The two-electron reduced A cluster in acetyl-CoA synthase: Preparation, characteristics and mechanistic implications

Gencic, Simonida; Duin, Evert C.; Grahame, David A.

doi:10.1016/j.jinorgbio.2022.112098

Cited by 4 publications

(3 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A ring critical point is found between one of the Fe-S edges of the cluster and the Cys488-Nip bond, forming a 4-membered ring resembling one of the faces of the cubane. Previous experimental and theoretical studies proposed significant rearrangements between tetrahedral and square planar geometries during catalysis (42). Based on our calculations, it is feasible that a low-valent Nip is coordinated by a sulfide of the [4Fe-4S] cluster, generating the extended cubane structure observed in the experiment.…”

Section: Methyl Transfer Reaction Through B12 Domain Motionsupporting

confidence: 61%

Snapshots of acetyl-CoA synthesis, the final step of CO₂fixation in the Wood-Ljungdahl pathway

Yin,

Lemaire,

Rosas Jiménez

et al. 2024

Preprint

View full text Add to dashboard Cite

In the ancient microbial Wood-Ljungdahl pathway, CO2is fixed in a multi-step process ending with acetyl-CoA synthesis at the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase complex (CODH/ACS). Here, we present catalytic snapshots of the CODH/ACS from the gas-converting acetogenClostridium autoethanogenum, characterizing the molecular choreography of the overall reaction including electron transfer to the CODH for CO2reduction, methyl transfer from the corrinoid iron-sulfur protein (CoFeSP) partner to the ACS active site and acetyl-CoA production. Unlike CODH, the multidomain ACS undergoes large conformational changes to form an internal connection to the CODH active site, accommodate the CoFeSP for methyl transfer and protect the reaction intermediates. Altogether, the structures allow us to draw a detailed reaction mechanism of this enzyme crucial for CO2fixation in anaerobic organisms.One-Sentence Summary:Structural description of key states of CO2fixation by the carbon monoxide dehydrogenase/acetyl-CoA synthase complex.

show abstract

Section: Methyl Transfer Reaction Through B12 Domain Motionsupporting

confidence: 61%

Snapshots of acetyl-CoA synthesis, the final step of CO₂fixation in the Wood-Ljungdahl pathway

Yin,

Lemaire,

Rosas Jiménez

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In addition, five subunits of CODH/ACS from some Methanosarcina species were detected: the α2 subunit (A0A0E3LTN4; 41 P, 3 UP) of M. mazei Goe1, β1 subunit (A0A0E3NLE4; 38 P, 12 UP) of M. thermophila , δ subunit (A0A0E3LEU3; 26 P, 5 UP) of M. mazei WWM610, and ε subunit (A0A0E3LSW0; 19 P, 8 UP) of M. mazei SarPi ( Table S5 ). This complex has been reported in the oligomeric form α 6 β 6 γ 6 δ 6 ε 6 and catalyzes the formation/cleavage of the acetyl C–C bond of acetyl-CoA by an Ni and Fe 4 S 4 catalytic site using a two-electron reduction with a negative potential of −495 mV [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

An Initial Proteomic Analysis of Biogas-Related Metabolism of Euryarchaeota Consortia in Sediments from the Santiago River, México

et al. 2023

View full text Add to dashboard Cite

In this paper, sediments from the Santiago River were characterized to look for an alternative source of inoculum for biogas production. A proteomic analysis of methane-processing archaea present in these sediments was carried out. The Euryarchaeota superkingdom of archaea is responsible for methane production and methane assimilation in the environment. The Santiago River is a major river in México with great pollution and exceeded recovery capacity. Its sediments could contain nutrients and the anaerobic conditions for optimal growth of Euryarchaeota consortia. Batch bioreactor experiments were performed, and a proteomic analysis was conducted with current database information. The maximum biogas production was 266 NmL·L−1·g VS−1, with 33.34% of methane, and for proteomics, 3206 proteins were detected from 303 species of 69 genera. Most of them are metabolically versatile members of the genera Methanosarcina and Methanosarcinales, both with 934 and 260 proteins, respectively. These results showed a diverse euryarcheotic species with high potential to methane production. Although related proteins were found and could be feeding this metabolism through the methanol and acetyl-CoA pathways, the quality obtained from the biogas suggests that this metabolism is not the main one in carbon use, possibly the sum of several conditions including growth conditions and the pollution present in these sediments

show abstract

“…Renewed interest in the ACS active site signifies the necessity for new model compounds in the development of catalysts that can valorize CO 2 into fuels. [34][35][36] Ongoing studies will leverage the knowledge obtained in this study towards bimetallic activation of small molecules.…”

Section: Dalton Transactions Papermentioning

confidence: 99%

Site specific redox properties in ligand differentiated di-nickel complexes inspired by the acetyl CoA synthase active site

Quiroz,

Jana,

Liu

et al. 2024

Dalton Trans.

View full text Add to dashboard Cite

show abstract

The two-electron reduced A cluster in acetyl-CoA synthase: Preparation, characteristics and mechanistic implications

Cited by 4 publications

References 54 publications

Snapshots of acetyl-CoA synthesis, the final step of CO₂fixation in the Wood-Ljungdahl pathway

Snapshots of acetyl-CoA synthesis, the final step of CO₂fixation in the Wood-Ljungdahl pathway

An Initial Proteomic Analysis of Biogas-Related Metabolism of Euryarchaeota Consortia in Sediments from the Santiago River, México

Site specific redox properties in ligand differentiated di-nickel complexes inspired by the acetyl CoA synthase active site

Contact Info

Product

Resources

About

The two-electron reduced A cluster in acetyl-CoA synthase: Preparation, characteristics and mechanistic implications

Cited by 4 publications

References 54 publications

Snapshots of acetyl-CoA synthesis, the final step of CO2fixation in the Wood-Ljungdahl pathway

Snapshots of acetyl-CoA synthesis, the final step of CO2fixation in the Wood-Ljungdahl pathway

An Initial Proteomic Analysis of Biogas-Related Metabolism of Euryarchaeota Consortia in Sediments from the Santiago River, México

Site specific redox properties in ligand differentiated di-nickel complexes inspired by the acetyl CoA synthase active site

Contact Info

Product

Resources

About

Snapshots of acetyl-CoA synthesis, the final step of CO₂fixation in the Wood-Ljungdahl pathway

Snapshots of acetyl-CoA synthesis, the final step of CO₂fixation in the Wood-Ljungdahl pathway