The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR

Abstract

The interaction of human galectin 8 and its two separate N-terminal and C-terminal carbohydrate recognition domains (CRD) to their natural ligands has been analysed using a synergistic combination of experimental...

Selective 13C‐Labels on Repeating Glycan Oligomers to Reveal Protein Binding Epitopes through NMR: Polylactosamine Binding to Galectins

“…Binding of the differently labeled tri-LacNAc compounds (5a, 5b, 5c)t ot he selected galectins was monitored through 2D STD-1 H, 13 C-HSQC NMR experiments.C onventional 1D 1 H-STD NMR experiments have been extensively used to study the recognition of b-galactose containing glycans by galectins, [25,26] whereby the strongest STD effect systematically corresponds to protons H6, H5 and H4 of the b-galactose residue,c entral element in these binding events,p roviding most of the intermolecular interactions,including akey CH-p stacking with ac onserved tryptophan (Trp) residue at the galectin binding site. [27,28] In the regular HSQC spectrum of the tri-labeled compound 5a (Figure 2a 13 C-HSQC spectra.…”

Glycosidic α-linked mannopyranose disaccharides: an NMR spectroscopy and molecular dynamics simulation study employing additive and Drude polarizable force fields

“…Thus, the present study represents an important step forward in obtaining a more detailed understanding of the conformational properties of mannose saccharides that builds upon four decades of combing experimental methods with molecular modeling. Furthermore, future applications to complex structures or assemblies such as in studies of carbohydrateprotein interactions [126][127][128] may be aided by the improved description of the physical forces dictating the conformational properties obtained with the Drude polarizable force field model thereby representing molecular interactions in a more realistic way.…”

Oligosaccharide Presentation Modulates the Molecular Recognition of Glycolipids by Galectins on Membrane Surfaces

“…The protein expression and purification are described elsewhere [50,51]. Briefly, proteins' sequence in pET11a vector was transformed in BL21(DE3) E. coli.…”