The Tumor Necrosis Factor α (-308 A/G) Polymorphism Is Associated with Cystic Fibrosis in Mexican Patients

Abstract

Environmental and genetic factors may modify or contribute to the phenotypic differences observed in multigenic and monogenic diseases, such as cystic fibrosis (CF). An analysis of modifier genes can be helpful for estimating patient prognosis and directing preventive care. The aim of this study is to determine the association between seven genetic variants of four modifier genes and CF by comparing their corresponding allelic and genotypic frequencies in CF patients (n = 81) and control subjects (n = 104). Genetic variants of MBL2 exon 1 (A, B, C and D), the IL-8 promoter (−251 A/T), the TNFα promoter (TNF1/TNF2), and SERPINA1 (PI*Z and PI*S) were tested in CF patients and control subjects from northeastern Mexico by PCR-RFLP.ResultsThe TNF2 allele (P = 0.012, OR 3.43, 95% CI 1.25–9.38) was significantly associated with CF under the dominant and additive models but was not associated with CF under the recessive model. This association remained statistically significant after adjusting for multiple tests using the Bonferroni correction (P = 0.0482). The other tested variants and genotypes did not show any association with the disease.ConclusionAn analysis of seven genetic variants of four modifier genes showed that one variant, the TNF2 allele, appears to be significantly associated with CF in Mexican patients.

Steroid 5 alpha-reductase 2 enzyme variants, biomass exposure and tobacco use in Mexican patients with prostate cancer

“…Each blood sample was centrifuged at 3,857 g for 10 min at room temperature. From the buffy coat, DNA extraction was performed using a previously reported method with TSNT lysis buffer (composed of 1% Triton, 1% sodium dodecyl sulfate, 100 mM NaCl, 10 mM Tris-HCl pH 8.0 and 1 mM EDTA) and followed by a phenol-chloroform extraction step ( 27 , 28 ). The DNA was precipitated from aqueous phase with ethanol and quantified using NanoDrop 1000 (Thermo Fisher Scientific, Inc.) and its quality was verified through absorbance ratios (260/280 and 260/230 nm) and agarose gel electrophoresis.…”