The ultrastructural alterations in the lens capsule and epithelium in eyes with traumatic white cataract

İnanç, Merve; Tekin, Kemal; Erol, Yasemin Özdamar; Sargon, Mustafa F.; Koç, Mustafa; Budakoglu, Ozlem; Yılmazbaş, Pelin

doi:10.1007/s10792-017-0783-0

Cited by 8 publications

(5 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The clarity of the lens then progressively worsened with the duration of short-wavelength blue light exposure. The process of cataract formation illustrated in this study is consistent with that reported in an earlier study [35]. In this study, the phenotype is a cataract involving lens fiber cells.…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

RETRACTED ARTICLE: Role of short-wavelength blue light in the formation of cataracts and the expression of caspase-1, caspase-11, Gasdermin D in rat lens epithelial cells: insights into a novel pathogenic mechanism of cataracts

et al. 2020

View full text Add to dashboard Cite

Background: With the popularity of blue-rich light-emitting diode (LED)-backlit display devices, our eyes are now exposed to more short-wavelength blue light than they were in the past. The goal of this study was to investigate the pathogenesis of cataracts after short-wavelength light exposure. Methods: Sprague-Dawley (SD) rats were selected and randomly divided into a control group (10 rats each for the 4-, 8-, and 12-week groups) and an experimental group (10 rats each for the 4-, 8-, and 12-week groups). The rats in the experimental group were exposed to a short-wavelength blue LED lamp for 12 h per day. After exposure to the blue LED lamp, the rats were maintained in total darkness for 12 h, after which a 12-h light/dark cycle was resumed. The intensity of the lamp was 3000 lx. At the end of the short-wavelength blue LED lamp exposure (for 4, 8, and 12 weeks), the expression levels of caspase-1, caspase-11 and gasdermin D (GSDMD) were examined in rat lens epithelial cells (LECs) using qRT-PCR and Western blot analyses. An illuminance of 2500 lx was used to study the potential effect of blue LED light on HLE-B3 hLECs in vitro. AC-YVAD-CMK, a caspase-1 inhibitor, was used to confirm the pyroptosis of LECs by flow cytometry. Results: After 6 weeks, cataracts developed in the experimental rats (4/20 eyes). The clarity of the lens gradually worsened with the duration of exposure. Twelve weeks later, all of the rat eyes had developed cataracts. The expression levels of caspase-1, caspase-11 and GSDMD at 4, 8, and 12 weeks were significantly higher in the samples from rats exposed to a short-wavelength blue LED lamp than in the samples from control rats (p<0.05). The proportions of double-positive hLECs were significantly increased in the 5-h and 10-h short-wavelength blue light exposure subgroups compared with the 5-h and 10-h caspase-1 inhibitor subgroups (p < 0.05).

show abstract

Section: Discussionsupporting

confidence: 92%

“…Studies have confirmed that cumulative visible light exposure may accelerate the development of cataracts [ 34 ]. Dysfunction of the LECs may lead to superficial cortical lens fiber edema and mature cataracts [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

RETRACTED ARTICLE: Role of short-wavelength blue light in the formation of cataracts and the expression of caspase-1, caspase-11, Gasdermin D in rat lens epithelial cells: insights into a novel pathogenic mechanism of cataracts

et al. 2020

View full text Add to dashboard Cite

show abstract

“…It acts as an "optical windshield," which is exposed to not only longer wavelength ultraviolet (UV) radiation (300-400 nm) but also the full range of visible light (400-760 nm). When visible light passes through the cornea into the eye, it first comes in contact with the human lens epithelial cells (hLECs), cataract formation determined by this study is consistent with that determined by an earlier study 34 .…”

Section: Discussionsupporting

confidence: 89%

WITHDRAWN: Role of Short-wavelength Blue Light in the Formation of Cataract and Expression of Caspase-1, -11 and Gasdermin D in rat Lens Epithelium Cells: Insight into the Novel Pathogenesis of Cataract.

Wang

Zhang

Sun

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Cataracts have been verified to be associated with a number of risk factors. The sun and artificial light sources, including light-emitting diode (LED) and fluorescent light tubes, are the primary sources of short-wavelength blue light. With the increasing popularity of blue-rich LED-backlit display devices, our eyes are now exposed to more short-wavelength blue light than they were in the past. The goal of this study was to evaluate the role of short-wavelength blue light in the formation of cataract. Additionally, the pathogenesis of cataracts after short-wavelength light exposure was investigated.Methods SD rats were randomly divided into 2 main groups: a control group (10 rats each for the 4-, 8-, and 12-week groups) and an experimental group (10 rats each for the 4-, 8-, and 12-week groups). The rats in the experimental group were exposed to a short-wavelength blue LED lamp for 12 hours per day. After exposure to the blue LED lamp, the rats were maintained in total darkness for 12 hours, after which a 12-hour light/dark cycle was resumed. The intensity of the lamp was 3000 lux. At the end of the short-wavelength blue LED lamp exposure (for 4, 8, and 12 weeks), the expression levels of caspase-1, caspase-11 and gasdermin D (GSDMD) in rat epithelium cells (LECs) were examined in rat epithelial cells (LECs) using qRT-PCR and Western blotting analyses. Results After 6 weeks, cataracts had developed in the experimental rats (4/20 eyes). The clarity of the lens then gradually worsened with the duration of exposure. Twelve weeks later, all of the rat eyes had developed cataracts. Then the expression levels of caspase-1, caspase-11 and GSDMD at 4, 8, and 12 weeks were significantly higher in samples from rats exposed to a short-wavelength blue LED lamp than samples from control rat (p˂0.05). Conclusion The data indicate that pyroptosis play a key role of in cataracts induced by short-wavelength blue light exposure, highlighting caspase-1, caspase-11 and GSDMD as possible therapeutic targets for cataract treatment. This study might provide new insight into the novel pathogenesis of cataracts.

show abstract

“…In this study, we investigated the ultrastructure of aLC and LECs in HMC compared with ARC by TEM, to analyze the possible etiological mechanism of HMC. A lot of efforts have been devoted to explore the electron microscopic architecture of lens capsule since decades ago [6][7][8][9][10][11][12][13][14][15]. However, high-myopic cataracts haven't been involved so far.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of Anterior Lens Capsule and Epithelium in High-Myopic Cataracts

Wang¹,

Wu²,

Cai³

et al. 2021

Preprint

View full text Add to dashboard Cite

BACKGROUND: The mechanism of high-myopic cataract (HMC) is not fully understood yet. The ultrastructure of anterior lens capsule (aLC) and lens epithelial cells (LECs) may provide some clues to this issue.METHODS: Capsulorhexis samples from 10 eyes with HMC and 10 eyes with age-related cataract (ARC) were analyzed for morphologic characteristics using transmission electron microscopy (TEM).RESULTS: Several common pathologic disorders of LECs, including pleomorphic cellular structure, extensive intracellular vacuoles, swollen organelles, degeneration and necrotic lesions, could be observed in both HMC group and ARC group, to a similar extent. Specimens of HMC group disclosed remarkable extracellular spaces, interdigitation of LECs as well as protuberances of aLC towards LECs. CONCLUSIONS: The ultrastructure of aLC and LECs in HMC demonstrated some specific pathologic disorders in comparison with ARC, which might play a role in the formation of cataract in high myopes.

show abstract

The ultrastructural alterations in the lens capsule and epithelium in eyes with traumatic white cataract

Abstract: The ultrastructural findings of our cases support that traumatically induced dysfunction of the lens epithelium may lead to an edema in superficial cortical lens fibers that subsequently undergo degeneration and produce a localized zone of vacuolization.

Cited by 8 publications

References 18 publications

RETRACTED ARTICLE: Role of short-wavelength blue light in the formation of cataracts and the expression of caspase-1, caspase-11, Gasdermin D in rat lens epithelial cells: insights into a novel pathogenic mechanism of cataracts

RETRACTED ARTICLE: Role of short-wavelength blue light in the formation of cataracts and the expression of caspase-1, caspase-11, Gasdermin D in rat lens epithelial cells: insights into a novel pathogenic mechanism of cataracts

WITHDRAWN: Role of Short-wavelength Blue Light in the Formation of Cataract and Expression of Caspase-1, -11 and Gasdermin D in rat Lens Epithelium Cells: Insight into the Novel Pathogenesis of Cataract.

Ultrastructure of Anterior Lens Capsule and Epithelium in High-Myopic Cataracts

Contact Info

Product

Resources

About