The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling

Plana-Carmona, Marcos; Stik, Grégoire; Bulteau, Romain; Segura-Morales, Carolina; Alcázar, Noelia; Wyatt, Christopher D R; Klonizakis, Antonios; Andrés-Aguayo, Luísa de; Gasnier, Maxime; Tian, Tian V.; Garcia, Guillem Torcal; Vila-Casadesús, María; Plachta, Nicolas; Serrano, Manuel; Francesconi, Mirko; Graf, Thomas

doi:10.1016/j.stemcr.2022.07.009

Cited by 7 publications

(4 citation statements)

References 45 publications

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“…CEBPA is expressed in several cell lines derived from myeloid, liver, and placental cancers (Figure S4F). Moreover, we recently described the expression of CEBPA in the trophectoderm of blastocysts 53 and therefore immunostained mouse blastocysts for CEBPA (Figure S4G). Trophectoderm cells showed small nuclear CEBPA puncta as well as larger conglomerates with a distribution distinct from chromatin-dense regions and splicing speckles (Figures S4H).…”

Section: Native Cebpa Drives the Formation Of Nuclear Focimentioning

confidence: 99%

CEBPA Phase Separation Links Transcriptional Activity and 3D Chromatin Hubs

et al. 2022

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Section: Native Cebpa Drives the Formation Of Nuclear Focimentioning

confidence: 99%

CEBPA Phase Separation Links Transcriptional Activity and 3D Chromatin Hubs

et al. 2022

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“…The increase in NO generation elicited by LPS (5 μg/mL) was completely abolished in presence of TAK‐242 (1 μM) (Figure 5C and Figure S1), indicating activation of TLR4 signalling under these experimental conditions. Interestingly TAK‐242 (1 μM) partially downregulated the intrinsic NO generation of embryoid bodies, which may suggest that inflammatory signalling cascades may take place during early embryogenesis 18 …”

Section: Resultsmentioning

confidence: 98%

“…Interestingly TAK‐242 (1 μM) partially downregulated the intrinsic NO generation of embryoid bodies, which may suggest that inflammatory signalling cascades may take place during early embryogenesis. 18 …”

Section: Resultsmentioning

confidence: 99%

The pro‐inflammatory signature of lipopolysaccharide in spontaneous contracting embryoid bodies differentiated from mouse embryonic stem cells

Scharmacher,

Wartenberg,

Sauer

2023

J Cellular Molecular Medi

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Embryonic stem (ES) cells differentiate towards all three germ layers, including cardiac cells and leukocytes, and may be therefore suitable to model inflammatory reactions in vitro. In the present study, embryoid bodies differentiated from mouse ES cells were treated with increasing doses of lipopolysaccharide (LPS) to mimic infection with gram‐negative bacteria. LPS treatment dose‐dependent increased contraction frequency of cardiac cell areas and calcium spikes and increased protein expression of α‐actinin. LPS treatment increased the expression of the macrophage marker CD68 and CD69, which is upregulated after activation on T cells, B cells and NK cells. LPS dose‐dependent increased protein expression of toll‐like receptor 4 (TLR4). Moreover, upregulation of NLR family pyrin domain containing 3 (NLRP3), IL‐1ß and cleaved caspase 1 was observed, indicating activation of inflammasome. In parallel, generation of reactive oxygen species (ROS), nitric oxide (NO), and expression of NOX1, NOX2, NOX4 and eNOS occurred. ROS generation, NOX2 expression and NO generation were downregulated by the TLR4 receptor antagonist TAK‐242 which abolished the LPS‐induced positive chronotropic effect of LPS. In conclusion, our data demonstrate that LPS induced a pro‐inflammatory cellular immune response in tissues derived from ES cells, recommending the in vitro model of embryoid bodies for inflammation research.

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“…A microarray analysis of the mid-secretory phase endometrium of patients suffering from recurrent implantation failure show a lower expression of IL-6, compared to healthy controls [ 28 ], further supporting the role of IL-6 in endometrial receptivity. Blastocysts also express and secrete IL-6 [ 29 , 30 , 31 , 32 ], and IL-6 in vitro treatment increases the embryos’ blastulation and hatching rates, compared to untreated embryos [ 33 ]. Furthermore, a higher IL-6 level in the follicular fluid also correlates with implantation success in patients undergoing in vitro fertilization (IVF) [ 34 ].…”

Section: Il-6 and Il-8 In A Healthy Pregnancymentioning

confidence: 99%

IL-6 and IL-8: An Overview of Their Roles in Healthy and Pathological Pregnancies

Vilotić

Nacka-Aleksić

Pirković

et al. 2022

IJMS

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Interleukin-6 (IL-6) is an acknowledged inflammatory cytokine with a pleiotropic action, mediating innate and adaptive immunity and multiple physiological processes, including protective and regenerative ones. IL-8 is a pro-inflammatory CXC chemokine with a primary function in attracting and activating neutrophils, but also implicated in a variety of other cellular processes. These two ILs are abundantly expressed at the feto-maternal interface over the course of a pregnancy and have been shown to participate in numerous pregnancy-related events. In this review, we summarize the literature data regarding their role in healthy and pathological pregnancies. The general information related to IL-6 and IL-8 functions is followed by an overview of their overall expression in cycling endometrium and at the feto-maternal interface. Further, we provide an overview of their involvement in pregnancy establishment and parturition. Finally, the implication of IL-6 and IL-8 in pregnancy-associated pathological conditions, such as pregnancy loss, preeclampsia, gestational diabetes mellitus and infection/inflammation is discussed.

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The trophectoderm acts as a niche for the inner cell mass through C/EBPα-regulated IL-6 signaling

Cited by 7 publications

References 45 publications

CEBPA Phase Separation Links Transcriptional Activity and 3D Chromatin Hubs

CEBPA Phase Separation Links Transcriptional Activity and 3D Chromatin Hubs

The pro‐inflammatory signature of lipopolysaccharide in spontaneous contracting embryoid bodies differentiated from mouse embryonic stem cells

IL-6 and IL-8: An Overview of Their Roles in Healthy and Pathological Pregnancies

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