The Ultramicroscopic Structure of Enamel and Dentin

Kennedy, James J.; Teuscher, George W.; Fosdick, L.S.

doi:10.14219/jada.archive.1953.0079

Cited by 15 publications

(1 citation statement)

References 10 publications

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“…Since its introduction, scanning electron microscope (SEM) has become a fundamental tool in modern dental research (Bernick et al 1952;Bra¨nnstro¨m et al 1980;Kennedy et al 1952;Yasuzuki and Obata 1955). SEM is used in morphological in vitro studies of specimens such as extracted teeth Lee et al 1997;Somma et al 2008;Van Eldik et al 2004), dental materials (Asgary et al 2009;Baldissara et al 2006;Scotti et al 2007), and dental instruments (Alexandrou et al 2006;Alapati et al 2005;Ounsi et al 2008;Prati et al 2004).…”

Section: Introductionmentioning

confidence: 99%

A systematic method for predetermined scanning electron microscope analysis in dental science

et al. 2010

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Scanning electron microscope evaluation could be criticized if the method adopted to correct for bias is not specified in the study design. Observers can draw conclusions from images unconsciously chosen to best support their research hypotheses, impairing the basic research principle of operator's impartiality. In this study, a systematic observation method has been described and verified for repeatability. The number and the observation points on a certain specimen have been predetermined using a scheme along with observation rules previously established in the research protocol. When our instrument is used at an operating magnification between 500x and 1,000x (corresponding to a frame of 250x190 micro and 120x90 micro, respectively), the method allowed 100% repeatable observation frames, with linear frame errors in finding an observation point of 12.5% in length and 16.8% in height. With modifications to accommodate research objective and statistical requirements, the method could be applied to many SEM observation study.

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