The Two DUF642 At5g11420 and At4g32460-Encoded Proteins Interact In Vitro with the AtPME3 Catalytic Domain

Zúñiga-Sánchez, Esther; Gamboa‐deBuen, Alicia

doi:10.5772/37984

Cited by 8 publications

(10 citation statements)

References 71 publications

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“…However, there are still no functional studies that confirm this interaction. BDX and At5g11420 interact in vitro with a PME [12]. Studies in different plant species suggest that their function could be related to the regulation of HGs modification throughout plant development.…”

Section: Discussionmentioning

confidence: 99%

“…The protein encoded by At3g08030 (Clade A2) interacted specifically with cellulose [11]. The proteins encoded by At4g32460 / BDX and At5g11420 (Clade A1) interacted with the catalytic domain of a pectin methyl esterase (PME) [12]. In two transgenic Arabidopsis lines overexpressing BDX the PME activity increased in the seeds, seedlings, and the apical meristems, but no changes in PME activity were detected in the leaves [13].…”

Section: Introductionmentioning

confidence: 99%

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Overview of the Role of Cell Wall DUF642 Proteins in Plant Development

Cruz-Valderrama

Gómez-Maqueo

Salazar-Iribe

et al. 2019

IJMS

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The DUF642 protein family is found exclusively in spermatophytes and is represented by 10 genes in Arabidopsis and in most of the 24 plant species analyzed to date. Even though the primary structure of DUF642 proteins is highly conserved in different spermatophyte species, studies of their expression patterns in Arabidopsis have shown that the spatial-temporal expression pattern for each gene is specific and consistent with the phenotypes of the mutant plants studied so far. Additionally, the regulation of DUF642 gene expression by hormones and environmental stimuli was specific for each gene, showing both up- and down-regulation depending of the analyzed tissue and the intensity or duration of the stimuli. These expression patterns suggest that the DUF642 genes are involved throughout the development and growth of plants. In general, changes in the expression patterns of DUF642 genes can be related to changes in pectin methyl esterase activity and/or to changes in the degree of methyl-esterified homogalacturonans during plant development in different cell types. Thus, the regulation of pectin methyl esterases mediated by DUF642 genes could contribute to the regulation of the cell wall properties during plant growth.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overview of the Role of Cell Wall DUF642 Proteins in Plant Development

Cruz-Valderrama

Gómez-Maqueo

Salazar-Iribe

et al. 2019

IJMS

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“…Among the strongest modifications are proteins having the domain of unknown function DUF642 (At1g29980, ↗15; At3g08030, ↗2.9; At5g25460, ↘21; At5g11420, ↘20). The DUF642 family proteins are reported to localize in the cell wall polysaccharide fraction [59], where At5g11420 interacts in vitro with the catalytic domain of the pectin methylesterase AtPME3 [60]. Surprisingly, although At3g08030 transcripts have been described as a marker for seed germination performance [61], vps29 seeds, which have higher levels of the protein compared with the wild-type, exhibit a lower vigor.…”

Section: Discussionmentioning

confidence: 99%

Combined Proteomic and Metabolomic Profiling of the Arabidopsis thaliana vps29 Mutant Reveals Pleiotropic Functions of the Retromer in Seed Development

Durand

Cueff

Godin

et al. 2019

IJMS

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The retromer is a multiprotein complex conserved from yeast to humans, which is involved in intracellular protein trafficking and protein recycling. Selection of cargo proteins transported by the retromer depends on the core retromer subunit composed of the three vacuolar protein sorting (VPS) proteins, namely VPS26, VPS29, and VPS35. To gain a better knowledge of the importance of the plant retromer in protein sorting, we carried out a comparative proteomic and metabolomic analysis of Arabidopsis thaliana seeds from the wild-type and the null-retromer mutant vps29. Here, we report that the retromer mutant displays major alterations in the maturation of seed storage proteins and synthesis of lipid reserves, which are accompanied by severely impaired seed vigor and longevity. We also show that the lack of retromer components is counterbalanced by an increase in proteins involved in intracellular trafficking, notably members of the Ras-related proteins in brain (RAB) family proteins. Our study suggests that loss of the retromer stimulates energy metabolism, affects many metabolic pathways, including that of cell wall biogenesis, and triggers an osmotic stress response, underlining the importance of retromer function in seed biology.

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“…In this respect, it should be noted that SPL3 is directly regulated by SOC1 [62]. Furthermore, some members of the DUF642 protein family directly interact with pectin methylesterase (PME) isoforms, generating the hypothesis that they modulate the pectin methylesterified state in vivo and are thus involved in the fine-tuning of the biomechanical properties of the cell wall [18,58]. The involvement of DUF642 proteins in cell elongation in young tissues was recently shown [63], and the identification of these conserved promoter motifs as binding sites for transcription factors involved in cell wall loosening may contribute to the further disentanglement of their physiological function.…”

Section: Discussionmentioning

confidence: 99%

“…Restructuration of the cell wall during development is performed by the regulated activity of cell wall-located proteins that target xylans [12], polygalacturonans [13], glycoproteins [14], galacto-oligosaccharides [15], cellulose [16], or lignin synthesis [17]. Moreover, proteins with a domain of unknown function (DUF) were identified in cell wall proteome studies (among others DUF26, DUF231, DUF246, DUF248, DUF288, DUF642, DUF1005, DUF1680), all of which may have a function in cell wall development [16,18].…”

Section: Introductionmentioning

confidence: 99%

The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems

Sergeant

Printz

Guerriero

et al. 2019

Biology

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In this study, the cell-wall-enriched subproteomes at three different heights of alfalfa stems were compared. Since these three heights correspond to different states in stem development, a view on the dynamics of the cell wall proteome during cell maturation is obtained. This study of cell wall protein-enriched fractions forms the basis for a description of the development process of the cell wall and the linking cell wall localized proteins with the evolution of cell wall composition and structure. The sequential extraction of cell wall proteins with CaCl2, EGTA, and LiCl-complemented buffers was combined with a gel-based proteome approach and multivariate analysis. Although the highest similarities were observed between the apical and intermediate stem regions, the proteome patterns are characteristic for each region. Proteins that bind carbohydrates and have proteolytic activity, as well as enzymes involved in glycan remobilization, accumulate in the basal stem region. Beta-amylase and ferritin likewise accumulate more in the basal stem segment. Therefore, remobilization of nutrients appears to be an important process in the oldest stem segment. The intermediate and apical regions are sites of cell wall polymer remodeling, as suggested by the high abundance of proteins involved in the remodeling of the cell wall, such as xyloglucan endoglucosylase, beta-galactosidase, or the BURP-domain containing polygalacturonase non-catalytic subunit. However, the most striking change between the different stem parts is the strong accumulation of a DUF642-conserved domain containing protein in the apical region of the stem, which suggests a particular role of this protein during the early development of stem tissues.

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The Two DUF642 At5g11420 and At4g32460-Encoded Proteins Interact In Vitro with the AtPME3 Catalytic Domain

Cited by 8 publications

References 71 publications

Overview of the Role of Cell Wall DUF642 Proteins in Plant Development

Overview of the Role of Cell Wall DUF642 Proteins in Plant Development

Combined Proteomic and Metabolomic Profiling of the Arabidopsis thaliana vps29 Mutant Reveals Pleiotropic Functions of the Retromer in Seed Development

The Dynamics of the Cell Wall Proteome of Developing Alfalfa Stems

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