys sp. using multiple displacement amplification, next-generation sequencing, and metagenomic binning approaches. The partial genome, which we estimate to be close to 90% complete, displays genome compaction on par with gene-dense microsporidian genomes, but contains an unusual repertoire of unique repeat elements. Phylogenetic analyses of multigene datasets place Amphiamblys sp. as the first branch of the microsporidian lineage following the divergence of a mitochondriate microsporidian Mitosporidium. We find evidence for a mitochondrial remnant presumably functionally equivalent to a mitosome in Amphiamblys sp. and the common enzymatic complement for microsporidian anaerobic metabolism. Comparative genomic analyses identify the conservation of components for clathrin vesicle formation as one of the key features distinguishing the metchnikovellid from its highly derived relatives. The presented data confirm the notion of Metchnikovellidae as a less derived microsporidian group, and provide an additional stepping stone for reconstruction of an evolutionary transition from the early diverging parasitic fungi to derived Microsporidia.

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Section: Introductionmentioning

confidence: 99%

Genomic survey of a hyperparasitic microsporidianAmphiamblyssp. (Metchnikovellidae)

2016

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“…Their affiliation with other groups of protists, Haplosporidia, Chitrids or Plasmodiophorales, was debated (Caullery and Mesnil, 1919) until Vivier proposed the microsporidian nature of metchnikovellids based on similarities in spore ultrastructure and intracellular development (Vivier, 1965). Metchnikovellids lack the standard characters of microsporidia, such as polaroplast and coiled polar filament; this group has unusual general spore morphology, different spore wall structure, and can produce spores endogenously inside cysts (Vivier, 1975) that are also called spore sacs (Larsson, 2000;Larsson and Køie, 2006). Sprague, who first claimed Microsporidia as a phylum, erected the class Rudimicrosporea, restricted exclusively to the order Metchnikovellida, in contrast to the class Microsporea embracing all other microsporidia (Sprague, 1977).…”

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confidence: 99%

Fine structure of Metchnikovella incurvata Caullery and Mesnil 1914 (microsporidia), a hyperparasite of gregarines Polyrhabdina sp. from the polychaete Pygospio elegans

et al. 2013

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Class Rudimicrosporea Sprague 1977, with its single family Metchnikovellidae, comprises hyperparasites of gregarines from the guts of marine invertebrates. Metchnikovellids remain poorly studied in spite of their significance to the evolutionary history of microsporidia; their ultrastructure and life cycles require further investigation. Here we present results of the light- and electron-microscopy study of Metchnikovella incurvata Caulleri and Mesnil 1914, isolated from lecudinid gregarines, parasitizing polychaetes Pygospio elegans in the White Sea littoral zone, and yet described only on the light-microscopic level. The life cycle of this microsporidium includes 2 sporogonies: free (FS) and sac-bound (SBS). In FS, sporonts develop into multinuclear cells (sporogonial plasmodia), which generate sporoblasts and free spores residing in direct contact with the host cytoplasm. Electron microscopy revealed their metchnikovellidean structure: a horseshoe-shaped nucleus, short manubrium perpendicular to the long axis of the spore, and a polar cap in a separate membrane container. Merogony was not observed. The earliest stages of SBS were chains of binucleate cells. They underwent a series of nuclear and cell divisions, produced extracellular envelopes, and split into boomerang-shaped spore sacs, containing up to 16 spores each. Ultrastructure and sizes of sac-bounded spores were similar to those of free-living ones. An amended diagnosis of M. incurvata is provided.

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“…In Chytridiopsis socius, Manier and Ormieres (1968) designated the structures as ''centrosomes". The plaques are also present in the nuclei of Steinhausia (Richards and Sheffield, 1970), and appear again in Amphiamblys capitellides (Larsson and Koie, 2006).…”

Section: Discussionmentioning

confidence: 94%

Budding: A new stage in the development of Chytridiopsis typographi (Zygomycetes: Microsporidia)

Tonka

Weiser

2010

Journal of Invertebrate Pathology

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The ultrastructure and reproduction of Amphiamblys capitellides (Microspora, Metchnikovellidae), a parasite of the gregarine Ancora sagittata (Apicomplexa, Lecudinidae), with redescription of the species and comments on the taxonomy

Cited by 21 publications

References 5 publications

Genomic survey of a hyperparasitic microsporidianAmphiamblyssp. (Metchnikovellidae)

Genomic survey of a hyperparasitic microsporidianAmphiamblyssp. (Metchnikovellidae)

Fine structure of Metchnikovella incurvata Caullery and Mesnil 1914 (microsporidia), a hyperparasite of gregarines Polyrhabdina sp. from the polychaete Pygospio elegans

Budding: A new stage in the development of Chytridiopsis typographi (Zygomycetes: Microsporidia)

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