The Trichoderma brevicompactum clade: a separate lineage with new species, new peptaibiotics, and mycotoxins

Degenkolb, Thomas; Dieckmann, Ralf; Nielsen, Kristian Fog; Gräfenhan, Tom; Theis, C.; Zafari, Doustmorad; Chaverri, Priscila; Ismaiel, Adnan; Brückner, Hans; Döhren, Hans von; Thrane, Ulf; Petrini, Orlando; Samuels, Gary J.

doi:10.1007/s11557-008-0563-3

Cited by 139 publications

(142 citation statements)

References 71 publications

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“…The same primer sets used for PCR amplification were used to sequence both DNA strands. In the case of rpb2 gene, two additional internal primers, RPB-432F and RPB-450R (Degenkolb et al, 2008), were used for the sequencing reactions. Gel electrophoresis and data collection were performed on an ABI Prism 310 Genetic Analyzer (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

Kim¹,

Park²,

Kim³

et al. 2012

The Plant Pathology Journal

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During investigating of shiitake mushroom competitors, 289 isolates of Trichoderma spp. were collected from shiitake mushroom farms in different districts and the Forest Mushroom Research Center of Korea, among which 29 representative strains were selected. Based on the DNA sequences of the rpb2 and tef1 genes and the ITS rDNA, and their morphological characteristics, they were identified as T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, and two undescribed species, Trichoderma spp. 1 and 2, which are considered to be the candidate of new species. Competition tests between Lentinula edodes (Sanjo302) and the Trichoderma species indicated that the six species of Trichoderma were significantly different from each other in terms of their ability to invade the mycelial blocks of shiitake. In both of dual cultures on potato dextrose agar and sawdust media, Trichoderma spp. 1 and 2 strongly invaded the mycelial blocks of shiitake. Our results suggest that the two Trichoderma species may cause potentially serious economic losses in shiitake cultivation of Korea.

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Section: Methodsmentioning

confidence: 99%

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

Kim¹,

Park²,

Kim³

et al. 2012

The Plant Pathology Journal

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“…Trichothecenes are produced by the genera Fusarium, Stachybotris, Myrothecium, and Trichoderma (14) as well as others (12). The trichothecene biosynthetic pathways in Trichothecium (33), Fusarium (5,8,23,26,29,59), Myrothecium (53), and, recently, Trichoderma (11) have been studied.…”

mentioning

confidence: 99%

Involvement of Trichoderma Trichothecenes in the Biocontrol Activity and Induction of Plant Defense-Related Genes

Malmierca

Cardoza

Alexander

et al. 2012

Appl Environ Microbiol

146

129

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cTrichoderma species produce trichothecenes, most notably trichodermin and harzianum A (HA), by a biosynthetic pathway in which several of the involved proteins have significant differences in functionality compared to their Fusarium orthologues. In addition, the genes encoding these proteins show a genomic organization differing from that of the Fusarium tri clusters. Here we describe the isolation of Trichoderma arundinaceum IBT 40837 transformants which have a disrupted or silenced tri4 gene, a gene encoding a cytochrome P450 monooxygenase that oxygenates trichodiene to give rise to isotrichodiol, and the effect of tri4 gene disruption and silencing on the expression of other tri genes. Our results indicate that the tri4 gene disruption resulted in a reduced antifungal activity against Botrytis cinerea and Rhizoctonia solani and also in a reduced ability to induce the expression of tomato plant defense-related genes belonging to the salicylic acid (SA) and jasmonate (JA) pathways against B. cinerea, in comparison to the wild-type strain, indicating that HA plays an important function in the sensitization of Trichoderma-pretreated plants against this fungal pathogen. Additionally, the effect of the interaction of T. arundinaceum with B. cinerea or R. solani and with tomato seedlings on the expressions of the tri genes was studied.

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“…They are commonly found in soil and play an important role as decomposers of decaying plant material and insect pathogens (2). In addition, members of this genus are important biotechnologically due to their ability to produce a wide spectrum of bioactive compounds (3).…”

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confidence: 99%

Phylogeny of the Clinically Relevant Species of the Emerging Fungus Trichoderma and Their Antifungal Susceptibilities

et al. 2014

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A set of 73 isolates of the emerging fungus Trichoderma isolated from human and animal clinical specimens were characterized morphologically and molecularly using a multilocus sequence analysis that included the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA and fragments of the translation elongation factor 1 alpha (Tef1), endochitinase CHI18-5 (Chi18-5), and actin 1 (Act1) genes. The most frequent species was Trichoderma longibrachiatum (26%), followed by Trichoderma citrinoviride (18%), the Hypocrea lixii/Trichoderma harzianum species complex (15%), the newly described species Trichoderma bissettii (12%), and Trichoderma orientale (11%). The most common anatomical sites of isolation in human clinical specimens were the respiratory tract (40%), followed by deep tissue (30%) and superficial tissues (26%), while all the animal-associated isolates were obtained from superficial tissue samples. Susceptibilities of the isolates to eight antifungal drugs in vitro showed mostly high MICs, except for voriconazole and the echinocandins.

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The Trichoderma brevicompactum clade: a separate lineage with new species, new peptaibiotics, and mycotoxins

Cited by 139 publications

References 71 publications

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

Involvement of Trichoderma Trichothecenes in the Biocontrol Activity and Induction of Plant Defense-Related Genes

Phylogeny of the Clinically Relevant Species of the Emerging Fungus Trichoderma and Their Antifungal Susceptibilities

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