The Trichoderma cellulase regulatory puzzle: From the interior life of a secretory fungus

Kubicek, Christian P.; Messner, Rudolf; Gruber, Franz; Mach, Robert L.; Kubicek-Pranz, E.M.

doi:10.1016/0141-0229(93)90030-6

Cited by 173 publications

(83 citation statements)

References 111 publications

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“…The optimum temperature for activity of extracellular enzyme activities was 60˚C and 45˚C for polygalacturo- (Figure 5), and these results are consistent with those reported for the fungi C. lindemuthianum [26], Neurospora crassa and Penicillium sp [27], and Trichoderma reesei [28]. It has been suggested that the temperature response for different types of extracellular lytic enzymes, is that as isoenzymes found in different species, with different properties [27].…”

Section: Effect Of Temperature and Protein Concentrationsupporting

confidence: 81%

Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

Moctezuma-Zárate¹,

Vargas-Morales²,

González³

et al. 2013

AiM

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Fusarium solani is a necrotrophic parasitic fungus that causes wilt in some plants, causing severe economic losses in some areas of the country. The objective of this work was to analyze the induction of extracellular lytic enzymes produced by a strain of F. solani, isolated from a culture of tomato, in Villa de Arista, S.L.P. México. Polygalacturonase activity has a greater induction time at 10 days, and the xylanase has two times higher activity at 8 and 13 days of incubation at 28˚C. Also, the xylanase activities A and B were very stable at 4˚C. After 7 days of incubation, it has an activity of 100% and 96%, respectively, while polygalacturonase retains 61% of its initial activity. Both activities are better induced with glutamate and urea as nitrogen sources respectively, and both exhibit an initial pH optimum of 5.5. Finally, we didn't find cellulase activity in the analyzing conditions.

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Section: Effect Of Temperature and Protein Concentrationsupporting

confidence: 81%

Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

Moctezuma-Zárate¹,

Vargas-Morales²,

González³

et al. 2013

AiM

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“…However, the use of insoluble substrates to induce enzyme secretion is not ideal for industrial processes. Because these naturally inducing substances cannot enter fungal cells, it is believed that oligosaccharides released from polymers and their derivatives function as the actual molecules that trigger enzyme induction (4). Cellobiose, the major soluble end product of cellulases, moderately induces cellulase gene expression and activity in Hypocrea jecorina (Trichoderma reesei) (5-7) and Aspergillus species (8), which are commonly used fungi for high-level enzyme production (9).…”

mentioning

confidence: 99%

Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins

Znameroski¹,

Coradetti

Roche³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

210

212

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Neurospora crassa colonizes burnt grasslands in the wild and metabolizes both cellulose and hemicellulose from plant cell walls. When switched from a favored carbon source such as sucrose to cellulose, N. crassa dramatically upregulates expression and secretion of a wide variety of genes encoding lignocellulolytic enzymes. However, the means by which N. crassa and other filamentous fungi sense the presence of cellulose in the environment remains unclear. Here, we show that an N. crassa mutant carrying deletions of two genes encoding extracellular β-glucosidase enzymes and one intracellular β-glucosidase lacks β-glucosidase activity, but efficiently induces cellulase gene expression in the presence of cellobiose, cellotriose, or cellotetraose as a sole carbon source. These data indicate that cellobiose, or a modified version of cellobiose, functions as an inducer of lignocellulolytic gene expression in N. crassa . Furthermore, the inclusion of a deletion of the catabolite repressor gene, cre-1 , in the triple β-glucosidase mutant resulted in a strain that produces higher concentrations of secreted active cellulases on cellobiose. Thus, the ability to induce cellulase gene expression using a common and soluble carbon source simplifies enzyme production and characterization, which could be applied to other cellulolytic filamentous fungi.

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“…For rapid launching of the cellulase machinery, T. reesei has to sense the presence of the insoluble polysaccharide cellulose outside the cell (2)(3)(4)(5). Although the identity of the true "inducer" and the ensuing intracellular inductor cascade is still speculative, several lines of evidence have been presented suggesting that the soluble oligosaccharides released from cellulose or their derivatives may well function as the actual molecules that trigger cellulase induction (4). Specifically, both cellobiose, the major soluble end product of cellulose, and its transglycosylating derivative, sophorose, have been shown to induce cellulase gene expression and activity in T. reesei (3, 6 -8).…”

mentioning

confidence: 99%

Two Major Facilitator Superfamily Sugar Transporters from Trichoderma reesei and Their Roles in Induction of Cellulase Biosynthesis

Zhang

Kou

et al. 2013

Journal of Biological Chemistry

134

202

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Background:The molecular mechanism underlying the regulation of cellulase production by T. reesei is unclear. Results: The absence of sugar transporter Stp1 enhanced cellulase gene induction whereas the absence of Crt1 abolished cellulase gene expression. Conclusion: Crt1 is essential in cellulase gene induction independent of intracellular sugar delivery. Significance: These data shed light on the mechanism by which T. reesei senses and transmits cellulose signal.

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The Trichoderma cellulase regulatory puzzle: From the interior life of a secretory fungus

Cited by 173 publications

References 111 publications

Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins

Two Major Facilitator Superfamily Sugar Transporters from Trichoderma reesei and Their Roles in Induction of Cellulase Biosynthesis

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The Trichoderma cellulase regulatory puzzle: From the interior life of a secretory fungus

Cited by 173 publications

References 111 publications

Induction of Extracellular Lytic Enzymes by &lt;i&gt;Fusarium solani&lt;/i&gt;

Induction of Extracellular Lytic Enzymes by &lt;i&gt;Fusarium solani&lt;/i&gt;

Induction of lignocellulose-degrading enzymes in Neurospora crassa by cellodextrins

Two Major Facilitator Superfamily Sugar Transporters from Trichoderma reesei and Their Roles in Induction of Cellulase Biosynthesis

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Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>