The transport of microspheres from the gastro-intestinal tract to inflammatory air pouches in the rat

Alpar, H. Oya; Field, W N; Hyde, Robert J.; Lewis, David

doi:10.1111/j.2042-7158.1989.tb06429.x

Cited by 77 publications

(22 citation statements)

References 6 publications

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“…This was roughly the same amount of time required for soluble dye tracers to cross the epithelium, indicating that the pathway has little resistance. Similar studies revealed that PLGA nanospheres were taken up in Peyer's patches after 1 h (Foster et al 1998) while uptake into the absorptive epithelium occurred within 30 min -1 h (Alpar et al 1990). …”

Section: Improving Uptake In the Gi Tractsupporting

confidence: 61%

Targeted nanoparticle-based drug delivery and diagnosis

Emerich

Thanos

2007

Journal of Drug Targeting

201

101

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Nanotechnology, or systems/device manufacture at sizes generally ranging between 1 and 100 nm, is a multidisciplinary scientific field undergoing explosive development. The genesis of nanotechnology can be traced to advances in medicine, communications, genomics and robotics. One of the greatest values of nanotechnology will be in the development of new and effective medical treatments (i.e. nanomedicine). This review focuses on the potential of nanomedicine as it relates to the development of nanoparticles for enabling and improving the targeted delivery of therapeutic and diagnostic agents. We highlight the use of nanoparticles for specific intra-compartmental analysis using the examples of delivery to malignant cancers, to the central nervous system, and across the gastrointestinal barriers.

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Section: Improving Uptake In the Gi Tractsupporting

confidence: 61%

Targeted nanoparticle-based drug delivery and diagnosis

Emerich

Thanos

2007

Journal of Drug Targeting

201

101

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“…Since, studies of PMNL phagocytosis of PNIPAm /MAA nanoparticles may provide information on the effects of serum factors on particle uptake, it was felt that HSA adsorption pro les may be more useful in assessing the effect of surface hydrophobicity on non-speci c particle uptake. We chose PMNLs and monocytes as model phagocytic cells, because the former is present in the blood whose phagocytic role is analogous to that of Kupffer cells in the liver [42], and the latter is able to derive to macrophages and often accumulate at in ammation sites such as rheumatoid arthritis [43].…”

Section: Introductionmentioning

confidence: 99%

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Moselhy

Nicholov

et al. 2000

Journal of Biomaterials Science, Polymer Edition

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The pH- and temperature-responsive poly(N-isopropylacrylamide-co-methacrylic acid) (PNIPAm/MAA) nanoparticles are of potential application in targeted drug delivery. Their responsive properties in the presence of human serum albumin were investigated using dynamic light scattering (DLS), protein assay, and electron spin resonance (ESR) spectroscopy. Their interaction with human monocytes and polymorphonuclear leukocytes (PMNLs) was studied using scanning electron microscopy (SEM) and oxygen consumption method. The nanoparticles exhibited a volume phase transition at 35-40 degrees C in Hanks balanced salt solution (HBSS) and in phosphate buffer solution (PBS) of pH 7.4. The diameter of the nanoparticles decreased slightly in the presence of HSA at 25 degrees C at neutral pH, whereas an increase in the diameter in pH 6 PBS at 40 degrees C was revealed. The amount of albumin adsorbed onto the nanoparticles decreased with increasing temperature. The ESR spectra of spin labeled HSA indicated a more restricted environment in the nanoparticles at elevated temperatures. The stimulation of PMNL oxygen consumption by PNIPAm based nanoparticles, an indication of phagocytosis of the particles, was not observed regardless whether the nanoparticles were incubated in plasma or serum. In contrast, the more hydrophobic polystyrene (PSt) particles induced a significant increase in the rate of oxygen consumption after the incubation. PNIPAm/MAA-grafted-PSt particles behaved similarly to the PNIPAm/MAA nanoparticles, suggesting that surface properties dictate the recognition of colloids by PMNLs.

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“…oral delivery | uptake mechanism B eginning in the 1960s, several groups (1)(2)(3)(4)(5)(6)(7) demonstrated that the small intestine could absorb microparticles with a diameter >1 μm, challenging dogma that the small intestine could only absorb small macromolecules. After this discovery, researchers began engineering microspheres (MSs) to deliver drugs with poor water solubility (8)(9)(10), poor gastrointestinal permeability (11), or poor oral bioavailability (8,9,(12)(13)(14)(15) to the small intestine.…”

mentioning

confidence: 99%

Unique insights into the intestinal absorption, transit, and subsequent biodistribution of polymer-derived microspheres

Reineke

Cho

Dingle

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Polymeric microspheres (MSs) have received attention for their potential to improve the delivery of drugs with poor oral bioavailability. Although MSs can be absorbed into the absorptive epithelium of the small intestine, little is known about the physiologic mechanisms that are responsible for their cellular trafficking. In these experiments, nonbiodegradable polystyrene MSs (diameter range: 500 nm to 5 μm) were delivered locally to the jejunum or ileum or by oral administration to young male rats. Following administration, MSs were taken up rapidly (≤5 min) by the small intestine and were detected by transmission electron microscopy and confocal laser scanning microscopy. Gel permeation chromatography confirmed that polymer was present in all tissue samples, including the brain. These results confirm that MSs (diameter range: 500 nm to 5 μm) were absorbed by the small intestine and distributed throughout the rat. After delivering MSs to the jejunum or ileum, high concentrations of polystyrene were detected in the liver, kidneys, and lungs. The pharmacologic inhibitors chlorpromazine, phorbol 12-myristate 13-acetate, and cytochalasin D caused a reduction in the total number of MSs absorbed in the jejunum and ileum, demonstrating that nonphagocytic processes (including endocytosis) direct the uptake of MSs in the small intestine. These results challenge the convention that phagocytic cells such as the microfold cells solely facilitate MS absorption in the small intestine.oral delivery | uptake mechanism B eginning in the 1960s, several groups (1-7) demonstrated that the small intestine could absorb microparticles with a diameter >1 μm, challenging dogma that the small intestine could only absorb small macromolecules. After this discovery, researchers began engineering microspheres (MSs) to deliver drugs with poor water solubility (8-10), poor gastrointestinal permeability (11), or poor oral bioavailability (8,9,(12)(13)(14)(15) to the small intestine. MS-based oral drug delivery systems (ODDSs) can be made from biodegradable polymers (12,(16)(17)(18), nondegradable polymers (19-21), and polysaccharides (22, 23) and can be engineered to carry polypeptides (18,24,25) and other molecules (26, 27). The motivation for using microparticulate-based ODDSs is to improve the oral bioavailability of drugs by enhancing their delivery and transit through the absorptive epithelium of the small intestine. This work may ultimately enable patients to take medications orally instead of relying on daily or weekly injections or other, less convenient routes of administration (28).More than five decades after Sanders and Ashworth discovered that the small intestine can engulf large particles (1), little is known today about the cellular mechanisms or physiologic pathways that facilitate the absorption, transit, and biodistribution of microparticles that are delivered to the small intestine. Nonetheless, the diverse physiology and multitude of cell types in the small intestine likely play a central role in these processes. The absorp...

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The transport of microspheres from the gastro-intestinal tract to inflammatory air pouches in the rat

Cited by 77 publications

References 6 publications

Targeted nanoparticle-based drug delivery and diagnosis

Targeted nanoparticle-based drug delivery and diagnosis

In vitro studies of the interaction of poly(NIPAm/MAA) nanoparticles with proteins and cells

Unique insights into the intestinal absorption, transit, and subsequent biodistribution of polymer-derived microspheres

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