The type of culture medium and the duration of in vitro culture do not influence birthweight of ART singletons

Abstract

None.

Comparative neonatal outcomes in singleton births from blastocyst transfers or cleavage-stage embryo transfers: a systematic review and meta-analysis

“…December 2008 to December 2012Single center1543 single embryo transfers in McGill University Health Center, Canada (day 2/3 vs day 5)In their computerized databaseThe small number of live births resulting from cleavage and blastocyst embryo transfers.No increased risk of maternal or neonatal complications in pregnancies resulting from blastocyst embryo transfers8Maxwell, et al (2015) [25]2003 to 2012Single center392 singleton live births via IVF/ICSI at New York University Fertility Center (day 3 vs day 5/6)In their computerized databaseWomen were young in the blastocyst group.No increased risk of PTB and VPTB8Zhu, et al (2014) [21]January 2009 to June 2012Single center2929 singletons born at Peking University Third Hospital (day 3 vs day 5/6)In their computerized databaseThe number of day 3 transfers was high relative to the number of day 5/6 transfers. No ICSI cycles in the studyNo increased risk of SGA8Ishihara, et al (2014) [23]2008 to 2010National registry277042 singletons born in JapanJapanese ART registry database collected by the Japan Society of Obstetrics and GynecologyWomen were young in the blastocyst transfer group.Blastocyst transfers were associated with a significantly decreased rate of SGA8De Vos, et al (2015) [27]April 2004 to December 2009Single center2098 singleton live births in single center, Belgium (day 3 vs day 5)In their computerized databaseWomen were young in the blastocyst group.The mean singleton birthweights were not different between day 3 embryo transfers and day 5 blastocyst transfers8Makinen, et al (2012)2000 to 2010Single center1079 infants born after treatment at the Family Federation of Finland Fertility Clinic in Helsinki, Finland (day 2/3 vs day 5/6)In their computerized databaseDetails about patients were not clearly described.No increased risk of SGA7 …”

“…Additional studies compared days 2–3 with days 5–6 [6, 13, 26], and one study failed to mention the exact days that were compared altogether [23]. The last three studies compared day 2 with days 5–6 [19], days 2–3 with day 5 [15], and day 3 with day 5 [27]. An additional confounder in some studies arose as a result of including frozen embryo transfers and fresh embryo transfers in the same comparison group [6, 13, 22, 23].…”

Risk of adverse pregnancy and perinatal outcomes after high technology infertility treatment: a comprehensive systematic review

“…Well-designed studies [73–75] have recently evaluated the effect of different types of in vitro culture medium and of the duration of in vitro culture on obstetric and perinatal outcomes in IVF/ICSI populations showing conflicting results, suggesting that data cannot be generalized for all commercial culture media. The different culture medium (Medicult vs. Vitrolife) and the duration of culture (day 3 vs. day 5) had no effect on singleton newborns birthweight, as well as on the incidence of PTB and VPTB, was detected on a large population of 2,098 infertile women treated with fresh IVF and ICSI cycles after controlling for confounders [75].…”

“…The different culture medium (Medicult vs. Vitrolife) and the duration of culture (day 3 vs. day 5) had no effect on singleton newborns birthweight, as well as on the incidence of PTB and VPTB, was detected on a large population of 2,098 infertile women treated with fresh IVF and ICSI cycles after controlling for confounders [75]. Unfortunately, all these studies had a retrospective design and the risk of residual confounding cannot be excluded [72].…”

Effect of mesenchymal stem cells and mouse embryonic fibroblasts on the development of preimplantation mouse embryos

“…According to previous reports the concentration and production of autocrine and/or paracrine factors enhance mouse embryo development and thereby grouped embryos develop better than individually cultivated embryos (Contramaestre et al 2008). Thus, based on the knowledge that ART protocols commonly cultivate the embryos individually (De Vos et al 2015), we hypothesize that coculture with MSC or MEF could improve the development of these embryos and due to the previously described MSC proprieties they could be a better type for ART than MEF. Further studies are required to critically evaluate the conditioned medium in the cocultures and thereby potentially identify embryotrophic factors that can supply adjunctive supplements and improve embryogenesis.…”