The Types and Proportions of Commensal Microbiota Have a Predictive Value in Coronary Heart Disease

Chen, Lin; Ishigami, Tomoaki; Doi, Hiroshi; Arakawa, Kentaro; Tamura, Kouichi

doi:10.3390/jcm10143120

Cited by 11 publications

(10 citation statements)

References 42 publications

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“…Our study was the first to use a large amount of clinical sample data. Such a large sample database can improve the accuracy of data analysis and model accuracy, and Chen et al [ 34 ] and Brown and Hazen [ 35 ] suggested that differences in gut microbiota can be used to predict cardiovascular disease. The ultimate goal is to optimize and develop the whole process from sampling device to extraction to library building and sequencing analysis and to achieve rapid, low-cost detection of intestinal flora in a large population [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Analysis of Gut Microbiota in Patients with Coronary Artery Disease and Hypertension

Wan

Zhu

Jin³

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Cardiovascular and cerebrovascular diseases are characterized by high rates of morbidity and mortality. Microbiota is closely associated with cardiovascular disease. We aimed to comprehensively analyze the microbiotas of 300 healthy controls, 300 patients with high blood pressure (HBP), and 300 patients with coronary heart disease (CHD). The results indicated no significant difference in microbiota diversity among the three groups ( P > 0.05 ). However, differences in microbiota richness among the three groups were significant ( P < 0.05 ). Bacteroidetes and Bacteroidia were the dominant bacteria in the CHD group, Enterobacteriales and Escherichia-shigella in the HBP group, and Acidaminococcaceae and Phascolarctobacterium in the healthy control group. The prediction results of the random forest model indicated that the population with CHD displayed prominent features with high sensitivity, indicating that microbiota detection might become a novel clinical indicator to predict and monitor the risk of cardiovascular events. The prediction of microbiota function suggested differences in oxygen supply and chronic inflammation between populations with HBP/CHD and healthy populations. Although there is no difference in gut microbiota diversity among the three groups, each group has its dominant microbiota in terms of richness.

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Section: Resultsmentioning

confidence: 99%

Analysis of Gut Microbiota in Patients with Coronary Artery Disease and Hypertension

Wan

Zhu

Jin³

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…The results revealed that Bacteroides, Lactobacillus , and Rikenellaceae_RC9_gut_group were the groups with significant differential changes in the QS treatment of ACS. Chen 31 reported that Bacteroides were found in data from all 16 cohort studies (2210 patients) in the systematic evaluation assessing the association between commensal microflora and coronary artery disease. A clinical study published in Nature in 2020 found that 32 a high abundance of Bacteroides and a low abundance of Faecalibacterium were associated with inflammation and obesity, and that the administration of atorvastatin was negatively associated with dysbiosis of this flora.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and Mechanism of Qianshan Huoxue Gao in Acute Coronary Syndrome via Regulation of Intestinal Flora and Metabolites

Zhao¹,

Ma²,

Liang³

et al. 2023

DDDT

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Purpose To study the efficacy of Qianshan Huoxue Gao (QS) in treating acute coronary syndrome (ACS) and to explore the mechanism of action from the perspective of intestinal flora regulation. Methods Male Sprague–Dawley rats were divided into control, model, QS, and atorvastatin groups; except for the control group, rats underwent ligation of the left anterior descending branch of the coronary artery. Following treatment for 28 days, cardiac function was evaluated using an echocardiographic assay; ELISAs for serum creatine kinase isoenzyme (CK-MB), cardiac troponin I (cTnI), high-sensitivity C-reactive protein (hs-CRP), interleukin (IL)-2 (IL-2), IL-6, and tumor necrosis factor-α (TNF-α); assessment of cardiac enzymes and inflammatory response; hematoxylin and eosin (HE) staining for histopathological changes in the heart, skin, and viscera; 16S rRNA gene sequencing for intestinal flora diversity and structural differences analysis; and we further investigated intestinal contents using metabolomics. Results Compared with controls, CK-MB and cTnI were increased ( P <0.01); ejection factor and fractional shortening were decreased ( P <0.01); left ventricular internal end-diastolic dimension and left ventricular internal end-systolic dimension were increased ( P <0.01); and IL-2, IL-6, TNF-α, and hs-CRP were increased in the model group. Myocardial damage and inflammation were also observed by HE staining. QS improved these indexes, similar to the atorvastatin group; therefore, QS could effectively treat ACS. QS modulates the structure and abundance of the intestinal flora in ACS model rats, among which Bacteroides, Lactobacillus , and Rikenellaceae_RC9_gut_group are associated with cardiovascular disease. Metabolomics revealed that the intestinal metabolite content changed in ACS, with ethanolamine (EA) being the most relevant metabolite for ACS treatment by QS. EA was significantly positively correlated with Eubacterium xylanophilum group, Ruminococcus, unclassified f__Oscillospiraceae, Intestinimonas, Eubacterium siraeum group, Lachnospiraceae NK4A136 group , and norank f__Desulfovibrionaceae . Conclusion QS can effectively treat ACS and can restore regulation of the intestinal flora. EA may be the primary metabolite of QS, exerting a therapeutic effect in ACS.

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“…Intestinal flora is associated with several scoring points of CHA 2 DS 2 -VAS C . Chen et al [36] studied the specific types of symbiotic flora associated with coronary heart disease (CHD) by systematically reviewing prospective observational studies to evaluate the relationship between symbiotic flora and CHD. Of the 544 published articles identified in the preliminary search, 16 articles from 16 cohort studies (2210 patients) were included in the analysis.…”

Section: Cha 2 Ds 2 -Vas C Score and Intestinal Floramentioning

confidence: 99%

Changes of Intestinal Flora in Patients with Atrial Fibrillation and Its Correlation with Cardiovascular Risk Factors

Chen¹,

Tu²,

Shi³

et al. 2023

Rev. Cardiovasc. Med.

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Background: Based on the 16S rDNA sequence, intestinal flora changes in atrial fibrillation (AF) patients were monitored, the correlation between the changes and CHA2DS2-VASC score was analyzed, and the possible related factors affecting the changes of intestinal flora were investigated. Methods: According to the inclusion criteria, 53 AF patients were selected as atrial fibrillation group (Group AF), detection of C-reactive protein (CRP), homocysteine (Hcy), total bile acid (TBA), brain natriuretic peptide (BNP), High-sensitivity cardiac troponin (Hs-cTn) and left ventricular ejection fraction (LVEF) were accomplished. A total of 29 healthy subjects who underwent physical examination with matched gender and age were selected as the healthy group (Group H), and the same examinations as in Group AF were handled. Structural composition of intestinal flora was detected and analyzed by 16S rRNA sequencing technology. Flora differences between Group AF and Group H were counted, and the correlation analysis among age, Hs-cTn, CRP, TBA, Hcy, BNP and LVEF were explored. Meanwhile, CHA2DS2-VASC score of 53 AF patients was fulfilled, then patients were divided into three subgroups according to different scores, namely: 0 point (AF-0, n = 9), 1 point (AF-1, n = 15), ≥2 points (AF-2, n = 29). Finally, the correlation of intestinal flora differences and CHA2DS2-VASC scores were analyzed. Results: In terms of Alpha diversity, compared with the control group, the abundance and diversity of flora in Group AF were observably reduced. However, at phylum and class level, there was no notable difference in community structure between Group AF and Group H (p > 0.05). Further statistics revealed that the composition and abundance of intestinal flora in Group AF were prominently different from those in Group H at phylum, class, order and family levels, which were correlated with CRP and LVEF. Additionally, bioinformatics analysis comparison was performed on three CHA2DS2-VASC score subgroups of Group AF with Group H. It was reported that at phylum level, the relative abundance of Firmicutes in Group AF-2 and Chloroflexi in Group H was higher. At class level, the relative abundance of Sphingobacteriia, Flavobacteriia and Alphaproteobacteria was higher in group H. At order level, the relative abundance of Sphingobacteriales, Micrococcales, Flavobacteriales, Sphingobacteriales and Rhizobiales in group H was higher. At family level, the relative abundance of Sphingobacteriaceae, Flavobacteriaceae and Clostridiaceae in group H was higher. At genus level, the relative abundance of Sphingobacterium in group H, Clostridiumsensustricto-1 in Group AF-2, Dialister and Allisonella in Group AF-1, and Prevotella-9 in Group AF-0 were higher. Conclusions: There were changes in the relative abundance of intestinal flora at phylum, class, order and family levels, which was concerned with LVEF and CRP value, whereas Alpha diversity index of the flora decreased. The composition and relative abundance of intestinal flora varied in AF patients with CHA2DS2-...

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The Types and Proportions of Commensal Microbiota Have a Predictive Value in Coronary Heart Disease

Cited by 11 publications

References 42 publications

Analysis of Gut Microbiota in Patients with Coronary Artery Disease and Hypertension

Analysis of Gut Microbiota in Patients with Coronary Artery Disease and Hypertension

Efficacy and Mechanism of Qianshan Huoxue Gao in Acute Coronary Syndrome via Regulation of Intestinal Flora and Metabolites

Changes of Intestinal Flora in Patients with Atrial Fibrillation and Its Correlation with Cardiovascular Risk Factors

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