The type I collagen induction of MT1-MMP-mediated MMP-2 activation is repressed by αVβ3 integrin in human breast cancer cells

Borrirukwanit, Kulrut; Lafleur, Michel; Mercuri, Francesco; Blick, Tony; Price, John T.; Fridman, Rafael; Pereira, Joseph J.; Leardkamonkarn, Vijittra; Thompson, Erik W.

doi:10.1016/j.matbio.2006.10.014

Cited by 26 publications

(24 citation statements)

References 47 publications

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“…4E,F). In addition, we confirm previous reports (Borrirukwanit et al, 2007;Taherian et al, 2011) showing that MDA-MB-231 cells express moderate levels of aVb3 integrin (Fig. 4E).…”

Section: Adam12-induced Gelatin Degradation Is Caused By Mmp-14supporting

confidence: 92%

“…Moreover, it is well established that MMP-14 associates with aVb3 integrin at the cell surface, and that the cell surface MMP-14 activity can be regulated by aVb3 integrin (Borrirukwanit et al, 2007;Deryugina et al, 2004;Gálvez et al, 2002). On the basis of these data we undertook experiments with parental HEK293 cells, which unlike 293-VnR cells do not exhibit aVb3 integrin expression, but express MMP-14 to the same extent as 293-VnR cells (supplementary material Fig.…”

Section: Adam12-induced Gelatin Degradation Is Caused By Mmp-14mentioning

confidence: 99%

“…In particular, the interaction between MMP-14 and aVb3 integrin has been implicated in a variety of processes involved in tumor progression (Borrirukwanit et al, 2007;Deryugina et al, 2002;Deryugina et al, 2004). Like MMP-14, ADAM12 has been shown to interact with several integrins (i.e.…”

Section: Adam12 Activates Mmp-14 4715mentioning

confidence: 99%

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ADAM12 redistributes and activates MMP-14, resulting in gelatin degradation, reduced apoptosis, and increased tumor growth

Albrechtsen

Kveiborg

Stautz

et al. 2013

Journal of Cell Science

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SummaryMatrix metalloproteinases (MMPs), in particular MMP-2, MMP-9 and MMP-14, play a key role in various aspects of cancer pathology. Likewise, ADAMs (a disintegrin and metalloproteinases), including ADAM12, are upregulated in malignant tumors and contribute to the pathology of cancers. Here, we show that there is a positive correlation between MMP-14 and ADAM12 expression in human breast cancer. We demonstrated that in 293-VnR and human breast cancer cells expressing ADAM12 at the cell surface, endogenous MMP-14 was recruited to the cell surface, resulting in its activation. Subsequent to this activation, gelatin degradation was stimulated and tumor cell apoptosis was decreased, with reduced expression of the pro-apoptotic proteins BCL2L11 and BIK. The effect on gelatin degradation was abrogated by inhibition of the MMP-14 activity and appeared to be dependent on cell surface aVb3 integrin localization, but neither the catalytic activity of ADAM12 nor the cytoplasmic tail of ADAM12 were required. The significance of ADAM12-induced activation of MMP-14 was underscored by a reduction in MMP-14-mediated gelatin degradation and abolition of apoptosis-protective effects by specific monoclonal antibodies against ADAM12. Furthermore, orthotopic implantation of ADAM12-expressing MCF7 cells in nude mice produced tumors with increased levels of activated MMP-14 and confirmed that ADAM12 protects tumor cells against apoptosis, leading to increased tumor progression. In conclusion, our data suggest that a ternary protein complex composed of ADAM12, aVb3 integrin and MMP-14 at the tumor cell surface regulates the function of MMP-14. This interaction might point to a novel concept for the development of MMP-14-targeting drugs in treating cancer.

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“…4E,F). In addition, we confirm previous reports (Borrirukwanit et al, 2007;Taherian et al, 2011) showing that MDA-MB-231 cells express moderate levels of aVb3 integrin (Fig. 4E).…”

Section: Adam12-induced Gelatin Degradation Is Caused By Mmp-14supporting

confidence: 92%

Section: Adam12-induced Gelatin Degradation Is Caused By Mmp-14mentioning

confidence: 99%

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ADAM12 redistributes and activates MMP-14, resulting in gelatin degradation, reduced apoptosis, and increased tumor growth

Albrechtsen

Kveiborg

Stautz

et al. 2013

Journal of Cell Science

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show abstract

“…Integrin avβ3 is expressed on many kinds of cancer cells, such as lung cancer, 20 ovarian cancer, 21 breast cancer 22 and melanoma.…”

Section: Introductionmentioning

confidence: 99%

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

Zhang

et al. 2012

Cancer Biology & Therapy

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These authors contributed equally to this work. Keywords: nanoparticles, albumin, RGD, FITC, delivery, pancreatic cancer, therapyIntegrin avb3 receptor is expressed on several types of cancer cells, including pancreatic cancer cells, and plays an important role in tumor growth and metastasis. The ability to target the integrin avb3 receptor on cancer cells increases the efficacy of targeted therapy and reduces the side effects. The aim of this study is to develop a novel arginine-glycineaspartic acid (RGD) peptide-conjugated albumin nanoparticle to enhance the intracellular uptake of anticancer drug into the pancreatic cancer cells through receptor-mediated endocytosis. In cellular uptake studies, the fluorescent signal of RGD-conjugated BSANPs in BxPC3 cells was higher than that of BSANPs without RGD conjugation as determined by fluorescence spectrophotometer. We also found that BSANPs bound to BxPC3 cells in a time-and concentration-dependent manner. The uptake of RGD-conjugated BSANPs by pancreatic cancer cells was inhibited by an excess amount of free RGD peptide, indicating that the binding and/or uptake were mediated by the avb3 receptor. Furthermore, the nanoparticles were found to be located close to the nuclei by using laser scanning confocal microscopy. Besides, no significant in vitro cytotoxicity was observed as measured with MTT assay. Both in vitro and in vivo antitumor efficacy was improved by targeting gemcitabine-loaded nanoparticles to BxPC-3 cells using RGD peptides. Therefore, the RGD-conjugated BSANPs hold great potential as an effective drug delivery system to deliver therapeutic agents to pancreatic cancer.

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“…Outro trabalho mostrou que anticorpos contra ITGAV (CNTO95) inibem o crescimento celular, migração e invasão através da interrupção de vias mediadas pelas adesões focais (FAK) e os sinais de motilidade celular (Chen et al 2008). Borrirukwanit e colaboradores demonstraram que integrina alfaV beta3 inibe metaloproteinases (MT1-MMP e MMP2) em células humanas de câncer de mama (Borrirukwanit et al 2007). Lu e colaboradores sugerem que o gene ITGAV pode tornar-se um marcador de prognóstico e alvo promissor novo tratamento para carcinoma escamoso de laringe (Lu et al 2009).…”

Section: Ppil1unclassified

Avaliação de marcadores de prognóstico no câncer de mama e análise funcional de CIP4

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The type I collagen induction of MT1-MMP-mediated MMP-2 activation is repressed by αVβ3 integrin in human breast cancer cells

Cited by 26 publications

References 47 publications

ADAM12 redistributes and activates MMP-14, resulting in gelatin degradation, reduced apoptosis, and increased tumor growth

ADAM12 redistributes and activates MMP-14, resulting in gelatin degradation, reduced apoptosis, and increased tumor growth

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

Avaliação de marcadores de prognóstico no câncer de mama e análise funcional de CIP4

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