The tumor suppressor CDX2 opposes pro-metastatic biomechanical modifications of colon cancer cells through organization of the actin cytoskeleton

Platet, Nadine; Hinkel, Isabelle; Richert, Ludovic; Murdamoothoo, Devadarssen; Moufok-Sadoun, Ahlam; Vanier, Marie T.; Lavalle, Philippe; Gaiddon, Christian; Vautier, Dominique; Freund, Jean–Noël; Groß, Isabelle

doi:10.1016/j.canlet.2016.10.040

Cited by 29 publications

(20 citation statements)

References 52 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings further indicated that CDX2 was poorly expressed in breast cancer, while the over-expressed CDX2 diminished the migration and invasion abilities of breast cancer epithelial cells. As a homeotic transcriptional factor, CDX2 was poorly expressed in colon cancer and associated with invasion and metastasis of cells [17]. Therefore, it would be reasonable to suggest that poor expression of CDX2 is correlated with migration and invasion of breast cancer epithelial cells, and could be countered by over-expression of CDX2.…”

Section: Discussionmentioning

confidence: 99%

Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression

et al. 2020

View full text Add to dashboard Cite

Background: microRNA Let-7 serves as a tumor suppressor by targeting various oncogenic pathways in cancer cells. However, the underlying mechanism of its involvement in breast cancer remains largely unknown. With our research, our endeavor is to explore the role of the CDX2/let-7b/COL11A1 axis in breast cancer cell activities. Methods: Tumor tissues and adjacent normal tissues were collected from 86 patients with breast cancer. Human breast cancer epithelial cell line MCF-7 was treated with over-expressed CDX2, let-7b mimic, shRNA against COL11A1 and their negative controls. The expression of CDX2, let-7b, and COL11A1 in the tissues and cells was determined by RT-qPCR. Interactions among CDX2, let-7b, and COL11A1 were detected by ChIP and dual-luciferase reporter assay, respectively. After different transfections, cell invasion, migration, and proliferation abilities were determined by Transwell and EdU assays. Lastly, tumor xenografts in nude mice were established and hematoxylin and eosin staining was performed to assess the tumor growth and lymph node metastasis. Results: CDX2 and let-7b were poorly expressed in breast cancer cells and tissues. CDX2 bound to let-7b and promoted the expression of let-7b, which contrarily inhibited the expression of COL11A1. Cancer cell proliferation, invasion, migration, and metastasis were stimulated when CDX2 and let-7b were depleted or COL11A1 was overexpressed. Xenograft tumors growth and metastasis were in accordance with the results of cellular experiments. Conclusion: In agreement with these observations, we could reach a conclusion that CDX2 could promote let-7b expression, which may exert an inhibitory effect on the proliferation, migration, and metastasis of breast cancer cells via repressing the expression of COL11A1, providing a novel therapeutic strategy for the treatment of metastatic breast cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This surface is made of patches of Cdx2 -intact cells, Cdx2 -depleted cells, and also eroded areas. Previous studies have shown that reducing the Cdx2 level in mice increases intestinal permeability and sensitivity to proinflammatory treatment ( Calon et al, 2007 ), and that Cdx2 modulates the motility and mechanical properties of the intestinal epithelial cells ( Gross et al, 2008 ; Platet et al, 2017 ). Therefore, the mosaic deletion of Cdx2 creates a complex picture with abrasion and tissue repair from Cdx2 -intact and Cdx2 -deleted cells, which may contribute to the resulting modification of the underpinning stroma.…”

Section: Discussionmentioning

confidence: 99%

The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non–cell-autonomous mechanisms

Balbinot

Armant

Elarouci

et al. 2018

Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…Despite the wide application of therapies such as surgery, radiation, and systemic chemoradiotherapy, it remains a risk to human health due to high recurrence and poor survival . Several molecular biomarkers have been widely studied as a method to devise new treatment strategies and improve the prognosis for CRC patients.…”

Section: Introductionmentioning

confidence: 99%

S100A16 is a prognostic marker for colorectal cancer

Sun

Wang

Zhang

et al. 2017

Journal of Surgical Oncology

View full text Add to dashboard Cite

show abstract

The tumor suppressor CDX2 opposes pro-metastatic biomechanical modifications of colon cancer cells through organization of the actin cytoskeleton

Cited by 29 publications

References 52 publications

Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression

Over-expression of CDX2 alleviates breast cancer by up-regulating microRNA let-7b and inhibiting COL11A1 expression

The Cdx2 homeobox gene suppresses intestinal tumorigenesis through non–cell-autonomous mechanisms

S100A16 is a prognostic marker for colorectal cancer

Contact Info

Product

Resources

About