The triple role of glutathione S-transferases in mammalian male fertility

Llavanera, Marc; Mateo‐Otero, Yentel; Bonet, Sergi; Barranco, Isabel; Fernández-Fuertes, Beatriz; Yeste, Marc

doi:10.1007/s00018-019-03405-w

Cited by 32 publications

(25 citation statements)

References 102 publications

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“…Given the role of sperm GSTM3 as a sperm quality [18], fertility [29] and cryotolerance [30] biomarker and its extracellular membrane-attached localization in mammalian species (reviewed in Reference [17]), SP-GSTM3 is likely to be related to those sperm parameters. Therefore, it is reasonable to suggest that exploring SP-GSTM3 as a sperm quality and in vivo fertility biomarker may improve the evaluation of reproductive performance of pig AI-doses.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, it is worth bearing in mind that the class-clustered organization of GST genes in both plant and animals reveals their importance during evolutionary history. Furthermore, GSTs are ubiquitous and highly conserved enzymes among species (reviewed from Reference [17]). Therefore, the fact that GSTs are highly conserved proteins would support the similar GSTM3 concentrations found in the SP of these three pig breeds.…”

Section: Discussionmentioning

confidence: 99%

“…Glutathione S-transferases (GSTs) are essential antioxidant enzymes involved in cellular protection against oxidative stress, preserving sperm function and fertilizing ability (reviewed in Reference [17]). Antioxidant enzymes are known to be especially relevant for sperm cells since they are highly sensitive to oxidative stress due to the high amount of polyunsaturated fatty acids and their relatively low antioxidant capacity.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies in pig and goat sperm evidenced the significance of GSTs in mammalian sperm physiology, highlighting their role on preserving mitochondrial function and maintaining plasma membrane stability [18,19]. Recently, the triple role of sperm GSTs has been well-established, being involved in cell detoxification, cellular signaling regulation and sperm-zona pellucida binding events [17]. In addition, previous research, including three recent meta-analysis, confirmed that some GSTs null-genotypes are a risk factor for male idiopathic subfertility or infertility in men [20][21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, sperm GST Mu 3 (GSTM3) has been recently proposed as a quality [18], fertility [29] and cryotolerance [30] biomarker for pig sperm. Mounting evidence demonstrates the extracellular membrane-attached localization of sperm GSTs (reviewed from Reference [17]), surmising a potential direct relationship between sperm and SP-GSTM3. Therefore, SP-GSTM3 is a promising candidate to sperm quality and fertility biomarker.…”

Section: Introductionmentioning

confidence: 99%

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Exploring Seminal Plasma GSTM3 as a Quality and In Vivo Fertility Biomarker in Pigs—Relationship with Sperm Morphology

et al. 2020

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Glutathione S-transferases Mu 3 (GSTM3) is an essential antioxidant enzyme whose presence in sperm has recently been related to sperm cryotolerance, quality and fertility. However, its role in seminal plasma (SP) as a predictor of the same sperm parameters has never been investigated. Herein, cell biology and proteomic approaches were performed to explore the presence, origin and role of SP-GSTM3 as a sperm quality and in vivo fertility biomarker. GSTM3 in SP was quantified using a commercial Enzyme-Linked Immunosorbent Assay (ELISA) kit specific for Sus scrofa, whereas the presence of GSTM3 in testis, epididymis and accessory sex glands was assessed through immunoblotting analysis. Sperm quality and functionality parameters were evaluated in semen samples at 0 and 72 h of liquid-storage, whereas fertility parameters were recorded over a 12-months as farrowing rate and litter size. The presence and concentration of GSTM3 in SP was established for the first time in mammalian species, predominantly synthesized in the epididymis. The present study also evidenced a relationship between SP-GSTM3 and sperm morphology and suggested it is involved in epididymal maturation rather than in ejaculated sperm physiology. Finally, the data reported herein ruled out the role of this antioxidant enzyme as a quality and in vivo fertility biomarker of pig sperm.

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