The type 4 fimbrial subunit gene of Pasteurella multocida

Doughty, Stephen W.; Ruffolo, Carmel G; Adler, Ben

doi:10.1016/s0378-1135(99)00189-3

Cited by 53 publications

(26 citation statements)

References 31 publications

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“…The fact that P. multocida is a commonly encapsulated organism is supported by this study, too. On the other hand, the fimbriae which can play important role in colonisation of host tissue (Doughty et al, 2000;Al Haddawi et al, 2000) have not been described so frequently in P. multocida (Grund et al, 1990), and, in this study, they were found in only four isolates. However, a potential for their production, which did not have to be expressed under the conditions used in this study, can not be excluded among the remaining isolates.…”

Section: Discussioncontrasting

confidence: 69%

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Jaglič¹,

Kučerová²,

Nedbalcová³

et al. 2006

Vet. Med. - Czech

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Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electrophoresis (PFGE) generated by restriction endonuclease ApaI, and examined for the presence of capsule and fimbriae, and tested of haemolytic activity and endotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene, conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin.

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Section: Discussioncontrasting

confidence: 69%

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Jaglič¹,

Kučerová²,

Nedbalcová³

et al. 2006

Vet. Med. - Czech

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“…As we characterized the contig set from our genomic sequencing efforts, we identified a four-gene cluster in the strain 86-028NP genome that was highly homologous to gene loci identified in H. influenzae strain Rd, Actinobacillus pleuropneumoniae, and Pasteurella multocida (14,38,40,52). A. pleuropneumoniae and P. multocida are known to express Tfp, whereas Tfp have not been observed in H. influenzae.…”

Section: Resultsmentioning

confidence: 99%

“…In P. aeruginosa, the biogenesis and function of Tfp are controlled by over 40 genes (42). To date, only a subset of the vast number of related Tfp genes (12,43) has been found in several members of the HAP (Haemophilus, Actinobacillus, and Pasteurella) family (7,13,14,40), and the expression of Tfp, or a twitching phenotype, has not been described for any H. influenzae isolate. In fact, H. influenzae is classically described as a bacterium that does not express these structures (1,8,18,19), despite the presence of a cryptic gene cluster within the strain Rd genome (16).…”

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confidence: 99%

Demonstration of Type IV Pilus Expression and a Twitching Phenotype by Haemophilus influenzae

et al. 2005

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Haemophilus influenzae is considered a nonmotile organism that expresses neither flagella nor type IV pili, although H. influenzae strain Rd possesses a cryptic pilus locus. We demonstrate here that the homologous gene cluster pilABCD in an otitis media isolate of nontypeable H. influenzae strain 86-028NP encodes a surface appendage that is highly similar, structurally and functionally, to the well-characterized subgroup of bacterial pili known as type IV pili. This gene cluster includes a gene (pilA) that likely encodes the major subunit of the heretofore uncharacterized H. influenzae-expressed type IV pilus, a gene with homology to a type IV prepilin peptidase (pilD) as well as two additional uncharacterized genes (pilB and pilC). A second gene cluster (comABCDEF) was also identified by homology to other pil or type II secretion system genes. When grown in chemically defined medium at an alkaline pH, strain 86-028NP produces approximately 7-nm-diameter structures that are near polar in location. Importantly, these organisms exhibit twitching motility. A mutation in the pilA gene abolishes both expression of the pilus structure and the twitching phenotype, whereas a mutant lacking ComE, a Pseudomonas PilQ homologue, produced large appendages that appeared to be membrane bound and terminated in a slightly bulbous tip. These latter structures often showed a regular pattern of areas of constriction and expansion. The recognition that H. influenzae possesses a mechanism for twitching motility will likely profoundly influence our understanding of H. influenzae-induced diseases of the respiratory tract and their sequelae.

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“…The Type IV fimbriae are formed by repeated subunits and present a highly conserved N-terminal nucleotide sequence (Hatfaludi et al 2010). In a recent study, Sellyei et al (2010) identified only two major alleles of ptfA among 31 strains of different sero- Doughty et al (2000), this gene is a candidate for heterologous vaccine development (Ewers et al 2006). The hemagglutinin PfhA was identified in 15/25 strains (60%).…”

Section: Discussionmentioning

confidence: 99%

Detection of virulence-associated genes of Pasteurella multocida isolated from cases of fowl cholera by multiplex-PCR

et al. 2013

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The current systems of breeding poultry, based on high population density, increase the risk of spreading pathogens, especially those causing respiratory diseases and those that have more than one host. Fowl Cholera (FC) is one such pathogen, and even though it represents one of several avian diseases that should be considered in the differential diagnosis of notifiable diseases that present with sudden death, the pathogenesis and virulence factors involved in FC are still poorly understood. The objective of this study was to investigate twelve genes related to virulence in 25 samples of Pasteurella multocida isolated from FC cases in the southern region of Brazil through the development of multiplex PCR protocols. The protocols developed were capable of detecting all of the proposed genes. The ompH, oma87, sodC, hgbA, hgbB, exBD-tonB and nanB genes were present in 100% of the samples (25/25), the sodA and nanH genes were present in 96% (24/25), ptfA was present in 92% (23/25), and pfhA was present in 60% (15/25). Gene toxA was not identified in any of the samples studied (0/25). Five different genetic profiles were obtained, of which P1 (negative to toxA) was the most common. We concluded that the multiplex-PCR protocols could be useful tools for rapid and simultaneous detection of virulence genes. Despite the high frequency of the analyzed genes and the fact that all samples belonged to the same subspecies of P. multocida, five genetic profiles were observed, which should be confirmed in a study with a larger number of samples.

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The type 4 fimbrial subunit gene of Pasteurella multocida

Cited by 53 publications

References 31 publications

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Demonstration of Type IV Pilus Expression and a Twitching Phenotype by Haemophilus influenzae

Detection of virulence-associated genes of Pasteurella multocida isolated from cases of fowl cholera by multiplex-PCR

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