The type and extent of injuries in vitrified mouse oocytes

Yang, Liang; Ning, Fangyong; Du, Weiting; Wang, Chunsheng; Piao, Shan-Hua; Tie-zhu, AN

doi:10.1016/j.cryobiol.2011.12.003

Cited by 11 publications

(7 citation statements)

References 34 publications

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“…Sufficient time in equilibration solution would allow the blastocoelic contents to be replaced by the permeating cryoprotectants, thereby preventing ice crystal formation [ 29 ]. However, extended exposure to cryoprotectants can exert toxic effects on the embryo, thus a balance between the beneficial and detrimental effects of the cryoprotectants must be considered [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of <i>in vitro</i> produced porcine embryos

Bartolac

Lowe

Koustas

et al. 2015

Journal of Reproduction and Development

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The aim of this study was to determine the optimum conditions for vitrifying in vitro produced day 7 porcine embryos using different vitrification devices and blastocoele collapse methods. Firstly embryos were collapsed by micro-pipetting, needle puncture and sucrose with and without conducting vitrification. In the next experiment, non-collapsed embryos were vitrified in an open device using either superfine open-pulled straws (SOPS) or the CryoLoopTM system, or vitrified in a closed device using either the CryoTipTM or Cryo BioTM’s high security vitrification system (HSV). The post-thaw survival of embryos vitrified in the open devices did not differ significantly (SOPS: 37.3%; CryoLoopTM: 37.3%) nor did the post-thaw survival of embryos vitrified in the closed devices (CryoTip™: 38.5%; HSV: 42.5%). The re-expansion rate of embryos that were collapsed via micro-pipetting (76.0%) did not differ from those that were punctured (75.0%) or collapsed via sucrose (79.6%) when vitrification was not performed. However, embryos collapsed via sucrose solutions (24.5%) and needle puncture (16.0%) prior to vitrification were significantly less likely to survive vitrification than the control (non-collapsed) embryos (53.6%, P < 0.05). The findings show that both open and closed vitrification devices were equally effective for the vitrification of porcine blastocysts. Collapsing blastocysts prior to vitrification did not improve survival, which is inconsistent with the findings of studies in other species. This may be due to the extremely sensitive nature of porcine embryos, and/or the invasiveness of the collapsing procedures.

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Section: Discussionmentioning

confidence: 99%

A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of <i>in vitro</i> produced porcine embryos

Bartolac

Lowe

Koustas

et al. 2015

Journal of Reproduction and Development

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“…It is well known that cryoprotectant exposure is toxic and detrimental to embryo development (Liang et al, ). And in the present study, reduced birth weight was found both in the IVC and VET groups.…”

Section: Discussionmentioning

confidence: 99%

Changes in DNA methylation and imprinting disorders in E9.5 mouse fetuses and placentas derived from vitrified eight‐cell embryos

Wen

et al. 2019

Molecular Reproduction Devel

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Vitrification is increasingly used in assisted reproductive technology (ART) laboratories worldwide, and potential vitrification‐induced risks require further exploration. The effect of vitrification on changes in DNA methylation and imprinting disorders was investigated in E9.5 mouse fetuses and placentas. Fetus and placental tissues were collected from the natural mating (nautural conception [NC]) group, in vitro culture (IVC) group and vitrified embryo transfer (VET) group. The fetal crown‐rump length at E9.5 in both the IVC (0.210 ± 0.059 mm) and VET (0.205 ± 0.048 mm) groups was significantly reduced compared with the NC group (0.288 ± 0.083 mm). The global methylation levels of fetuses were decreased in the IVC group compared with the NC group and it was increased after vitrification compared with IVC (p < 0.05), similar to what was observed in the NC group (p > 0.05). The changes could be attributed to the disorders of DNA methyltransferases and ten‐eleven translocations. In the IVC and VET fetuses, a majority of maternally expressed genes were upregulated, which repressed fetal growth. Furthermore, vitrification led to a change in the methylation level of KvDMR1, which resulted in the disturbance of gene imprinting. According to our results, vitrification could contribute to increased methylation compared with IVC and contributes to a gene imprinting disorder rather than recovery. Despite the routine use of embryo vitrification in clinical settings, the effect that this procedure may have on genomic imprinting deserves much greater attention.

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“…The length of time and the temperature at which oocytes are exposed to cryoprotectants were previously shown to affect post‐warm survival success (Liang et al., ). Oocytes and embryos need to be exposed for sufficient time to allow penetration of the cryoprotectants to occur, but this exposure time should be kept to a minimum to reduce the toxic effects of the cryoprotectants.…”

Section: Discussionmentioning

confidence: 99%

Effect of different penetrating and non‐penetrating cryoprotectants and media temperature on the cryosurvival of vitrified in vitro produced porcine blastocysts

Bartolac

Lowe

Koustas

et al. 2018

Animal Science Journal

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The aim of this study was to determine the most efficient vitrification protocol for the cryopreservation of day 7 in vitro produced (IVP) porcine blastocysts. The post-warm survival rate of blastocysts vitrified in control (17% dimethyl sulfoxide + 17% ethylene glycol [EG] + 0.4 mol/L sucrose) and commercial media did not differ, nor did the post-warm survival rate of blastocysts vitrified in medium containing 1,2-propandiol in place of EG. However, vitrifying embryos in EG alone decreased the cryosurvival rate (55.6% and 33.6%, respectively, p < .05). Furthermore, the post-warm survival rates of blastocysts vitrified with either trehalose or sucrose as the non-penetrating cryoprotectant did not differ. There was also no significant difference in post-warm survival of blastocysts vitrified in control (38°C) media and room temperature (22°C) media with extended equilibration times, although when blastocysts were vitrified using control media at room temperature, the post-warm survival rate increased (56.8%, 57.3%, 72.5%, respectively, p < .05). The findings show that most cryoprotectant combinations examined proved equally effective at supporting the post-warm survival of IVP porcine blastocysts. The improved post-warm survival rate of blastocysts vitrified using media held at room temperature suggests that the cryoprotectant toxicity exerted in 22°C media was reduced.

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The type and extent of injuries in vitrified mouse oocytes

Cited by 11 publications

References 34 publications

A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of <i>in vitro</i> produced porcine embryos

A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of <i>in vitro</i> produced porcine embryos

Changes in DNA methylation and imprinting disorders in E9.5 mouse fetuses and placentas derived from vitrified eight‐cell embryos

Effect of different penetrating and non‐penetrating cryoprotectants and media temperature on the cryosurvival of vitrified in vitro produced porcine blastocysts

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