The Trend in Established Analytical Techniques in the Investigation of Physicochemical Properties and Various Constituents of Honey: a Review

Manickavasagam, Ganapaty; Saaid, Mardiana; Osman, Rozita

doi:10.1007/s12161-022-02356-6

Cited by 10 publications

(7 citation statements)

References 198 publications

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“…Similarly, other tropical climate countries like Thailand and Indonesia also set their maximum moisture content level of honey higher than international standards; 27.5% in Indonesia and 21.0% in Thailand (ACFS, 2014; SNI, 2018). However, when the moisture content of honey samples is high, it will trigger the growth of microorganisms, which eventually lead to fermentation and shorten the shelf‐life of honey (Manickavasagam et al., 2022; Saidan et al., 2020). The moisture content of H. itama honey from Malaysia has been reported as 30.8% and 22.50%–27.87% (Julika et al., 2022; Ng et al., 2021).…”

Section: Resultsmentioning

confidence: 99%

“…All 45 samples from three different origins have 5‐HMF content within the maximum limit set by Malaysian Standard, Codex Alimentarius Standard, and EU Honey Directive. High 5‐HMF content is detrimental to human health as it possesses cytotoxicity, genotoxicity, and mutagenicity (Manickavasagam et al., 2022). As the 5‐HMF content from this study is within the optimum limits, it is safe to consume.…”

Section: Resultsmentioning

confidence: 99%

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Quality assessment and chemometrics application on physicochemical characteristics, antioxidant properties, and 5‐HMF content of Malaysian stingless bee honey from different topographical origins

Manickavasagam

Saaid

Lim

et al. 2023

Journal of Food Science

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The popularity of Malaysian stingless bee honey is rising among healthconscious individuals; thus, chemical and physical evaluations of Malaysian stingless bee honey are vital to ensure the honey has achieved the optimum limits set by Malaysian and international regulatory standards so that it can be commercialized locally and internationally. Therefore, in the present study, the physicochemical characteristics (moisture content, total dissolved solids, pH, free acidity, electrical conductivity, and ash content), antioxidant properties (total phenolic and flavonoid contents), and 5-hydroxymethylfurfural (5-HMF) of Heterotrigona itama (H. itama) honey from different sites in Peninsular Malaysia were investigated. Subsequently, the correlation between these chemical and physical parameters was studied using Spearman correlation coefficients. The significant difference between H. itama honey from different topographical origins was studied using univariate analysis (one-way ANOVA followed by post hoc Tukey's test). The discrimination pattern of 45 honey samples based on their topographical origins was evaluated using cluster analysis (heatmap and dendrogram) and chemometrics analysis (partial least squares-discriminant analysis). Results showed that some samples of certain parameters (electrical conductivity, free acidity, and moisture content) have exceeded the limit set by the international regulatory standard. However, the 5-HMF content of all samples was within the allowed range. A statistically significant difference (p < 0.05) has been observed for all the parameters except electrical conductivity and ash content in terms of inter-topographical origins. Although the profiles of H. itama honey from different origins were close, most of them were separated according to their topographical origins and were validated using a permutation test.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Quality assessment and chemometrics application on physicochemical characteristics, antioxidant properties, and 5‐HMF content of Malaysian stingless bee honey from different topographical origins

Manickavasagam

Saaid

Lim

et al. 2023

Journal of Food Science

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“…Proline percentage of honey is one of the important criteria used to determine the quality of honey, as it provides information about the antioxidant activity of honey, its maturity and sugar adulteration in honey. In general, there are three common spectrophotometric methods [5,10–11] used to determine the proline content of honey [14] . However, the total sample processing times applied for the extraction in these three methods differ significantly.…”

Section: Resultsmentioning

confidence: 99%

“…In general, there are three common spectrophotometric methods [5,[10][11] used to determine the proline content of honey. [14] However, the total sample processing times applied for the extraction in these three methods differ significantly. However, some process temperatures applied in the extraction stages also show differences.…”

Section: Effect Of Extraction Conditions On Proline Contentmentioning

confidence: 99%

Utilization of Response Surface Methodology in Optimization of Proline Extraction from Castanea sativa Honey

Bayram¹,

Kutlu²,

Gerçek

2023

Chemistry & Biodiversity

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Proline constitutes approximately 85 % of the amino acid composition of honey. Therefore, the quantitative determination of this amino acid in honey samples is used by many national/international authorities to evaluate the quality of honey types. In this study, it was aimed to achieve maximum proline amino acid extraction from honey samples whose botanical origins were confirmed by melissopalynological analysis. For this reason, based on three different spectrophotometric methods used in the literature for proline analysis, proline extraction was optimized with the Response Surface Method (RSM) and Box-Behnken experimental design. Three independent variables were determined as treatment time (2, 6, and 10 min), treatment temperature (22, 46, and 70 °C), and cooling time (5, 25, and 45 min). As a result of the optimization, it was seen that only significantly effective independent variable on the proline content of honey was the processing temperature. The optimum conditions obtained as a result of the RSM were found to be 2 min for the treatment time, 70 °C for the treatment temperature and 45 min for the cooling time. The composite desirability of the optimum conditions (R 2 ) was found to be 1.00. It was determined that the method proposed by International Honey Commission (IHC) is efficient for proline analysis, but it provides more proline extraction by reducing of time from 10 min to 2 min in hold time in boiling water bath only during the extraction step. As a result, the conditions to be used in order to achieve maximum proline extraction with different spectrophotometric methods were determined and optimum values were determined. In addition, since the botanical origin of honey samples significantly affects the proline content of honey, it can be suggested that this study be optimized for different monofloral honey samples as well.

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“…Yet, there are some exceptions in terms of minimum percentage because of the numerous over‐ or underrepresented pollen types; the pollen percentages can vary greatly (Von Der Ohe et al., 2004). In terms of species of honeybees, honey is called sting bee honey when sting bees are responsible for the nectar collection, whereas it is called stingless bee honey when stingless bees are accountable (Manickavasagam et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

Impact of prolonged storage on quality assessment properties and constituents of honey: A systematic review

Manickavasagam,

Saaid,

Lim

2024

Journal of Food Science

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This systematic review paper aims to discuss the trend in quality assessment properties and constituents of honey at different storage conditions and confer the possible whys and wherefores associated with the significant changes. Initially, a literature search was conducted through Google Scholar, ScienceDirect, PubMed, and Scopus databases. In total, 43 manuscripts published between 2001 and 2023 that met the inclusion and exclusion criteria were chosen for the review. As an outcome of this review, prolonged honey storage could deteriorate sensory, nutritional, and antioxidant properties and promote fermentation, granulation, microbial growth, carcinogenicity, organotoxicity, and nephrotoxicity. This systematic review also recognized that diastase activity, invertase activity, 5‐hydroxymethylfurfural content, proline content, sugar content, amino acids, and vitamins could be used as indicators to distinguish fresh and stored honey based on the significant test (p‐value) in the reported studies. However, all the reported studies used the simplest approach (one‐way ANOVA) to identify the significant differences in the analyzed parameter during the storage period and none of them reported an approach to identify the most influential parameter at different storage conditions. In conclusion, orthogonal partial least squares discriminant analysis (supervised multivariate statistical tool) has to be employed in future studies to find the most influential parameter and could be used to potent chemical markers to distinguish fresh and stored honey because this analysis is incorporated with S‐plot, variable importance of projection, and one‐way ANOVA, which can produce the most accurate and precise results rather solely depending on one‐way ANOVA.

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The Trend in Established Analytical Techniques in the Investigation of Physicochemical Properties and Various Constituents of Honey: a Review

Cited by 10 publications

References 198 publications

Quality assessment and chemometrics application on physicochemical characteristics, antioxidant properties, and 5‐HMF content of Malaysian stingless bee honey from different topographical origins

Quality assessment and chemometrics application on physicochemical characteristics, antioxidant properties, and 5‐HMF content of Malaysian stingless bee honey from different topographical origins

Utilization of Response Surface Methodology in Optimization of Proline Extraction from Castanea sativa Honey

Impact of prolonged storage on quality assessment properties and constituents of honey: A systematic review

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