The Treatment of Prednisone in Mild Diabetic Rats: Biochemical Parameters and Cell Response

Abstract

Background: Limited studies have been carried out with prednisone (PRED) in treatment by glucose intolerant individuals, even in this model the animals presented low blood glucose levels at adulthood, by the high regenerative capacity of β-cell. Objective: The aim was to evaluate the effects of the treatment of PRED in mild diabetes on biochemical and immunological biomarkers. Methods: Rats were randomly divided into four groups: control (C), treated control C+PRED (treatment of 1.25 mg/Kg/day PRED); diabetic DM (mild diabetes) and treated diabetic DM+PRED (treatment with same dose as C+PRED group). Untreated groups received vehicle, adjusted volume to body weight. The treatment lasted 21 days and measured body weight, food and water intake, and glycemia weekly. In the 3rd week, the Oral Glucose Tolerance Test (OGTT) and the Insulin Tolerance Test (ITT) was performed. On the last day, the rats were killed and the blood was collected for biochemical analyzes, leukogram and immunoglobulin G levels. Results: There was a significant decrease in body weight in mild diabetes; however, the treatment in diabetic groups increased food intake, glycemia, and the number of total leukocytes, lymphocytes and neutrophils. On the other hand, it decreased the levels of triglycerides, high-density and very lowdensity lipoproteins. In addition, diabetic groups showed glucose intolerance and mild insulin resistance, confirming that this model induces glucose intolerant in adult life. Conclusion: The results showed that the use of prednisone is not recommended for glucose intolerant individuals and should be replaced in order to not to aggravate this condition.

Gastric plication surgery changes gastrointestinal and metabolic parameters in an obesity‐induced high‐fat diet model

“…From then on, the animals received 2.0 g/kg glucose solution (200.0 g/L) by gavage. Blood glucose was determined at 30, 60, and 120 min after glucose solution administration 31 . The glycemic response during OGTT was assessed by the total estimate given by area under the curve (AUC) 32 .…”

“…Blood glucose was determined at 30, 60, and 120 min after glucose solution administration. 31 The glycemic response during OGTT was assessed by the total estimate given by area under the curve (AUC). 32 Variations in the blood glucose levels were expressed as percent changes relative to the baseline for each animal.…”

Congenital Anomalies Programmed by Maternal Diabetes and Obesity on Offspring of Rats

“…To reproduce maternal hyperglycemia found in Type 2 DM in animal models, streptozotocin (STZ) induction can be performed in the neonatal period of rats (Tsuji et al, 1988 ; Jawerbaum and White, 2010 ; Santos et al, 2015 ; Bequer et al, 2018 ; Bueno et al, 2020 ). This type of experimental diabetes is termed as “mild diabetes” (Hauschildt et al, 2018 ; Machado et al, 2020 ). Besides diabetic status, a change in lifestyle, especially in dietary patterns related with growing consumption of industrialized foods (high in calories and fat), affects diabetic progress at long term (Hu, 2011 ; Ley et al, 2014 ; Popkin, 2015 ; Krishan et al, 2018 ).…”

Maternal‐fetal outcomes of exercise applied in rats with mild hyperglycemia after embryonic implantation

“…These results may be due to an exacerbated hyperglycemia or exercise frequency, duration, and/or intensity. In view of the above, we used a diabetic rat model induced by neonatal STZ that replicates a hyperglycemic status similar to Type 2 DM in humans (Capobianco et al, 2016; Machado, Schavinski, Deluque, Volpato, & Campos, 2020), and a different less intense physical exercise protocol in pregnant rats to assess the maternal organism and embryo‐fetal development.…”