The trypsin inhibitors present in seed of different grain legume species and cultivar

Guillamón, Eva; Pedrosa, Mercedes M.; Burbano, Carmen; Cuadrado, Carmen; Sánchez, Marı́a de Cortes; Múzquiz, M.

doi:10.1016/j.foodchem.2007.07.029

Cited by 153 publications

(118 citation statements)

References 35 publications

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“…This property is mainly attributed to a large number of internal disulfide bonds and polar interactions between subdomains 19 . Legume protease inhibitors have no carbohydrates and belong to Kunitz trypsin inhibitors (KTI) or Bowman-Birk inhibitors (BBI) 20 . The molecular weights of BBI vary from 1513 to 20 000 Da and they inhibit both trypsin and chymotrypsin, while KTI are larger and exhibit strong inhibition effect on trypsin 21 .…”

Section: Discussionmentioning

confidence: 99%

Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

Dan

2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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A 16-kDa trypsin inhibitor was isolated from an edible legume using various chromatographic procedures. The protein was unadsorbed on Affi-gel blue gel but adsorbed on DEAE-Sepharose and Mono Q following which media the protein was subsequently subjected to gel filtration on Superdex 75 and a final 21-fold purification was achieved. This trypsin inhibitor showed remarkable pH and thermal stability. Its inhibitory activity was impaired in the presence of 1 mM dithiothreitol. The anti-proliferative and anti-mobility activities of this trypsin inhibitor and a hemagglutinin isolated from the same legume were tested on nasopharyngeal carcinoma cells. These two defense proteins demonstrated discrepant anti-proliferative efficacies that the hemagglutinin could greatly suppress the proliferation of nasopharyngeal carcinoma cells, while the trypsin inhibitor revealed a minor effect. However, these two proteins could both attenuate the mobility of nasopharyngeal carcinoma cells. The present study revealed the potential of applying plant defense proteins in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

Dan

2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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show abstract

“…To other manifestations belong inflammatory diseases of the pancreas after feeding animals with soybean powder leading up to hypertrophy and hyperplasia of the pancreas with the hypersecretion of digestive enzymes. (Guillamona et al 2008) The increased secretion of the pancreatic juice leads to higher secretion of the fecal nitrogen and possible deficiency of some aminoacids, the sulphur aminoacid in particular. (Belitz, Grosh, 1992;Guillamona et al, 2008).…”

Section: Occurrence and Properties And Physiological Functionmentioning

confidence: 99%

“…(Guillamona et al 2008) The increased secretion of the pancreatic juice leads to higher secretion of the fecal nitrogen and possible deficiency of some aminoacids, the sulphur aminoacid in particular. (Belitz, Grosh, 1992;Guillamona et al, 2008). Many proteinase inhibitors show the same active centre as is the part of the peptide enzymes binding, whose function they inhibit.…”

Section: Occurrence and Properties And Physiological Functionmentioning

confidence: 99%

“…In a molecule it contains 7 disulphide bridges and a circular arrangement of reactive sites on inhibition and by that it gains higher thermal stability, stability towards acids as well as proteolysis by pepsin. It was demonstrated that the daily discarded amount of trypsin and chymotrypsin is completely inactivated by the intake of 100 g soya beans (Guillamona et al, 2008). The positive or negative impact of the protein inhibitors is given by their received amount, but also by the kind of soya cultivar.…”

Section: Occurrence and Properties And Physiological Functionmentioning

confidence: 99%

“…This knowledge has led to breeding of soya cultivars with low up to zero activity of the Kunitz and Bowman-Birk inhibitor. (Guillamona et al, 2008). The Kunitz and Bowman-Birk soya inhibitor increase secretion of cholecystokinin (CCK) by intestinal mucosa.…”

Section: Occurrence and Properties And Physiological Functionmentioning

confidence: 99%

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