The Ubiquitous Distribution of Late Embryogenesis Abundant Proteins across Cell Compartments in <i>Arabidopsis</i> Offers Tailored Protection against Abiotic Stress

Candat, Adrien; Paszkiewicz, Gaël; Neveu, Martine; Gautier, Romain; Logan, David C.; Avelange‐Macherel, Marie‐Hélène; Macherel, David

doi:10.1105/tpc.114.127316

Cited by 186 publications

(196 citation statements)

References 128 publications

(164 reference statements)

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“…We checked how remodeling of the chondriome during germination affected the distribution of mitochondrial nucleoids. By staining with SYBR Green I in a line expressing mCherry in the mitochondrial matrix (Candat et al, 2014), we were able to both visualize mtDNA and mitochondria in the embryo, from 10 min (A) Images generated using Imaris to track mitochondria in cotyledon cells, maintained throughout the time course in 100 mM ABA, GA 3 , or paclobutrazol, after stratification for 48 h at 4°C and 2 h after transfer to light/21°C. Detected mitochondria were colored green, and tracks are rainbow colored according to speed (no movement is purple, and movement above 0.032 mm/s is red).…”

Section: The Perinuclear Mitochondria Enable Interaction and Redistrimentioning

confidence: 99%

Arabidopsis Seed Mitochondria Are Bioenergetically Active Immediately upon Imbibition and Specialize via Biogenesis in Preparation for Autotrophic Growth

et al. 2017

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Seed germination is a vital developmental transition for production of progeny by sexual reproduction in spermatophytes. Quiescent cells in nondormant dry embryos are reawakened first by imbibition and then by perception of germination triggers. Reanimated tissues enter into a germination program requiring energy for expansion growth. However, germination requires that embryonic tissues develop to support the more energy-demanding processes of cell division and organogenesis of the new seedling. Reactivation of mitochondria to supply the required energy is thus a key process underpinning germination and seedling survival. Using live imaging, we investigated reactivation of mitochondrial bioenergetics and dynamics using Arabidopsis thaliana as a model. Bioenergetic reactivation, visualized by presence of a membrane potential, is immediate upon rehydration. However, reactivation of mitochondrial dynamics only occurs after transfer to germination conditions. Reactivation of mitochondrial bioenergetics is followed by dramatic reorganization of the chondriome (all mitochondrial in a cell, collectively) involving massive fusion and membrane biogenesis to form a perinuclear tubuloreticular structure enabling mixing of previously discrete mitochondrial DNA nucleoids. The end of germination coincides with fragmentation of the chondriome, doubling of mitochondrial number, and heterogeneous redistribution of nucleoids among the mitochondria, generating a population of mitochondria tailored to seedling growth.

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Section: The Perinuclear Mitochondria Enable Interaction and Redistrimentioning

confidence: 99%

Arabidopsis Seed Mitochondria Are Bioenergetically Active Immediately upon Imbibition and Specialize via Biogenesis in Preparation for Autotrophic Growth

et al. 2017

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“…Seedlings of friendly were transformed essentially as described by Candat et al (2014), except that 0.01% (v/v) Silwet L-77 was used instead of Tween 20. Microscopy of transformed seedlings was performed using a Nikon A1 confocal laser-scanning microscope equipped with a 403 water-immersion objective (NA = 0.9) and using 488-and 561-nm excitation for GFP and mRFP1, respectively.…”

Section: Transient Transformation Of Arabidopsis Seedlingsmentioning

confidence: 99%

FRIENDLY Regulates Mitochondrial Distribution, Fusion, and Quality Control in Arabidopsis

et al. 2014

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Mitochondria are defining components of most eukaryotes. However, higher plant mitochondria differ biochemically, morphologically, and dynamically from those in other eukaryotes. FRIENDLY, a member of the CLUSTERED MITOCHONDRIA superfamily, is conserved among eukaryotes and is required for correct distribution of mitochondria within the cell. We sought to understand how disruption of FRIENDLY function in Arabidopsis (Arabidopsis thaliana) leads to mitochondrial clustering and the effects of this aberrant chondriome on cell and whole-plant physiology. We present evidence for a role of FRIENDLY in mediating intermitochondrial association, which is a necessary prelude to mitochondrial fusion. We demonstrate that disruption of mitochondrial association, motility, and chondriome structure in friendly affects mitochondrial quality control and leads to mitochondrial stress, cell death, and strong growth phenotypes.

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“…LEA protein levels in maturing Arabidopsis and M. truncatula seeds are positively correlated with an increase in seed longevity (Hundertmark et al, 2011;Chatelain et al, 2012). In Arabidopsis seeds and X. viscosa leaves, LEA proteins are broadly distributed in subcellular compartments, reflecting their protective role in the various cellular membranes (Candat et al, 2014;Costa et al, 2017). LEA genes are dehydration inducible and are constitutively expressed in resurrection species (Rodriguez et al, 2010;Jovanovic et al, 2011;Gechev et al, 2013;Giarola et al, 2015a;Costa et al, 2017).…”

Section: Mechanisms For Longevity In the Dry Statementioning

confidence: 99%

Orthodox Seeds and Resurrection Plants: Two of a Kind?

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Cooper

Hilhorst³

et al. 2017

Plant Physiol.

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The Ubiquitous Distribution of Late Embryogenesis Abundant Proteins across Cell Compartments in Arabidopsis Offers Tailored Protection against Abiotic Stress

Cited by 186 publications

References 128 publications

Arabidopsis Seed Mitochondria Are Bioenergetically Active Immediately upon Imbibition and Specialize via Biogenesis in Preparation for Autotrophic Growth

Arabidopsis Seed Mitochondria Are Bioenergetically Active Immediately upon Imbibition and Specialize via Biogenesis in Preparation for Autotrophic Growth

FRIENDLY Regulates Mitochondrial Distribution, Fusion, and Quality Control in Arabidopsis

Orthodox Seeds and Resurrection Plants: Two of a Kind?

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