The treatment of bladder cancer in a mouse model by epigallocatechin-3-gallate-gold nanoparticles

Hsieh, Dar-Shih; Wang, Hang; Tan, Shan-Wen; Huang, Yi-Huei; Tsai, Cheng-Yuh; Yeh, Ming–Kung; Wu, Changshan

doi:10.1016/j.biomaterials.2011.06.073

Cited by 122 publications

(97 citation statements)

References 33 publications

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“…The EGCG-pNG particles at a ratio of 23:2.5 contained 27% EGCG conjugate, were around 50 nm in size, and had a zeta potential of -8 mV; these particles were used for further study. In our previous report, 9 EGCG particles were shown to be physically attached to the surface of pNG particles. In general, particle aggregation is less likely to occur for charged particles with an optimum zeta potential (±30 mV) due to electrostatic repulsion.…”

Section: Evaluation Of Egcg-loaded Pngmentioning

confidence: 86%

“…6 Some studies have demonstrated that EGCG combined with metal ions such as Fe 3+ or Mn 2+ to form complexes by gallate ring may enhance biological activity. 7,8 However, no studies have been conducted on EGCG-conjugated nanogold, with the exception of our previous study, 9 which reported that EGCG particles were physically attached onto the surface of physical nanogold (pNG) particles, confirmed by scanning electron microscopy. The anticancer activity of the EGCG-adsorbed pNG was investigated and confirmed to inhibit tumor cell growth by means of cell apoptosis.…”

Section: Introductionmentioning

confidence: 84%

“…Chen et al reported the intracellular accumulation of EGCG-conjugated AuNPs was due to AuNP-mediated endocytosis. 22 In our previous report, 9 we investigated the mechanism of EGCG-and pNG-mediated cell apoptosis. We showed that MBT-2 cells treated with EGCG or pNG were arrested in the S phase of the cell cycle.…”

mentioning

confidence: 99%

“…EGCG-pNG was confirmed to inhibit tumor cell growth by means of cell apoptosis. 9 The mechanism by which EGCG-pNG mediated tumor apoptosis was demonstrated to involve activation of the caspase cascade, via the Bcl-family proteins, of the mitochondrial pathway. Additionally, the mechanism by which tumors were suppressed following injection of EGCG-pNG directly into the tumor site was determined to be through downregulation of vascular endothelial growth factor, whereas the effect following oral administration of EGCG was through reversing immune suppression upon cancer progression.…”

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confidence: 99%

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The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Hsieh

Lu²,

Chen³

et al. 2012

IJN

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Nanogold particles are commonly used in nanomedicine. We generated physical nanogold (pNG) conjugated with different ratios of epigallocatechin-3-gallate (EGCG) and evaluated its physicochemical properties, antioxidant activity, and cytotoxicity in vitro as well as anticancer activity in vivo. Results showed that the EGCG-pNG conjugates were successfully prepared at ratios between 23:1 and 23:5, with the percentage of EGCG content increasing with the EGCG:pNG ratio from 23:1 (2.0% ± 0.02%) to 23:5 (28% ± 0.3%). EGCG-pNG particles at ratios of 23:1 and 23:5 demonstrated significantly decreased size from 500 to 20 nm and decreasing zeta potentials of 21 mV to -22 mV, respectively. At a ratio of 23:2.5, the EGCGpNG particles (27% EGCG, 50 nm in size, zeta potential of -8 mV) showed longer EGCG activity half-life (110 days vs 5 hours), controlled release (2 hours vs 30 minutes), and higher antioxidant activity (four times), as well as inhibition of tumor cell growth, than controls. The present study indicated that EGCG-pNG possesses promising therapeutic potential, based on its strong free-radical scavenging and anticancer activities.

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Section: Evaluation Of Egcg-loaded Pngmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 84%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Hsieh

Lu²,

Chen³

et al. 2012

IJN

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“…In a study of mice with subcutaneously implanted bladder cancer cells, epigallocatechin gallate exhibited proapoptotic properties, thus impeding cancer growth. The apoptotic process in this case is thought to involve the activation of a caspase cascade regulated by proteins from the BcL-2 family [46]. A study on human bladder cancer cells also confirmed the antiproliferative properties of epigallocatechin gallate, and polyphenon 60, epicatechin gallate, and the green tea extract were shown to have similar proapoptotic effects [44].…”

Section: Dietary Polyphenolsmentioning

confidence: 71%

Bladder cancer prevention

Długosz

Królik²

2017

Nowotwory. Journal of Oncology

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Plant‐Inspired Pyrogallol‐Containing Functional Materials

Shin

Park

Lee

2019

Adv Funct Materials

143

106

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Pyrogallol-containing molecules are ubiquitous in the plant kingdom. The chemical synthesis of these molecules remains challenging. Thus, they are obtained via purification from heterogeneous mixtures of plant extracts. Previous studies have focused on their biological roles, such as antioxidants. Additionally, the molecules are used as ink colorants and in tanning processes for leather. Recently, many disciplines have paid attention to adhesiveness of pyrogallol-containing molecules, including the control of interface properties in energy storage/generation and medical devices, as well as the changes in wettability related to membrane technologies. In particular, pyrogallol-containing molecules act as "molecular glues," binding to virtually all biomacromolecules, for example, DNA/RNA, soluble proteins, insoluble extracellular matrices, and peptides. Furthermore, the cohesion of pyrogallol by forming pyrogallol-to-pyrogallol covalent bonds is useful for the preparation of bulk hydrogels and thin films. The content of this review focuses on interactions with biomacromolecules used as molecular glues, used as modifiers in material-independent surface chemistry, and applied as chemical moieties to form covalent linkages to fabricate hydrogels and related biomaterials. Future perspectives include the development of new pyrogallol-containing materials, the understanding of chirality in adhesion, and the improvement of the mechanical stability for applications in various biomedical, energy, and industrial devices. molecules, strategies for surface modifications, and biomedical applications (Figure 1). Based on the timeline of pyrogallol research, we begin by describing the basic physicochemical properties of pyrogallol analogs, focusing on intermolecular interactions with macromolecules. Section 2 introduces the chemical versatility of pyrogallol analogs in macromolecular complexation, and analytical methods for the complexation are explained. Furthermore, the biological functions of pyrogallol analogs, particularly their therapeutic effects on cancers and inflammatory diseases, are explained. Next, surface modification strategies using pyrogallol-containing molecules are explained (Section 3). We discuss pyrogallol-derived formulations for biomedical purposes, ranging from hydrogels to particles (Section 4). Finally, we conclude with an outlook for the further development and implementation of natural pyrogallol analogs and pyrogallol-conjugated synthetic polymers. This review will be helpful for understanding past studies and current progress in pyrogallol-containing molecules research and for predicting future perspectives. Chemical and Structural Analysis of Colloidal ComplexationEarly uses largely involved intermolecular interactions between pyrogallol analogs and proteins. Pyrogallol analog complexations were particularly effective in studies of proline-rich proteins (PRPs)

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The treatment of bladder cancer in a mouse model by epigallocatechin-3-gallate-gold nanoparticles

Cited by 122 publications

References 33 publications

The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

The preparation and characterization of gold-conjugated polyphenol nanoparticles as a novel delivery system

Bladder cancer prevention

Plant‐Inspired Pyrogallol‐Containing Functional Materials

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