The transport of triterpenoids

Fang, Yubo; Xiao, Han

doi:10.1016/j.biotno.2021.03.001

Cited by 8 publications

(11 citation statements)

References 79 publications

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“…A total of 157 proteins exhibited significantly higher expression levels in the higher GA-HLDOA secreting-strain BJ5464-r than in strain INVSc1-r, and 153 proteins were only detected in BJ5464-r (Table S3). Our systematic review revealed that vesicle trafficking proteins, transporters, and cell wall- and cell membrane-related proteins are probably involved in the transport of triterpenoids . Of 300 proteins, 3 proteins related to vesicle trafficking, 3 transporters, 10 cell wall-related proteins, and 1 plasma membrane protein (17 proteins in total) were determined as candidates that affect the secretion of GA-HLDOA (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…The increasing demand for triterpenoids has necessitated their efficient bioproduction. Because the intracellular accumulation of triterpenoids often generates feedback inhibition on biosynthetic pathway enzymes, triterpenoid secretion is an effective approach for promoting triterpenoid bioproduction; this approach segregates triterpenoids from the intracellular environment, eliminating the cell lysis step, and thereby facilitating efficient triterpenoid extraction.…”

Section: Introductionmentioning

confidence: 99%

“…The monoterpenoids indole alkaloids and vincamine, the sesquiterpenes arteannuin B and artemisinin, and the diterpenoids labda-8(20),13-diene-15,16-diol, labda-7,13-diene-15,20-diol, and labda-7,13-diene-3,15-diol from Ceroplastes ceriferus can be secreted in extracellular environments; in contrast, most triterpenoids naturally accumulate inside the cells . Based on the production and storage processes of triterpenoids by natural hosts, we speculated that vesicles, transporters, and cell membrane/wall permeability are factors that affect triterpenoid secretion . During the production of the triterpenoid bryonolic acid, swellings of the rough endoplasmic reticulum are released into cytoplasm as vesicles, which eventually establish contact with the cell wall.…”

Section: Introductionmentioning

confidence: 99%

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The Aspartic Protease Yps3p and Cell Wall Glucanase Scw10p Are Novel Determinants That Enhance the Secretion of the Antitumor Triterpenoid GA-HLDOA in Saccharomyces cerevisiae

Fang

Xiao

2022

ACS Synth. Biol.

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Efficient bioproduction of triterpenoids is gaining increasing interest because of their significant biological applications; however, the secretion and bioproduction of triterpenoids are hindered by untapped genetic determinants. In our previous study, we observed that different engineered Saccharomyces cerevisiae strains exhibit different abilities for secreting the antitumor triterpenoid ganoderic acid 3-hydroxy-lanosta-8,24-dien-26-oic acid (GA-HLDOA). In the present study, we performed comparative proteomics analyses of the engineered strains and identified two genes, encoding an aspartic protease, YPS3, and a cell wall glucanase, SCW10, as the most effective determinants that enhance the secretion of GA-HLDOA. Compared with this control strain, strain BJ5464-r demonstrated an overexpression of YPS3 and SCW10 resulting in 3.9-fold and 4.7-fold higher secretion of GA-HLDOA, respectively, and these increases were accompanied by an increase in cell permeability. Moreover, compared with the YPS3-overexpressing strain, the SCW10-overexpressing strain had a thinner outer mannan layer. Our findings offer valuable insights into designing microbial cell factories for the efficient secretion of triterpenoids.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Aspartic Protease Yps3p and Cell Wall Glucanase Scw10p Are Novel Determinants That Enhance the Secretion of the Antitumor Triterpenoid GA-HLDOA in Saccharomyces cerevisiae

Fang

Xiao

2022

ACS Synth. Biol.

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“…Undoubtedly, however, a significant portion of terpenoids is transported between plant tissues in various ways after synthesis. The terpenoid transportation shall happen in at least three ways: transport in host cells, transport across the cell membrane, and intracellular transport ( Fang and Xiao, 2021 ). Terpenoids are usually extracted from different parts of plants such as roots, leaves, or fruits.…”

Section: Resultsmentioning

confidence: 99%

Comparative de novo transcriptome analysis of flower and root of Oliveria decumbens Vent. to identify putative genes in terpenes biosynthesis pathway

et al. 2022

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The Oliveria decumbens Vent. is a wild, rare, annual medicinal plant and endemic plant of Iran that has metabolites (mostly terpenes) which make it a precious plant in Persian Traditional Medicine and also a potential chemotherapeutic agent. The lack of genetic resources has slowed the discovery of genes involved in the terpenes biosynthesis pathway. It is a wild relative of Daucus carota. In this research, we performed the transcriptomic differences between two samples, flower and root of Oliveria decumbens, and also analyze the expression value of the genes involved in terpenoid biosynthesis by RNA-seq and its essential oil’s phytochemicals analyzed by GC/MS. In total, 136,031,188 reads from two samples of flower and root have been produced. The result shows that the MEP pathway is mostly active in the flower and the MVA in the root. Three genes of GPP, FPPS, and GGPP that are the precursors in the synthesis of mono, di, and triterpenes are upregulated in root and 23 key genes were identified that are involved in the biosynthesis of terpenes. Three genes had the highest upregulation in the root including, and on the other hand, another three genes had the expression only in the flower. Meanwhile, 191 and 185 upregulated genes in the flower and root of the plant, respectively, were selected for the gene ontology analysis and reconstruction of co-expression networks. The current research is the first of its kind on Oliveria decumbens transcriptome and discussed 67 genes that have been deposited into the NCBI database. Collectively, the information obtained in this study unveils the new insights into characterizing the genetic blueprint of Oliveria decumbens Vent. which paved the way for medical/plant biotechnology and the pharmaceutical industry in the future.

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“…It has been demonstrated that ABC transporters for triterpenoids exist. 84 With the initiation by binding of the triterpenoid to the TMD, after which a closed NBD dimer is formed, the triterpenoid is transported across the membrane. Subsequently, ATP hydrolysis triggers the dissociation of the closed NBD dimer; the TMD opens; and triterpenoids are secreted to the extracellular space.…”

Section: Its Glycosylated Derivatives From P Ginsengmentioning

confidence: 99%

Recent Advances in Yeast Recombinant Biosynthesis of the Triterpenoid Protopanaxadiol and Glycosylated Derivatives Thereof

Qiu

Blank

2023

J. Agric. Food Chem.

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Plant natural products are a seemingly endless resource for novel chemical structures. However, their extraction often results in high prices, fluctuation in both quantity and quality, and negative environmental impact. The latter might result from the extraction procedure but more often from the high amount of plant biomass required. With the advent of synthetic biology, producing natural plant products in large quantities using yeasts as hosts has become possible. Here, we focus on the recent advances in metabolic engineering of the yeasts species Saccharomyces cerevisiae and Yarrowia lipolytica for the synthesis of ginsenoside triterpenoids, namely, dammarenediol-II, protopanaxadiol, protopanaxatriol, compound K, ginsenoside Rh1, ginsenoside Rh2, ginsenoside Rg3, and ginsenoside F1. A discussion is provided on advanced synthetic biology, bioprocess strategies, and current challenges for the biosynthesis of ginsenoside triterpenoids. Finally, future directions in metabolic and process engineering are summarized and may help reify sustainable ginsenoside production.

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The transport of triterpenoids

Cited by 8 publications

References 79 publications

The Aspartic Protease Yps3p and Cell Wall Glucanase Scw10p Are Novel Determinants That Enhance the Secretion of the Antitumor Triterpenoid GA-HLDOA in Saccharomyces cerevisiae

The Aspartic Protease Yps3p and Cell Wall Glucanase Scw10p Are Novel Determinants That Enhance the Secretion of the Antitumor Triterpenoid GA-HLDOA in Saccharomyces cerevisiae

Comparative de novo transcriptome analysis of flower and root of Oliveria decumbens Vent. to identify putative genes in terpenes biosynthesis pathway

Recent Advances in Yeast Recombinant Biosynthesis of the Triterpenoid Protopanaxadiol and Glycosylated Derivatives Thereof

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