The Trichinella spiralis-derived antigens alleviate HFD-induced obesity and inflammation in mice

Tong, Mingwei; Yang, Xiaodan; Liu, Haixia; Ge, Huihui; Huang, Guangrong; Kang, Xing; Yang, Hao; Liu, Qingqing; Ren, Ping; Kuang, Xiaoyu; Yan, Huan; Shen, Xiaorong; Qiao, Yuyu; Kang, Yongbo; Li, Lin; Yang, Yong; Fan, Weiping

doi:10.1016/j.intimp.2023.109924

Cited by 3 publications

(7 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our previous study showed that TsAg exhibits therapeutic effects on obesity through the gut−fat axis in an HFD mouse model. 17 Considering that NAFLD is closely associated with obesity, we speculate that TsAg could also alleviate NAFLD. In the present study, we identify a novel role of TsAg, which shows potential alleviating effects on NAFLD, confirming our hypothesis.…”

Section: ■ Discussionmentioning

confidence: 93%

“…For example, TsAg treatment increased the abundance of Rikenellaceae, Ruminococcaceae, Lachnospiraceae, and Akkermansiaceae but decreased the abundance of Erysipelotrichaceae and Eggerthellaceae. 17 Ruminococcaceae and Rikenellaceae levels have been demonstrated to be reduced in NAFLD. 49 Bacteria belonging to the family Lachnospiraceae have been shown to produce short-chain fatty acids, thus, exerting a protective role in alleviating obesity, 50,51 modulating gut inflammation, 52 and maintaining intestinal integrity.…”

Section: ■ Discussionmentioning

confidence: 99%

“…In our previous study, TsAg was found to be an effective inducer in regulating HFD-induced gut dysbiosis, characterized by the upregulation of richness of beneficial bacteria, as well as downregulation of harmful bacteria. For example, TsAg treatment increased the abundance of Rikenellaceae , Ruminococcaceae , Lachnospiraceae, and Akkermansiaceae but decreased the abundance of Erysipelotrichaceae and Eggerthellaceae .…”

Section: Discussionmentioning

confidence: 99%

“…TsAg was prepared as previously reported. 17 In brief, ML from the mice infected with T. spiralis (ISS534) were obtained at 35 days post-infection (dpi). After washing several times with phosphate-buffered saline (PBS), ML of T. spiralis resuspended in PBS were homogenized using a tissue homogenizer, and then was frozen in liquid nitrogen.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…spiralis-derived antigens with a soluble portion of somatic antigens, known as crude worm extracts (CWE), excretory–secretory (ES) products, and recombinant proteins result in immune regulation and protection against various inflammatory diseases including airway disease, inflammatory bowel disease, multiple sclerosis, and other inflammatory diseases . Notably, our previous study reported that TsAg could partially alleviate HFD-induced obesity in mice by regulating intestinal microbiota . In addition, a recent epidemiological study demonstrated that a previous schistosome infection negatively correlated with fatty liver .…”

mentioning

confidence: 99%

See 4 more Smart Citations

Effect of Trichinella spiralis-Derived Antigens on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice

Yang,

He,

Yang

et al. 2024

ACS Pharmacol. Transl. Sci.

Self Cite

View full text Add to dashboard Cite

Nonalcoholic fatty liver disease (NAFLD) is a liver disease characterized by hepatic steatosis, inflammation, and fibrosis, as well as gut dysbiosis. No approved effective therapeutic medicine is available to date for NAFLD. Helminth therapy is believed to be a novel direction and therapeutic strategy for NAFLD. Our previous study showed that Trichinella spiralis-derived antigens (TsAg) had the potential for partially alleviating obesity via regulating gut microbiota. However, the effect of TsAg on NAFLD remains unclear. In this study, high-fat diet (HFD)-induced model mice were treated with TsAg and microbiota transplantation experiments, and alterations in the pathogenesis of nonalcoholic liver disease were assessed. The results showed that TsAg markedly reduced hepatic steatosis, improved insulin resistance, and regulated the abnormal expression of hepatic lipid-related genes. Of note, TsAg ameliorated hepatic inflammation by decreasing proinflammatory TNF-α and IL-1β, suppressing hepatic macrophage infiltration, as well as promoting M2 macrophage polarization. Moreover, TsAg reversed gut dysbiosis, as especially indicated by an increase in beneficial bacteria (e.g., Akkermansiaceae and Rikenellaceae). Furthermore, our study found that TsAg reduced LPS hepatic translocation and hepatic TLR4/NF-κB signaling, which further contributed to inhibiting hepatic inflammation. In addition, TsAg inhibited hepatic oxidative stress involving Nrf2/NQO-1 signaling. Microbiota transplantation showed that TsAg-altered microbiota is sufficient to confer protection against NAFLD in HFDinduced mice. Overall, these findings suggest that TsAg involving gut−liver axis and Nrf2/NQO-1 signaling is a novel promising candidate for NAFLD treatment. TsAg restores intestinal microbiota and intestinal barrier to inhibit bacteria and LPS translocation into the liver, contributing to reduce inflammation, oxidative stress, and hepatic steatosis in the liver of NAFLD mice. The effects were attributed to, at least in part, the inactivation of NF-κB pathway and the activation of Nrf-2/NQO-1 pathway. This study provides new insights for understanding immune modulation by T. spiralis-derived products as well as the potential application of TsAg as a modality for NAFLD.

show abstract

Section: ■ Discussionmentioning

confidence: 93%

Section: ■ Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Effect of Trichinella spiralis-Derived Antigens on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice

Yang,

He,

Yang

et al. 2024

ACS Pharmacol. Transl. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

Serine protease inhibitor from the muscle larval Trichinella spiralis ameliorates non-alcoholic fatty liver disease in mice via anti-inflammatory properties and gut-liver crosstalk

Tong,

Yang,

Qiao

et al. 2024

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

Transcriptome profiling of A549 non-small cell lung cancer cells in response to Trichinella spiralis muscle larvae excretory/secretory products

Wang,

Zhu,

et al. 2023

Front. Vet. Sci.

View full text Add to dashboard Cite

Trichinella spiralis (T. spiralis) muscle-larva excretory/secretory products (ML-ESPs) is a complex array of proteins with antitumor activity. We previously demonstrated that ML-ESPs inhibit the proliferation of A549 non-small cell lung cancer (NSCLC) cell line. However, the mechanism of ML-ESPs against A549 cells, especially on the transcriptional level, remains unknow. In this study, we systematically investigated a global profile bioinformatics analysis of transcriptional response of A549 cells treated with ML-ESPs. And then, we further explored the transcriptional regulation of genes related to glucose metabolism in A549 cells by ML-ESPs. The results showed that ML-ESPs altered the expression of 2,860 genes (1,634 upregulated and 1,226 downregulated). GO and KEGG analysis demonstrated that differentially expressed genes (DEGs) were mainly associated with pathway in cancer and metabolic process. The downregulated genes interaction network of metabolic process is mainly associated with glucose metabolism. Furthermore, the expression of phosphofructokinase muscle (PFKM), phosphofructokinase liver (PFKL), enolase 2 (ENO2), lactate dehydrogenase B (LDHB), 6-phosphogluconolactonase (6PGL), ribulose-phosphate-3-epimerase (PRE), transketolase (TKT), transaldolase 1 (TALDO1), which genes mainly regulate glycolysis and pentose phosphate pathway (PPP), were suppressed by ML-ESPs. Interestingly, tricarboxylic acid cycle (TCA)-related genes, such as pyruvate dehydrogenase phosphatase 1 (PDP1), PDP2, aconitate hydratase 1 (ACO1) and oxoglutarate dehydrogenase (OGDH) were upregulated by ML-ESPs. In summary, the transcriptome profiling of A549 cells were significantly altered by ML-ESPs. And we also provide new insight into how ML-ESPs induced a transcriptional reprogramming of glucose metabolism-related genes in A549 cells.

show abstract

The Trichinella spiralis-derived antigens alleviate HFD-induced obesity and inflammation in mice

Cited by 3 publications

References 69 publications

Effect of Trichinella spiralis-Derived Antigens on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice

Effect of Trichinella spiralis-Derived Antigens on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice

Serine protease inhibitor from the muscle larval Trichinella spiralis ameliorates non-alcoholic fatty liver disease in mice via anti-inflammatory properties and gut-liver crosstalk

Transcriptome profiling of A549 non-small cell lung cancer cells in response to Trichinella spiralis muscle larvae excretory/secretory products

Contact Info

Product

Resources

About